Dicamba

Manufactured by Duchefa Biochemie

Sourced in Germany

Dicamba is a herbicide product used in laboratory settings. It functions as a synthetic auxin, affecting plant growth and development. The core purpose of this product is for use in controlled research environments, without extrapolation on intended applications.

Automatically generated - may contain errors

Lab products found in correlation

L 1 sucrose, by Merck Group (1 mentions) L 1 agar, by Duchefa Biochemie (1 mentions) Murashige, by Duchefa Biochemie (1 mentions) B5 vitamins, by Duchefa Biochemie (1 mentions)

2 protocols using dicamba

Optimizing Callus Induction in Wheat Embryos

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Excised einkorn and bread wheat mature embryos were used as explants for callus induction. Callus induction media composed of 4.4 g L -1 MS (Murashige and Skoog, 1962) (Duchefa-Haarlem, Netherlands), 30 g L -1 sucrose (Merck Darmstadt, Germany), and 8 g L -1 agar (Duchefa-Haarlem, Netherlands). Agar were supplemented at ve different boron (Sigma-Aldrich-USA) concentrations of 0 to 37.2 mg L -1 (Normally MS media contain 6.2 mg L -1 boron). In addition, four different doses ranging from 0 to 4 mg L -1 of 2,4-D (Sigma-Aldrich, Steinhem, Germany) and Dicamba (Duchefa-Netherlands) were added to callus induction media. The pH of the medium was adjusted to 5.7 to 5.8 using 1 N HCl and 1 N NaOH before autoclaving (NC 40M NUVE-Ankara, Turkey) at 121°C and 1.06 kg cm -2 pressure for 15 min. Subcultures were performed every two weeks.
Detached embryos were placed into callus induction media with scutellum upwards. Each petri dish had 10 embryos in ve replicates. The cultures kept in the growth room at 25±1 °C under dark conditions for four weeks. Callus weight and diameter of calli formed after 4 weeks were measured and recorded.

Ağıl F., Örgeç M., Karakaş F.P., Kumar S.V., & Zencirci N. (2021). In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococum) and bread (Triticumaestivum) wheat under boron stress.

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Optimized Embryo Transformation Protocol

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Immature grains (12−16 d after anthesis) were surface sterilized by immersion for 3 min in 70% (v/v) ethanol, followed by 20 min in 2.4% (w/v) sodium hypochlorite plus 0.1% (v/v) Tween 20 and four rinses in sterile distilled water. The embryos were dissected out aseptically and cultured for 5 d at 24° in the dark on a solid (3 g/L phytagel) medium containing 4.4 g/L Murashige and Skoog salts, 112 g/L B5 vitamins, 30 g/L sucrose, 5 μM copper sulfate, and 5 mg/L dicamba (all chemicals were purchased from Duchefa, Haarlem, The Netherlands). Before bombardment, the callus was transferred onto the same medium supplemented with 0.4 M sorbitol for 4 hr. A 3-mg aliquot of DNA-coated gold particles, prepared as described in the previous paragraph, was used to bombard 20 calli, employing the same device as listed previously, but using a 900 rather than a 1100 psi rupture disk. After bombardment, the material was held for approximately 24 hr at 24° in the dark before being subjected to fluorescence microscopy.

Budhagatapalli N., Rutten T., Gurushidze M., Kumlehn J, & Hensel G. (2015). Targeted Modification of Gene Function Exploiting Homology-Directed Repair of TALEN-Mediated Double-Strand Breaks in Barley. G3: Genes|Genomes|Genetics, 5(9), 1857-1863.

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