Ab179511

Manufactured by Abcam

Sourced in United States

Ab179511 is a monoclonal antibody that can be used for the detection of a specific target protein. The antibody is produced in mice and has been purified from cell culture supernatant.

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Lab products found in correlation

3 protocols using ab179511

Western Blot Analysis of Neuronal Markers

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Cells were lysed in RIPA buffer and total protein was extracted. Then, the protein concentration was determined by BCA assay. A 10% SDS-PAGE gel (Asegene, Guangzhou, China) was loaded with 20 μg of total protein, and the separated proteins were transferred by electroblotting to PVDF membranes. The membranes were blocked with 5% nonfat dry milk in TBST (50 mM Tris, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) and then incubated with the primary antibody overnight at 4°C. Antibodies included anti-Fut9 (ab176794; 1:500; Abcam, Cambridge, UK), anti-NF200 (18934-1-AP; 1:1000; Proteintech, Chicago, USA), anti-β3-tubulin (ab18207, 1:1000; Abcam), anti-MAP2 (AF4081; 1:1000; Affinity Biosciences, Cincinnati, USA), anti-Hes1 antibody (ab108937; 1:1000; Abcam), anti-Hes5 antibody (ab194111; 1:1000; Abcam), anti-β-tubulin (ab179511; 1:1000; Abcam), and anti-GAPDH (AF7021; 1:1000; Affinity Biosciences). Blots were washed three times with TBST for 5 min each time, and membranes were incubated with HRP-conjugated goat anti-rabbit IgG antibody (ab6721; 1:5000; Abcam) for 1 h at 25°C. Then, the protein bands were visualized with Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich, St Louis, USA).

Chen J., Zeng X., Zhang W., Li G., Zhong H., Xu C., Li X, & Lin T. (2023). Fucosyltransferase 9 promotes neuronal differentiation and functional recovery after spinal cord injury by suppressing the activation of Notch signaling: Fucosyltransferase 9 promotes neuronal differentiation. Acta Biochimica et Biophysica Sinica, 55(10), 1571-1581.

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Differential Gene Expression in CRC

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Our in‐house CRC cohort included 37 pairs of fresh CRC tumor and adjacent normal tissue specimens without radiotherapy or chemotherapy, which were immediately stored in liquid nitrogen after surgery (Table S1). All specimens were collected from Zhengzhou Central Hospital Affiliated to Zhengzhou University between 2019 and 2020 and this study was approved by the Zhengzhou Central Hospital Affiliated to Zhengzhou University. All patients had undergone rigorous screening and underwent informed consent.
qRT‐PCR was employed to detect the RNA levels of CEBPB, ENO1 and β‐Actin. Table S2 lists the involved primer sequences. Table S3 shows the relative expression level of CEBPB and ENO1 mRNA in 37 pairs of CRC tumors and matched to adjacent normal samples. Western blotting was used to detect the protein levels of corresponding genes in 10 pairs of tumors and the matched adjacent normal samples from our center. Primary antibodies used in this study included anti‐β‐Actin (Proteintech, HRP6008), anti‐β‐Tubulin (Abcam, ab179511), anti‐CEBPB (Abcam, ab32358), and anti‐ENO1(CST, 3810T). Table S4 shows the relative expression levels of CEBPB and ENO1 proteins in 10 pairs of CRC tumors and the matched adjacent normal samples (see more in Appendix S1).

Zhang Y., Li L., Chu F., Wu H., Xiao X., Ye J, & Li K. (2024). Itraconazole inhibits tumor growth via CEBPB‐mediated glycolysis in colorectal cancer. Cancer Science, 115(4), 1154-1169.

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Comprehensive Protein Expression Analysis

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Total protein was extracted using RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) and detected utilizing BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). 30 µg of protein per sample was separated by SDS‐PAGE, and then transferred onto PVDF membrane (Gene Molecular Biotech, Inc., Shanghai, China). After obturated with 5% milk for 2 h at room temperature, the membrane was incubated overnight at 4 °C with primary antibodies as follow: COX6C (2 µg mL⁻¹, Abcam, ab110267), DHRS2 (0.1 µg mL⁻¹, Abcam, ab220483), β‐Catenin (1:5000, Abcam, ab32572), Vimentin (1:1000, Abcam, ab92547), E‐cadherin (1:10 000, Abcam, ab40772), Snail (1:1000, Abcam, ab216347), and β‐tubulin (1:1000, Abcam, ab179511). Afterward, the membrane was incubated with HRP‐conjugated goat anti‐rabbit IgG secondary antibodies (1:7500, Abcam, ab205718) or HRP‐conjugated goat anti‐mouse IgG secondary antibodies (1:7500, Abcam, ab205719) for 1 h at room temperature, the expression level was measured with an ECL kit (Roche Diagnostics, Basel, Switzerland) by Western blot imaging system.

Liu Y., Ge J., Chen Y., Liu T., Chen L., Liu C., Ma D., Chen Y., Cai Y., Xu Y., Shao Z, & Yu K. (2023). Combined Single‐Cell and Spatial Transcriptomics Reveal the Metabolic Evolvement of Breast Cancer during Early Dissemination. Advanced Science, 10(6), 2205395.

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