Pcdna3.1 myc

Manufactured by Addgene

pcDNA3.1-Myc is a mammalian expression vector that enables the expression of a protein of interest with a Myc epitope tag. The vector contains a cytomegalovirus (CMV) promoter, which drives the expression of the inserted gene, and a Myc tag sequence for detection and purification purposes.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Secondary fluorescent antibodies, by Abcam (1 mentions) H e staining kit, by Abcam (1 mentions) 2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxyglucose 2 nbdg, by Cayman Chemical (1 mentions) Smad2 3 sc133098, by Santa Cruz Biotechnology (1 mentions) 2 deoxy d glucose 2 dg, by Merck Group (1 mentions) Fugene hd, by Promega (1 mentions)

Close spelling variants of this product

PcDNA3.1 (+)/myc-His A vector PcDNA3.1

2 protocols using pcdna3.1 myc

Overexpression of YBX1, Myc, and HIF1α

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For overexpression of YBX1 in T24 cells, or overexpression of Myc and HIF1α in TCCSUP and SW780 cells, pcDNA3.1-Myc (#16011) and pcDNA3.1-HIF1α (#18949) plasmids were acquired from Addgene. In brief, the culture medium was replaced by 600 μl of transfection mixture containing 8 μl of Lipofectamine 2000 and 2 μg overexpressing plasmids or empty vector. Transfection was performed using Lipofectamine 2000 (Invitrogen, Grand Island, NY, USA) according to the manufacturer’s instructions. The overexpression efficiency were assessed after 72 h of transfection

Xu L., Li H., Wu L, & Huang S. (2017). YBX1 promotes tumor growth by elevating glycolysis in human bladder cancer. Oncotarget, 8(39), 65946-65956.

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Multimodal Analysis of Cellular Metabolism

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Antibodies against Glut1 (#SAB4502803), TRAP staining kits and 2-Deoxy-d-glucose (2-DG) were ordered from Sigma. Antibodies against MMP13 (sc-515284), BrdU (sc-32323), ColX (sc-59954), actin (sc-47778), Lamin (sc-377000) and Smad2/3 (sc-133098) were obtained from Santa Cruz Biotechnology. The secondary fluorescent antibodies and H&E staining kit were from Abcam. The fluorescent glucose analog 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) was purchased from Cayman Chemical Company. BrdU labeling, calcein labeling reagents and pSmad2/3 (#PA5-99378) were purchased from Fisher Scientific™. The plasmids pcDNA3.1-Myc and pcDNA3.1-Myc-IFT20 were obtained from Addgene. The transfection reagents (FuGENE® HD) were from Promega Corporation.

Li Y., Yang S., Liu Y., Qin L, & Yang S. (2022). IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism. Redox Biology, 54, 102373.

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