Temocaprilat

Orlistat, cetilistat, and eslicarbazepine acetate were purchased from Tokyo Chemical Industry (Tokyo, Japan). Temocapril hydrochloride, ampiroxicam, and piroxicam were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). Temocaprilat, irinotecan hydrochloride trihydrate, and SN-38 were purchased from Toronto Research Chemicals (Toronto, Canada). Acebutolol hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO). Eslicarbazepine was purchased from Tocris Bioscience (Bristol, UK). Human liver (n = 50, mixed gender) and intestinal (n = 5, mixed gender) microsomes and monkey liver microsomes (cynomolgus, n = 6, male) were purchased from Corning (Corning, NY). Dog liver (beagle, n = 8, male) and intestinal (beagle, n = 3, male) microsomes were purchased from Xenotech (Lenexa, KS). Recombinant human and monkey CES1, CES2, and AADAC expressed in baculovirus-infected Sf21 cells were previously prepared (Fukami et al., 2010; Watanabe et al., 2010; Honda et al., 2021) . All other reagents were of analytical grade or the highest quality available on the market.

Materials Propranolol hydrochloride, dexamethasone, and bis-p-nitrophenyl phosphate (BNPP) were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). Metoprolol tartrate, atenolol, and acyclovir were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Nadolol and rhodamine 123 were obtained from Sigma-Aldrich Co. (St. Louis, MO, U.S.A.). Tariquidar was obtained from AdooQ BioScience (Irvine, CA, U.S.A.). Temocapril hydrochloride and temocaprilat were obtained from Toronto Research Chemicals, Inc. (North York, ON, Canada). All other chemicals were of the highest available purity.