Uv vis detector

Manufactured by Jasco

Sourced in Japan

The UV-vis detector is a laboratory instrument used to measure the absorbance or transmittance of a sample at specific wavelengths of ultraviolet and visible light. It is a core component in various analytical techniques, providing quantitative data about the composition and concentration of the sample.

Automatically generated - may contain errors

Lab products found in correlation

Pu 2080 plus, by Jasco (1 mentions) Lux 5u cellulose 1 column, by Phenomenex (1 mentions) Syringe filter, by Sartorius (1 mentions) Spherisorb ods2, by Waters Corporation (1 mentions) Pu 2080 plus pump, by Jasco (1 mentions) Rf 10axl fluorescence detector, by Shimadzu (1 mentions)

Spelling variants of this product

UV-975 UV/VIS detector (12 citations) UV detector (7 citations) UV-4075 UV/VIS detectors (7 citations) UV-2075 Plus UV/VIS detector (4 citations) UV-2075 UV/Vis detector (3 citations) Intelligent UV–vis detector (3 citations) UV-2075Plus Intelligent UV/Vis Detector (2 citations) UV-1575 UV/Vis Detector (2 citations)

6 protocols using uv vis detector

Validated RP-HPLC Method for Chrysin Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The RP-HPLC method for estimating chrysin content was validated for accuracy, precision, specificity, and solution stability. The method was found to be specific, as observed from the absence of any interfering peaks at the retention time of the analyte. The peak purity of chrysin was 99.99%. The method was found to be linear, in a concentration range of 0.025 to 10 μg/mL, precise (%CV: 1.05–1.36), and accurate (98.9–101.8%). The limit of detection and the limit of quantification were 0.01 and 0.025 μg/mL, respectively. The solution-state stability of chrysin in its mobile phase and phosphate-buffered saline was within an acceptable range (98.5–101.5%) at 20 °C for 1 month and 4 °C for 3 months, respectively.
All test samples were diluted suitably with mobile phase, and chromatographic separation was performed using an isocratic elution. The mobile phase consisted of a mixture of buffer and acetonitrile (45:55) and was delivered at a flow rate of 1 mL/min. The HPLC system consisted of a pump (Jasco PU-2080 Plus, Intelligent HPLC pump, Tokyo, Japan) connected to a detector (Jasco 2075, Intelligent UV–vis detector, Tokyo, Japan). The separation was carried out at 20 °C on a reversed phase C8 column (Agilent, 150 × 4.6 mm, 5 μm particle size). An injection volume of 20 μL was used. Detections were carried out at 270 nm.

Nagaraja S., Basavarajappa G.M., Attimarad M, & Pund S. (2021). Topical Nanoemulgel for the Treatment of Skin Cancer: Proof-of-Technology. Pharmaceutics, 13(6), 902.

+ Open protocol

+ Expand

Chiral HPLC Analysis of Thermal Stability

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chiral HPLC was performed on a JASCO 20XX HPLC system equipped with a semi-preparative Phenomenex Lux-5u-cellulose-1 column (5 μm, 250 × 10 mm). The sample was injected in toluene and eluted with n-hexane/iso-propanol 75:25 at a flow rate of 2.5 mL/min. A JASCO 2070 plus UV-vis detector was used for the detection. For thermal treatment, the concentrated sample was heated in an oil bath to 70 °C and injected to HPLC after various times. Peak areas were determined using PeakFit (seasolve, version 4.12). Fitting was performed after subtracting a background and by using chromatography peak type (exponentially modified Gaussian) with varying peak width and shape. Whereas the standard error for the fitted areas was usually on the order of 1–2% it could be somewhat larger in cases where the background of the chromatogram was not well behaved. Consequently, the standard error for the A/C ratio was typically smaller than 4%.

Wang Y., Nieto-Ortega B, & Bürgi T. (2020). Amplification of enantiomeric excess by dynamic inversion of enantiomers in deracemization of Au38 clusters. Nature Communications, 11, 4562.

+ Open protocol

+ Expand

Optimized Protein Size Exclusion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Size-exclusion chromatography was performed using a Superdex75 10/300 GL column with 25% v/v IPA in PBS. Samples were loaded at 150 μM and monitored at 220 nm using a JASCO UV-vis detector.

Dawson W.M., Lang E.J., Rhys G.G., Shelley K.L., Williams C., Brady R.L., Crump M.P., Mulholland A.J, & Woolfson D.N. (2021). Structural resolution of switchable states of a de novo peptide assembly. Nature Communications, 12, 1530.

