Anti cd4 buv496

Manufactured by BioLegend

The Anti–CD4-BUV496 is a fluorochrome-conjugated antibody that specifically binds to the CD4 cell surface antigen. It is designed for use in flow cytometry applications to identify and analyze CD4+ T cells.

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2 protocols using anti cd4 buv496

Characterization of 4-1BBL and Calreticulin Expression in Osteosarcoma

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Viral 41BBL expression in osteosarcoma cell lines was determined by flow cytometry. Cells were stained first with a cell death detection antibody in the Zombie-NIR Fixable Viability Kit (423105, BioLegend) following the manufacturer's protocol and then with a PE-conjugated anti–4-1BBL antibody.
Calreticulin cell surface expression was determined by flow cytometry. Cells were stained first with a cell death detection antibody in the Zombie Green Fixable Viability Kit (423111, BioLegend) and then with a fluorophore-conjugated anti-calreticulin antibody (Abcam).
Fluorescence emission was analyzed using a FACSCanto II system with FACSDiva software (RRID:SCR_001456).
To identify immune cell populations, infiltrating immune cells were surface stained with the following antibody panel: anti–CD45-AF700 (BioLegend), anti–Ly6G-PerCP-Cy5.5 (BioLegend), anti–Ly6C-FITC(BioLegend), anti–F4/80-APC (BioLegend), anti–CD8-BV510 (BioLegend), anti–CD11b-BUV395 (BioLegend), and anti–CD4-BUV496 (BioLegend). PromoFLuor840 maleimide (PromoKine) was used as a viability marker. Samples were acquired with a CytoFlex flow cytometer (Beckman Coulter RRID:SCR_019627) and data analyses were performed using FlowJo v10 (RRID:SCR_008520).

Martinez-Velez N., Laspidea V., Zalacain M., Labiano S., García-Moure M., Puigdelloses M., Marrodan L., Gonzalez-Huarriz M., Herrador G., de la Nava D., Ausejo-Mauleon I., Fueyo J., Gomez-Manzano C., Patiño-García A, & Alonso M.M. (2022). Local Treatment of a Pediatric Osteosarcoma Model with a 4-1BBL Armed Oncolytic Adenovirus Results in an Antitumor Effect and Leads to Immune Memory. Molecular Cancer Therapeutics, 21(3), 471-480.

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Flow Cytometry Analysis of Osteosarcoma Cells

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Viral 41BBL expression in osteosarcoma cell lines was determined by flow cytometry. Cells were stained first with a cell death detection antibody in the Zombie-NIR™ Fixable Viability Kit (423105, Biolegend, San Diego, CA) following the manufacturer’s protocol and then with a PE-conjugated anti-4-1BBL antibody.
Calreticulin cell surface expression was determined by flow cytometry. Cells were stained first with a cell death detection antibody in the Zombie Green Fixable Viability Kit (423111, Biolegend, San Diego, CA) and then with a fluorophore-conjugated anti-calreticulin antibody (Abcam).
Fluorescence emission was analyzed using a FACSCanto™ II system with FACSDiva software (RRID:SCR_001456).
To identify immune cell populations, infiltrating immune cells were surface stained with the following antibody panel: anti-CD45-AF700 (Biolegend, San Diego, CA), anti-Ly6G-PerCP-Cy5.5 (Biolegend, San Diego, CA), anti-Ly6C-FITC (Biolegend, San Diego, CA), anti-F4/80-APC (Biolegend, San Diego, CA), anti-CD8-BV510 (Biolegend, San Diego, CA), anti-CD11b-BUV395 (Biolegend, San Diego, CA), and anti-CD4-BUV496 (Biolegend, San Diego, CA). PromoFLuor840 maleimide (PromoKine) was used as a viability marker. Samples were acquired with a CytoFlex flow cytometer (Beckman Coulter RRID:SCR_019627) and data analyses were performed using FlowJo v10 (RRID:SCR_008520).

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