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2 protocols using kh2po4

1

Yoghurt Starter Culture and Probiotics

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A commercial yoghurt starter culture (YOFLEX®—consisting of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and three probiotic strains (Lacticaseibacillus rhamnosus LGG, Lacticaseibacillus casei 431, Bifidobacterium animalis subsp. lactis BB-12) were kindly supplied by Chr. Hansen, Bayswater, VIC, Australia. Powdered skim milk (Australian instant skim milk powder, Coles) was obtained from a local supermarket in Melbourne, VIC, Australia. Pancreatin was purchased from Alfa Aesar (Ward Hill, MA, USA). Mangiferin, pyrogallol, gallic acid, caffeic acid, 3-hydroxy phenyl acetic acid, 3-(2-hydroxyphenyl) propionic acid, porcine pepsin, α-amylase from aspergillus oryzae, p-nitrophenyl-α-d-glucopyranoside, bile salts, LC-MS grade formic acid, acetic acid, 4-methyl valeric acid, guar, casein, tryptone, cysteine HCl, peptone, mucin, pectin, potato starch, yeast extract, and MRS agar were acquired from Sigma–Aldrich (Castle Hill, NSW, Australia). Sodium carbonate (anhydrous), HCl, NaHCO3, NaOH, (NH4)2CO3, KCl, NaCl, KH2PO4, MgCl2(H2O)6, CaCl2, K2HPO4, Na3PO4, MgSO4.7H2O Tween 80, orthophosphoric acid, acetonitrile, and methanol of HPLC grade were acquired from Chem-supply Pty Ltd. (Melbourne, VIC, Australia).
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2

Antioxidant and Enzyme Inhibition Assays

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Caffeic acid, quercetin, quercetin-3-galactoside, gallic acid, p-coumaric acid, chlorogenic acid, rutin, luteolin, phloretin, epicatechin, kaempferol-3-glucoside, catechin, quercetin, procyanidin B1, pyrogallol, epicatechin 3′-O-gallate, 3-hydroxybenzoic acid, protocatechuic acid, benzoic acid, cinnamic acid, polydatin, Folin-Ciocalteu phenol reagent (FCR), α-amylase from aspergillus oryzae, porcine pepsin, acarbose, rat intestinal powder, ammonium molybdate, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), p-nitrophenyl-α-d-glucopyranoside, potassium persulfate, bile salts, LC-MS grade formic acid, and HPLC grade acetic acid were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Pancreatin was obtained from Alfa Aesar (Ward Hill, MA, USA). NaCl, Na2CO3, KCl, NaOH, HCl, NaHCO3, (NH4)2CO3, MgCl2(H2O)6, CaCl2, KH2PO4, K2HPO4, Na3PO4, HPLC grade methanol and acetonitrile were purchased from Chem-supply Pty Ltd. (Melbourne, VIC, Australia).
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