Considering HAUSP is a stable protein, for the required efficient reduction of HAUSP, we transfected target cells with two to three-consecutive rounds of siRNA transfection. Cells were seeded around 30-40% confluency, and 24 hours later transfected with the first round. 24 hours later cells were split back to 30-40% confluency. 24 hours later, the second round of siRNA was transfected. Ablation of HAUSP was performed by transfecting indicated adhesive cell lines with Silencer select pre-designed siRNA duplex oligos (Oligo 1: 5’-AAGCGUCCCUUUAGCAUUA-3’; Oligo 2: 5’-GCAUAGUGAUAAACCUGUA-3’; Oligo 3: 5’-UAAGGACCCUGCAAAUUAU-3’; Oligo 4: 5’-GUAAAGAAGUAGACUAUCG-3’) synthesized by Dharmacon. siRNA duplexes for N-Myc (L-003913-01-0005) and control (D-001810-10-0050) were also purchased from Dharmacon. RNAi transfections were performed using Lipofectamine2000 following the manufacturer's protocol.
Silencer select pre designed sirna duplex oligos
Manufactured by Horizon Discovery
Silencer Select pre-designed siRNA duplex oligos are short, double-stranded RNA molecules designed to target and silence specific genes. They are used for gene knockdown studies in cell-based research.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using silencer select pre designed sirna duplex oligos
1
Efficient Depletion of HAUSP Protein
2
Efficient Depletion of HAUSP Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Considering HAUSP is a stable protein, for the required efficient reduction of HAUSP, we transfected target cells with two to three-consecutive rounds of siRNA transfection. Cells were seeded around 30-40% confluency, and 24 hours later transfected with the first round. 24 hours later cells were split back to 30-40% confluency. 24 hours later, the second round of siRNA was transfected. Ablation of HAUSP was performed by transfecting indicated adhesive cell lines with Silencer select pre-designed siRNA duplex oligos (Oligo 1: 5’-AAGCGUCCCUUUAGCAUUA-3’; Oligo 2: 5’-GCAUAGUGAUAAACCUGUA-3’; Oligo 3: 5’-UAAGGACCCUGCAAAUUAU-3’; Oligo 4: 5’-GUAAAGAAGUAGACUAUCG-3’) synthesized by Dharmacon. siRNA duplexes for N-Myc (L-003913-01-0005) and control (D-001810-10-0050) were also purchased from Dharmacon. RNAi transfections were performed using Lipofectamine2000 following the manufacturer's protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!