Facs aria iitm
The BD FACS Aria-IITM is a high-performance cell sorter designed for advanced flow cytometry applications. It features a multi-laser optical system and high-speed cell sorting capabilities for precise analysis and sorting of complex cell populations.
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6 protocols using facs aria iitm
Tissue Macrophage Isolation from Infected Mice
Leishmania Infection Immune Response
Isolation of Immune Cells from Murine Leishmaniasis Model
Splenic DC Infection and Parasite Load in Aged Mice
In a separate experiment the splenic DCs were sort selected. Single-cell suspensions were prepared from spleens, and RBCs were lysed using ACK lysing buffer. Dendritic cells were enriched using pan dendritic cell isolation kit (Miltenyi Biotec) and subsequent sort selecting of Leishmania (RFP/m-Cherry) infected splenic DCs and uninfected splenic DCs from different age groups of infected mice were done by high speed FACS cell sorter system (BD FACS Aria-IITM). Infected splenic DCs were sorted by gating live single enriched DCs for Cd11c+Ly6G-Cd11b-RFP/m-Cherry+. Uninfected splenic DCs after enrichment [Cd11c+ Ly6G-Cd11b- RFP/ m-Cherry-] were also sort selected.
Isolation and Purification of Human Immune Cells
Isolation and Culture of DCreg from Monocytes
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