Flp-InTM T-RExTM293 cells (Thermo Fisher, Cat#R78007) harboring IC2C wt or ∆ICN with C-terminal SNAP-FLAG-strepII tagwhich were generated using the FLP/FRT system (Thermo Fisher). Briefly, pcDNA5-FRT-TO construct and pOG44, which expresses Flipase, were co-transfected using Lipofectamine 2000 (Thermo Fisher) into Flp-InTM T-RExTM293 cells. After recovery from transfection, cells were grown in DMEM containing 10% FBS, 1% Penicillin-Streptomycin, and 100 µg/mL Hygromycin B to select cells in which pcDNA5 construct was inserted into FRT locus. The expression of tagged IC2C was verified by western blotting with antibodies against StrepII tag (Novus Biologicals). These cell lines were grown in culture vessels (Corning, Fisher Scientific) to 80% confluence, and then tagged IC2C expression was induced with 1 µg/ml doxycycline for two days. Cells were harvested in PBS by tapping the culture vessels, and spun down at 1200 rpm for 5 min in Sorvall Legend XTR. Cell pellets were washed with PBS, snap frozen in liquid nitrogen, and stored at −80 °C.
Antibodies against strepii tag
Manufactured by Novus Biologicals
Antibodies against StrepII tag are laboratory reagents used to detect and purify proteins that have been engineered to contain a StrepII affinity tag. The StrepII tag is a short peptide sequence that can be fused to a protein of interest, allowing for its specific recognition and isolation from complex samples. These antibodies provide a reliable and efficient tool for researchers working with StrepII-tagged proteins.
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Lab products found in correlation
2 protocols using antibodies against strepii tag
1
Engineered Flp-In T-REx 293 Cell Lines
2
Flp-In T-REx 293 Cell Line Generation
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Flp-In™ T-REx™293 cells (Thermo Fisher) harboring IC2C wt or ΔICN with C-terminal SNAP-FLAG-strepII tagwhich were generated using the FLP/FRT system (Thermo Fisher). Briefly, pcDNA5-FRT-TOconstruct and pOG44, which expresses Flipase, were co-transfected using Lipofectamine 2000 (Thermo Fisher) into Flp-In™ T-REx™293 cells (Thermo Fisher). After recovery from transfection, cells were grown in DMEM containing 10% FBS, 1% Penicillin-Streptomycin, and 100 μg/mL Hygromycin B to select cells in which pcDNA5 construct was inserted into FRT locus. The expression of tagged IC2C was verified by western blotting with antibodies against StrepII tag (Novus Biologicals). These cell lines were grown in culture vessels (Corning, Fisher Scientific) to 80% confluence, and then tagged IC2C expression was induced with 1 μg/ml doxycycline for two days. Cells were harvested in PBS by tapping the culture vessels, and spun down at 1200 rpm for 5 min in Sorvall Legend XTR. Cell pellets were washed with PBS, snap frozen in liquid nitrogen, and stored at −80°C.
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