+ Open protocol

+ Expand

Histamine Quantification in Tuna Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histamine content was determined according to Duflos et al. [55 (link)] and Chiesa et al. [56 (link)]. Briefly, 4 g of raw or processed tuna samples were weighed and, after adding 250 µL of 1 mM IS and 10 mL of 0.4 M perchloric acid solution, homogenized and centrifuged for 10 min at 2400× g. The supernatant was transferred into a 25 mL bottle through filter paper. The extraction was repeated with 10 mL of 0.4 M perchloric acid solution and centrifuged. The two supernatant aliquots were merged. Derivatization procedure of biogenic amines with dansyl chloride solution (1 mL, 10 mg mL⁻¹ in acetone) was performed at 40 °C for 45 min. Excess of the derivatization reagent was finally neutralized by adding 100 µL ammonia (25%). After 30 min, final extract was adjusted to 5 mL with 0.1 M of ammonium acetate/acetonitrile (1:1) and filtered using a 0.45 µm syringe filter (Sartorius, Goettingen, Germany).
The histamine content was determined using a HPLC Jasco quaternary pump (Ishikawa-cho, Japan) equipped with the autosampler. An adequate elution gradient of ammonium acetate (0.1 M) and acetonitrile was applied as mobile phase with a flow rate of 1 mL min⁻¹. Spherisorb ODS-2, 5 µm, 125 mm × 4 mm (Waters Corporation, Milford, MA, USA) reverse-phase column was used while detection was carried by UV/VIS detector (Jasco, Ishikawa-cho, Japan) operating at 254 nm.

Ghidini S., Chiesa L.M., Panseri S., Varrà M.O., Ianieri A., Pessina D, & Zanardi E. (2021). Histamine Control in Raw and Processed Tuna: A Rapid Tool Based on NIR Spectroscopy. Foods, 10(4), 885.

+ Open protocol

+ Expand

HPLC Analysis of Polyprenols and Plastoquinones

Check if the same lab product or an alternative is used in the 5 most similar protocols

During all steps of the synthesis and chromatographic purification of the compounds, HPLC analysis was used. HPLC was performed using a Nucleosil 100 C18 reverse-phase column (MZ Analysentechnik, Germany, 250 × 4 mm, 5 μm) at a solvent flow rate of 1.5 ml min⁻¹, unless otherwise stated. The HPLC setup included a Jasco PU-2080 Plus pump (Jasco, Tokyo, Japan), a Jasco UV-VIS detector (Jasco, Tokyo, Japan) and Shimadzu RF-10 AXL fluorescence detector (MD, USA) (290/330 nm, excitation/emission). The loop was 100 μl. The absorption detection was at 210 nm for polyprenols or 255 nm for plastoquinones. The solvents used are given in the legends of the chromatograms. During synthesis of the polyprenols, the reaction mixtures were routinely diluted 1000-fold for the HPLC analysis.

Kruk J, & Szymańska R. (2023). Synthesis of natural polyprenols for the production of biological prenylquinones and tocochromanols. RSC Advances, 13(33), 23122-23129.

+ Open protocol

+ Expand

Quantitative Analysis of Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The quantitative estimation of the examined phenolic compounds after the reaction with ABTS radical cation was carried out by HPLC. All measurements were performed using a Gilson with UV-Vis detector, a fluorescence detector (Jasco) and an ODS column (Microsorb MV 100 C18, 15 cm × 4.6 mm i.d.). The sample components were eluted using the following elution program: 0-15 min isocratic elution (5% B) and then gradient of B (5-100%) from 15 to 80 min. Water with acetic acid (5% solution in water) and methanol played the role of solvent A and B, respectively. The samples were injected with a sample injector (Rheonyne 7725) equipped with a 20 µl loop. A wavelength of 254 nm was used for the UV-Vis detector, whereas the fluorescence measurements were performed at λ ex = 278 nm and λ em = 366 nm over 7.0 min in the case of gallic acid, and then (after 7 min) at λ ex = 260 nm and λ em = 420 nm in the case of ferulic and caffeic acids.

Olszowy M., Dawidowicz A.L, & Jóźwik-Dolęba M. (2019). Are mutual interactions between antioxidants the only factors responsible for antagonistic antioxidant effect of their mixtures? Additive and antagonistic antioxidant effects in mixtures of gallic, ferulic and caffeic acids. European food research and technology = Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A, 245(7).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!