Omega plant rna kit
The Omega Plant RNA kit is a laboratory product designed for the extraction and purification of high-quality total RNA from a variety of plant tissues. It utilizes a guanidinium-based lysis solution and silica-based membrane technology to efficiently capture and isolate RNA molecules.
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20 protocols using omega plant rna kit
Quantitative Real-Time PCR Analysis of Gene Expression
RNA-Seq Analysis of Seed Germination
RNA integrity was determined using the Agilent 2100 bioanalyzer (Agilent Technologies). High-quality RNA with an RNA integrity number (RIN) > 8 and of su cient quantity was used to construct the sequencing library. RNA samples were used for poly(A) + selection using oligo (dT) magnetic beads. Then, libraries were sequenced using the Illumina HiSeq TM 4000 platform (Illumina, San Diego, CA, USA) at Gene Denovo Technology Co. Ltd., Guangzhou, China. Raw data of RNA-Seq were collected, and clean data were obtained by removing adapters, unknown nucleotides, and low-quality (Q-value ≤ 10) bases. The Q20, Q30, GC content, and sequence duplication levels of the clean data were simultaneously calculated. High-quality clean data were used for downstream analyses. The clean reads were subsequently de novo assembled using the Trinity software (trinityrnaseq-2.0.6) [61] .
Wheat Homoeolog Expression Patterns
Quantification of WRKY Gene Expression
Transcriptome Analysis of Camellia sinensis
Total RNA was extracted using an Omega Plant RNA kit (Omega Bio-Tek, Code: R6827) according to the manufacturer’s protocol. The quality, integrity and quantity of total RNA were assessed using a NanoDrop 1000 Spectrophotometer (ThermoFisher Scientific, Wilmington, DE, USA) and an Agilent Bioanalyser 2100 with the Agilent RNA 6000 Nano kit (Agilent Technologies, Santa Clara, CA, USA), respectively.
C. arbutifolia Leaf Extraction Protocol
Quantifying Gene Expression in Apple
The species specificity of primers used for analyzing the gene expression of C. mali in apple calli was determined by PCR and gel electrophoresis (
RNA-seq Analysis of Plant Transcriptome
Transcriptome Profiling of GM Maize
Gene expression levels were evaluated in fragments per kilobase million (FPKM) based on the number of fragments mapped to the reference sequence. Differential expression analysis was performed using the DEGseq R package. Genes with an adjusted false discovery rate (FDR) < 0.05 and |log2 fold change|> 1 were scored as differentially expressed.
Wheat Gene Expression Analysis by qPCR
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