Log In Sign up
The largest database of trusted experimental protocols

Paav mincmv mcherry vector

Manufactured by Addgene
Visit Supplier

The PAAV-minCMV-mCherry vector is a plasmid designed for the expression of the mCherry fluorescent protein under the control of a minimal CMV promoter. The vector is intended for use in adeno-associated virus (AAV) production and gene expression studies.

Automatically generated - may contain errors

Lab products found in correlation

Pg5luc vector, by Promega (2 mentions)

2 protocols using paav mincmv mcherry vector

1

Cloning TIMP Promoters for Reporter Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
A 1900 bp TIMP-1 promoter sequence spanning from 1026 bp upstream of the start ATG through the 9 bp of exon 2 was synthesized (GenScript, Piscataway, NJ, USA) and subcloned into the pGL4.10 (Promega, Madison, WI, USA) promoter-less firefly luciferase reporter vector. A similar promoter sequence driving the expression of a lacZ reporter was shown to reproduce the spatial and temporal expression patterns of TIMP1 gene in a mouse embryo.15 (link) TATA-containing 1158 bp TIMP-2 and 1154 bp TIMP-3 promoter sequences located immediately upstream of the start codon reported to exhibit promoter activity16 (link),17 (link) were synthesized and subcloned upstream of firefly luciferase reporter vector. The promoter sequence for mouse TIMP-2 was deduced from the human sequence. The 3 TIMP promoter sequences are listed in Table S1. The 5xGAL4 sequence polymerase chain reaction (PCR) amplified from the pG5luc vector (Promega) flanked by the Mlu I and Bam HI restriction enzyme sites was subcloned upstream of the minCMV.mCherry sequence in the pAAV-minCMV-mCherry vector (Addgene #27,970) to create the control GAL4-mCherry reporter.
Duellman T., Doll A., Chen X., Wakamiya R, & Yang J. (2017). dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases. Metalloproteinases in medicine, 4, 63-73.
+ Open protocol
+ Expand
2

Cloning TIMP Promoters for Reporter Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
A 1900 bp TIMP-1 promoter sequence spanning from 1026 bp upstream of the start ATG through the 9 bp of exon 2 was synthesized (GenScript, Piscataway, NJ, USA) and subcloned into the pGL4.10 (Promega, Madison, WI, USA) promoter-less firefly luciferase reporter vector. A similar promoter sequence driving the expression of a lacZ reporter was shown to reproduce the spatial and temporal expression patterns of TIMP1 gene in a mouse embryo.15 (link) TATA-containing 1158 bp TIMP-2 and 1154 bp TIMP-3 promoter sequences located immediately upstream of the start codon reported to exhibit promoter activity16 (link),17 (link) were synthesized and subcloned upstream of firefly luciferase reporter vector. The promoter sequence for mouse TIMP-2 was deduced from the human sequence. The 3 TIMP promoter sequences are listed in Table S1. The 5xGAL4 sequence polymerase chain reaction (PCR) amplified from the pG5luc vector (Promega) flanked by the Mlu I and Bam HI restriction enzyme sites was subcloned upstream of the minCMV.mCherry sequence in the pAAV-minCMV-mCherry vector (Addgene #27,970) to create the control GAL4-mCherry reporter.
Duellman T., Doll A., Chen X., Wakamiya R, & Yang J. (2017). dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases. Metalloproteinases in medicine, 4, 63-73.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!