Sars cov 2 spike protein s1 s2

Normal human primary bronchial epithelial (NHBE) cells from non-obese and obese subjects were purchased from a commercial source (MatTek, MA, USA and ATCC, VA, USA) or obtained from the Biobank of the Quebec Respiratory Health Research Network at the Meakins-Christie Laboratories, Research Institute of the McGill University Health Centre (GLEN site). Table S6 shows the data from the non-obese and obese subjects. NHBE cells were cultured in BEGM media (Lonza, MD, USA) supplemented with 1% antibiotic antimycotic solution (Wisent, QC, CA) in tissue culture 12 well plates coated with Type 1 Rat tail collagen (Sigma-Aldrich, Ontario, Canada). Cells were grown to 90% confluency and starved using BEBM Basal Medium (Lonza, MD, USA) supplemented with 1% antibiotic antimycotic solution (Wisent, QC, CA) over night. The next day cells were stimulated with 1ug/ml of SARS-CoV-2 spike protein (S1+S2) (Sino Biological Inc., Beijing, China) for 3h. Culture media was collected in microtubes, centrifuged 5000g for 5 min. Supernatants were aliquoted into fresh microtubes and frozen at -80°C.

SARS-CoV-2 Spike Protein (S1+S2) (cat# 40589-VO8B1), SARS-CoV-2 Spike RBD Protein (cat# 40592-V08B), SARS-CoV-2 Nucleocapsid Protein (cat# 40588-V08B) were purchased from Sino Biological (China).