The-AUY922 compound was kindly provided by Novartis institution for BioMedical Research. Imatinib was purchased from LC laboratories. Imatinib was diluted in PBS and AUY922 was dissolved in 100% DMSO (dimethyl sulfoxide) at the stocking concentration of 10 mM, and was aliquoted into microtubes to be stored at −20°C until use. Commercially available antibodies used were anti-phospho-Jak2Y1007 (Mollipore, Cat# 04-1098), Stat3 (Cell signaling, Cat# 9132), Akt (Cell signaling, Cat# 9272), HSP90 (Cell signaling, Cat# 4874), c-Abl (Cell Signaling, Cat# 2862), phosphotyrosine (4G10) (Millipore, Cat# 05-321), alpha tubulin (B-7) (Santa Cruz Biotechnology, Cat# sc-5286), beta actin (N-21) (Santa Cruz Biotechnology, Cat# sc-130656). Sepharose beads conjugated Jak2 antibody was purchase from Cell Signaling (Cat# 4089). Anti-Abl SH2 domain monoclonal antibody (8E9) was produced by our own lab.
Alpha tubulin b 7
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Alpha tubulin (B-7) is a mouse monoclonal antibody that recognizes the alpha subunit of tubulin, a key structural protein in eukaryotic cells. It is a widely used tool in cell biology research for the detection and study of the tubulin cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alpha tubulin b 7
1
Investigating AUY922 and Imatinib Effects
2
Antibody Characterization for Cellular Analysis
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Hybridoma supernatant against myosin heavy chain (MHC; MF20, deposited by D. A. Fischman) was obtained from the Developmental Studies Hybridoma Bank (University of Iowa, by A. Kawakami). Mouse anti-Brg1 (G-7; sc-17796), anti-Lamin A/C (4A58) and alpha Tubulin (B-7) were obtained from Santa Cruz Biotechnologies (Dallas, TX, USA). Rabbit anti- phospho-Histone H3T6 (AP0093), -Phospho-Histone H3T11 (AP0093), and -Phospho-Histone H3T45 (AP0898) were from Abclonal Technologies (Woburn, MA, USA). Rabbit anti-Pkm1 (D30G6), -Pkm2 (D78A4), Arid1a/Baf250a (D2A8U), -vinculin (E1E9V), and Dpf2 (E7N8J) were from Cell Signaling Technologies (Danvers, MA, USA). Secondary antibodies used for western blot were HRP-conjugated anti-mouse and anti-rabbit (31430 and 31460, respectively) and for immunofluorescence were the goat anti-rabbit IgG Alexa Fluor Plus 488 (A11008) from Thermo Fisher Scientific (Waltham, MA, USA).
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