Rabbit anti phospho vegfr2

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit-anti-phospho-VEGFR2 is a primary antibody that recognizes the phosphorylated form of VEGFR2, a receptor tyrosine kinase involved in vascular endothelial growth factor (VEGF) signaling. It is used for the detection and analysis of phosphorylated VEGFR2 in various biochemical and immunological applications.

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VEGFR2 (164 citations) Anti-VEGFR2 (73 citations) Phospho-VEGFR2 (17 citations) Anti-phospho-VEGFR2 (13 citations) Rabbit anti-VEGFR2 (13 citations) Rabbit anti-phospho-VEGFR2 (Y1175), (2 citations)

3 protocols using rabbit anti phospho vegfr2

VEGFR2 and NFAT Signaling in Endothelial Cells

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Antibodies: goat-anti-VEGFR2 (R&D Systems, Minneapolis, MN, USA), goat-anti-VEGFR1 (R&D Systems), rabbit-anti-ERK1/2, mouse-anti-phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴), rabbit-anti-p38, rabbit-anti-phospho-p38 (Thr¹⁸⁰/Tyr¹⁸²), rabbit-anti-phospho-VEGFR2 (Tyr¹¹⁷⁵), rabbit-anti-eNOS, rabbit-anti-phospho-eNOS(Ser¹¹⁷⁷), rabbit-anti-phospho-PLCγ1(Tyr⁷⁸³), rabbit-anti-NFATc2 (Cell Signaling Technology, Danvers, MA, USA), mouse anti-PECAM-1 (CD31), rabbit-anti-PLCγ1 (Santa Cruz Biotechnology, Dallas, TX, USA) and mouse-anti-α-tubulin (Sigma-Aldrich, Poole, UK). Reagents: Endothelial cell growth medium (ECGM) was from PromoCell (Heidelberg, Germany). Scrambled and NFATc2 siRNA duplexes were purchased as siGENOME SMARTpools from Dharmacon (Thermo Scientific, Lafayette, USA). Recombinant human VEGF-A₁₆₅ was from Genentech Inc. (San Francisco, CA, USA) and VEGF-A₁₂₁ was from Promocell.

Fearnley G.W., Bruns A.F., Wheatcroft S.B, & Ponnambalam S. (2015). VEGF-A isoform-specific regulation of calcium ion flux, transcriptional activation and endothelial cell migration. Biology Open, 4(6), 731-742.

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Endothelial Cell Signaling Pathway Analysis

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Commercial antibodies used at 1:1,000 dilution, unless otherwise indicated, includes: mouse anti phospho-eNOS (S1177) (BD Biosciences, 612393; 1:500), mouse anti-eNOS (Abcam, Ab76198), mouse anti-GAPDH (Merk, MAB374; 1:2,000), rabbit anti-Grb2 (Santa Cruz, sc-255), rabbit anti-PI3Kp85 (Millipore, 06-195), and mouse anti–VE-cadherin (Santa Cruz, sc-9989; 1:500). Rabbit anti–phospho-Akt (4858), rabbit anti-Akt (9272), rabbit anti–phospho-FAK (Tyr576/577) (3281s; 1:500), rabbit anti-FAK (3285s), rabbit anti–Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (9101s), mouse anti–p44/42 MAPK (Erk1/2) (4696s), rabbit anti–phospho-PLCγ1 (Tyr783) (2821s), rabbit anti-PLCγ1 (2822s), rabbit anti-PLCγ2 (3872), rabbit anti–phospho-SFK (Tyr416) (2101s; 1:500), rabbit anti-Src (2123s), rabbit anti-phospho-VEGFR2 (Tyr951) (2471s; 1:500), rabbit anti–phospho-VEGFR2 (Tyr1175) (2478s; 1:500), rabbit anti–phospho-VEGFR2 (Tyr1212) (2477s; 1:500), and rabbit anti-VEGFR2 (2479s) were all purchased from Cell signaling technology. Secondary antibodies used were HRP-conjugated anti-rabbit (Cytiva, NA934; 1:10,000) and anti-mouse IgG (Cytiva, NA931; 1:10,000).

Sjöberg E., Melssen M., Richards M., Ding Y., Chanoca C., Chen D., Nwadozi E., Pal S., Love D.T., Ninchoji T., Shibuya M., Simons M., Dimberg A, & Claesson-Welsh L. (2023). Endothelial VEGFR2-PLCγ signaling regulates vascular permeability and antitumor immunity through eNOS/Src. The Journal of Clinical Investigation, 133(20), e161366.

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Endothelial Cell VEGF Signaling Pathway

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Antibodies were goat anti-VEGFR2 (R&D Systems, Minneapolis, MN), rabbit anti-ERK1/2, mouse anti–phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴), rabbit anti-p38, rabbit anti–phospho-p38 (Thr¹⁸⁰/Tyr¹⁸²), rabbit anti–phospho-VEGFR2 (Tyr¹¹⁷⁵), rabbit anti–ATF-2, rabbit anti–phospho-ATF-2 (Thr⁷¹), rabbit anti-NRP1 (Cell Signaling Technology, Danvers, MA), mouse anti–α-tubulin, mouse anti–PECAM-1 (CD31; Santa Cruz Biotechnology, Santa Cruz, CA), and mouse anti–VCAM-1 (DAKO, Glostrup, Denmark).
Reagents were as follows. Endothelial cell growth medium (ECGM) was from PromoCell (Heidelberg, Germany). Scrambled, ATF-2, NRP1, and VCAM1 siRNA duplexes were purchased as siGENOME SMARTpools from Dharmacon (Thermo Scientific, Lafayette, CO) unless otherwise stated in the figure legends. Recombinant human VEGF-A₁₆₅ was from Genentech (San Francisco, CA), and VEGF-A₁₂₁ was from Promocell.

Fearnley G.W., Odell A.F., Latham A.M., Mughal N.A., Bruns A.F., Burgoyne N.J., Homer-Vanniasinkam S., Zachary I.C., Hollstein M.C., Wheatcroft S.B, & Ponnambalam S. (2014). VEGF-A isoforms differentially regulate ATF-2–dependent VCAM-1 gene expression and endothelial–leukocyte interactions. Molecular Biology of the Cell, 25(16), 2509-2521.

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