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Click it edu alexa fluor

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The Click-iT Edu Alexa Fluor is a fluorescent labeling reagent used for the detection and quantification of newly synthesized DNA in cells. It enables the visualization and analysis of cell proliferation through the incorporation of a modified nucleoside into DNA during active DNA synthesis.

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Lab products found in correlation

Prolong gold antifade with dapi, by Thermo Fisher Scientific (2 mentions) Epics xl 4 color cytometer, by Beckman Coulter (2 mentions) Dm5000b, by Leica (1 mentions) Dm 5000b fluorescence microscope, by Leica (1 mentions)

Close spelling variants of this product

Click-iTTM Plus EdU Alexa FluorTM 647 flow cytometry assay kit Click-iTTM EdU Alexa FluorTM 647 Flow Cytometry Assay Kit Click-iTTM EdU Alexa Fluor® 488 Imaging kit Click-iT EdU Alexa Fluor 647 Imaging Kit Click-iT® Plus EdU Alexa Fluor® 555 Imaging Kit Click-iT Plus EdU Cell Proliferation Kit-Alexa Fluor 555 Click-iT EdU Alexa Fluor High Throughput Imaging Assay Kit Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488 Click-iT EdU Alexa Fluor 594 Imaging Kit Click-iT EdU Alexa Fluor 488 flow cytometry assay

5 protocols using click it edu alexa fluor

1

Identification and Pulse-Chase of Proliferating Cells

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Identification and pulse-chase of proliferating cells were performed as described in [1 (link)]. EdU staining was performed using Click-iT™ EdU Alexa Fluor™ [1 (link)] 488 Imaging Kit (C10337, ThermoFisher Scientific) according to the manufacturer's instructions.
Flici H, & Frank U. (2018). Inhibition of SoxB2 or HDACs suppresses Hydractinia head regeneration by affecting blastema formation. Communicative & Integrative Biology, 11(2), 1-5.
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2

Breast Cancer Cell Proliferation Assay

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Breast cancer cells grown on glass coverslips were stained with 10 μmol/L EdU (Click-iT Edu Alexa Fluor; Thermo Fisher Scientific #C10337) according to the manufacturer's instructions. After staining, cells were washed several times with PBS containing 0.5% Triton X-100 and mounted with ProLong Gold antifade with DAPI (Molecular Probes). Fluorescent cells were viewed with a Leica DM5000 B fluorescence microscope. Images were analyzed using ImageJ software.
Zhang J., Pearson A.J., Sabherwal N., Telfer B.A., Ali N., Kan K., Xu Q., Zhang W., Chen F., Li S., Wang J., Gray N.S., Risa-Ebrí B., Finegan K.G., Cross M.J., Giurisato E., Whitmarsh A.J, & Tournier C. (2022). Inhibiting ERK5 Overcomes Breast Cancer Resistance to Anti-HER2 Therapy By Targeting the G1–S Cell-Cycle Transition. Cancer Research Communications, 2(3), 131-145.
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3

Fluorescent Imaging of Cell Proliferation

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Breast cancer cells grown on glass coverslips were stained with 10 μM EdU (Click-iT Edu Alexa Fluor; ThermoFisher #C10337) according to the manufacturer’s instructions. After staining, cells were washed several times with PBS containing 0.5% Triton X-100 and mounted with ProLong Gold antifade with DAPI (Molecular Probes). Fluorescence cells were viewed with a Leica DM5000 B fluorescence microscope. Images were analysed using ImageJ software.
Zhang J., Pearson A.J., Sabherwal N., Telfer B.A., Ali N., Kan K., Xu Q., Zhang W., Chen F., Li S., Wang J., Gray N.S., Risa-Ebrí B., Finegan K.G., Cross M.J., Giurisato E., Whitmarsh A.J, & Tournier C. (2022). Inhibiting ERK5 Overcomes Breast Cancer Resistance to Anti-HER2 Therapy By Targeting the G1–S Cell-Cycle Transition. Cancer Research Communications, 2(3), 131-145.
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4

Quantifying Cell Proliferation via EdU Incorporation

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Cell proliferation was measured by incorporation of fluorescence-conjugated EdU to the newly synthesized DNA, according to the manufacturer’s protocol (Click-iT EdU Alexa Fluor; Invitrogen). Briefly, ovarian cancer cells were plated at a density of 5 × 104 cells in a 6-well dish and transfected with siRNAs. Forty-eight hours after transfection, tumor cells were treated with 10 mM EdU for 2 hr, washed three times with PBS, detached with 0.25% EDTA, fixed with 4% paraformaldehyde for 15 min, immunostained with anti-EdU-FITC (1:200), and analyzed using flow cytometry (EPICS XL 4-Color Cytometer; Beckman Coulter).
Cho M.S., Vasquez H.G., Rupaimoole R., Pradeep S., Wu S., Zand B., Han H.D., Rodriguez-Aguayo C., Bottsford-Miller J., Huang J., Miyake T., Choi H.J., Dalton H.J., Ivan C., Baggerly K., Lopez-Berestein G., Sood A.K, & Afshar-Kharghan V. (2014). Autocrine Effects of Tumor-Derived Complement. Cell reports, 6(6), 1085-1095.
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5

Quantifying Cell Proliferation via EdU Incorporation

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Cell proliferation was measured by incorporation of fluorescence-conjugated EdU to the newly synthesized DNA, according to the manufacturer’s protocol (Click-iT EdU Alexa Fluor; Invitrogen). Briefly, ovarian cancer cells were plated at a density of 5 × 104 cells in a 6-well dish and transfected with siRNAs. Forty-eight hours after transfection, tumor cells were treated with 10 mM EdU for 2 hr, washed three times with PBS, detached with 0.25% EDTA, fixed with 4% paraformaldehyde for 15 min, immunostained with anti-EdU-FITC (1:200), and analyzed using flow cytometry (EPICS XL 4-Color Cytometer; Beckman Coulter).
Cho M.S., Vasquez H.G., Rupaimoole R., Pradeep S., Wu S., Zand B., Han H.D., Rodriguez-Aguayo C., Bottsford-Miller J., Huang J., Miyake T., Choi H.J., Dalton H.J., Ivan C., Baggerly K., Lopez-Berestein G., Sood A.K, & Afshar-Kharghan V. (2014). Autocrine Effects of Tumor-Derived Complement. Cell reports, 6(6), 1085-1095.
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