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Smartpool on targetplus prrx1

Manufactured by Horizon Discovery

The SMARTpool ON-TARGETplus PRRX1 is a pool of siRNA reagents designed to efficiently target the PRRX1 gene. The product is intended for use in RNA interference (RNAi) studies.

Automatically generated - may contain errors

2 protocols using smartpool on targetplus prrx1

1

Silencing PRRX1 in Human Fetal Epicardial Cells

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Cells were treated with SMARTpool ON-TARGETplus PRRX1 or a non-targeting control siRNA according to the manufacturer's protocol at a concentration of 25 nM (Dharmacon). After 48 h, cells were collected for qPCR or western blot. All experiments in human fetal epicardial cells were performed with three or four individual cell isolations.
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2

Knockdown of PRRX1 in Fetal Epicardial Cells

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Human fetal epicardial cells were treated with SMARTpool ON-TARGETplus PRRX1 or a nontargeting control siRNA according to the manufacturers' protocol in a concentration of 25 nM (Dharmacon). After 48 hours, cells were collected for qPCR. All experiments were performed with three or four individual cell isolations.
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