Secondary peroxidase conjugated goat anti mouse antibody

Extraction of total soluble proteins, SDS-PAGE, and Western blot analysis were performed as described [31 (link)]. For detection of PVX, proteins were extracted from systemically infected leaves of N. benthamiana plants infected with PVX or PVX recombinant constructs. The anti-CP monoclonal antibody (MAb) raised against PVX (raised in our lab, Institute of Biotechnology, Zhejiang Univiersity, Hangzhou, China) was used at a 1:8000 dilution. For detection of GFP, proteins were extracted from infiltrated patches of N. benthamiana line 16c or N. benthamiana plants. The anti-GFP MAb (Roche) was used at a 1:8000 dilution. Western blots were visualised with a secondary peroxidase-conjugated goat antimouse antibody (Cell Signaling Technology, Boston, MA, USA) and a chemiluminescence detection system (Tianneng, Shanghai, China).