Cholangiocarcinoma cell lines (RBE, LIPF155C, LIPF178C, and LICCF) were purchased from the cell back of China Center for Type Culture Collection (CCTCC at Wuhan University). The normal intrahepatic biliary epithelial cell line HIBEpiC was obtained from the ATCC (Manassas, VA, United States).
Hibepic
Manufactured by American Type Culture Collection
Sourced in United States
The HIBEpic is a type of laboratory equipment used for the cultivation and isolation of microorganisms. It provides a controlled environment for the growth and maintenance of various microbial cultures.
Automatically generated - may contain errors
Lab products found in correlation
Hucct1, by American Type Culture Collection (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Hccc9810, by American Type Culture Collection (3 mentions)
Tfk 1, by American Type Culture Collection (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Dharmafect duo transfection reagent, by Thermo Fisher Scientific (1 mentions)
Qbc939, by American Type Culture Collection (1 mentions)
Sh eif5a2, by GenePharma (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Shrna nc, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Thle 3, by American Type Culture Collection (1 mentions)
Hucct1, by National Collection of Authenticated Cell Cultures (1 mentions)
Pcdna3.1 vector, by GenePharma (1 mentions)
Hucct1, by Merck Group (1 mentions)
Huh28, by Merck Group (1 mentions)
Hccc9810, by Merck Group (1 mentions)
Sicrnde 1, by GenePharma (1 mentions)
10 protocols using hibepic
1
Cholangiocarcinoma Cell Lines Acquisition
2
Cell Line Transfection and Cisplatin Assay
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HIBEPIC, HCCC-9810, RBE, TFK-1, and HuCC-T1 cells (ATCC, Manassas, VA, USA) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum. All cell lines were transfected with plasmids using DharmaFECT Duo Transfection Reagent (Thermo Scientific, Waltham, MA, USA) according to the manufacturer's instructions. Vectors expressing short hairpin RNA (shRNA) sequences were provided by Sangon Biotechnology (Shanghai, China). Cisplatin was obtained from Qilu Pharm (Jinan, China) was obtained from Hansoh (Jiangsu, China).
3
Investigating eIF5A's Role in CCA Cells
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CCA cells (HuCCT1, TFK-1, KKU-452, KKU-100, and QBC939) and human intrahepatic biliary epithelial cells (HIBEpiC) were purchased from ATCC. The DMEM medium (Invitrogen, USA) containing 10% fetal bovine serum (Gibco, USA) was used for cells. The culture conditions were 37°C and 5% CO2 in an incubator.
The cells were inoculated into 6-well plates according to the density of 2.5 × 105/well. sh-eIF5A#1 (5′-GCATTACGTAAGAATGGCTTT-3′), sh-eIF5A#2 (5′-GCATTCAAGATGGTTACCTTT-3′), sh-eIF5A#3 (5′-GCCATGTAAGATCGTCGAGAT-3′), shRNA-NC (5′-TTCTCCGAACGTGTCACGT-3′), pcDNA, and pcDNA-eIF5A were obtained from GenePharma (Shanghai, China). As previous studies [23 (link)], after overnight of culture, the serum-free medium was replaced, and then transfection was carried out by lipofectamine 3000 (Invitrogen). After 6 h of culture, medium was replaced; then the cells were cultured for 24 h. Following that, subsequent experiments were performed.
The cells were inoculated into 6-well plates according to the density of 2.5 × 105/well. sh-eIF5A#1 (5′-GCATTACGTAAGAATGGCTTT-3′), sh-eIF5A#2 (5′-GCATTCAAGATGGTTACCTTT-3′), sh-eIF5A#3 (5′-GCCATGTAAGATCGTCGAGAT-3′), shRNA-NC (5′-TTCTCCGAACGTGTCACGT-3′), pcDNA, and pcDNA-eIF5A were obtained from GenePharma (Shanghai, China). As previous studies [23 (link)], after overnight of culture, the serum-free medium was replaced, and then transfection was carried out by lipofectamine 3000 (Invitrogen). After 6 h of culture, medium was replaced; then the cells were cultured for 24 h. Following that, subsequent experiments were performed.
4
Culturing Diverse Cell Lines
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GBC-SD cells were obtained from Cell Bank of the Chinese Academy of Science (Shanghai, China), and G415 cell lines were purchased from RIKEN Cell Engineering Division-Cell Bank (Tokyo, Japan). Human intrahepatic bile duct cell lines (HIBEpiC) were purchased from American Type Culture Collection (ATCC). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) with 10% FBS (Thermo Fischer Scientific), 1% penicillin and streptomycin (Thermo Fisher Scientific) in the condition with 37 °C, 5% CO2.
5
Culturing Liver and Bile Duct Cell Lines
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Immortalized normal liver epithelial cells (THLE3) and human normal bile duct epithelial cell lines (HIBEpiC) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultured as specified by respective manufacturers. Two intrahepatic cholangiocarcinoma cell lines (HuCCT1 and RBE) were acquired from the Cell Bank of the Chinese Academy of Sciences, Shanghai, China. The cell lines were grown at 37 °C under 5% CO2 in a 1640 medium supplemented with 10% fetal bovine serum (FBS, Hyclone, USA). The cells were seeded at a density of 5 × 105 cells in a T25 culture flask and passaged every 4–5 days.
6
Modulation of CRNDE in IHCC Cells
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Normal human intrahepatic biliary epithelial cell (HIBEpic) and four human IHCC cells (HuCCT1, RBE, HCCC9810 and HUH28) were all purchased from the American Type Culture Collection. These cell lines were routinely cultured in DMEM (HIBEpic, HuCCT1, HUH28 and RBE) or RPMI-1640 (HCCC9810), supplemented with 10% fetal bovine serum (FBS) (Sigma, St. Louis, MO, USA), 100 U/ml penicillin sodium, and 100 mg/ml streptomycin sulfate. All cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C.
HuCCT1 cells were transfected with siRNAs targeting CRNDE (siCRNDE-1 and siCRNDE-2) or a scrambled negative control (siNC) (GenePharma, Shanghai, China). HCCC9810 cells were transfected with CRNDE overexpressing vector (pcDNA3.1-CRNDE) or its control vector (pcDNA3.1-Vector) (GenePharma, China). The transfection was conducted with Lipofectamine 2000 (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer's protocol.
HuCCT1 cells were transfected with siRNAs targeting CRNDE (siCRNDE-1 and siCRNDE-2) or a scrambled negative control (siNC) (GenePharma, Shanghai, China). HCCC9810 cells were transfected with CRNDE overexpressing vector (pcDNA3.1-CRNDE) or its control vector (pcDNA3.1-Vector) (GenePharma, China). The transfection was conducted with Lipofectamine 2000 (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer's protocol.
7
Cell Culture of CCA and Biliary Cells
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Human CCA cell lines (RBE and HUCCT1) and normal biliary epithelial cells (HIBEpic) were obtained from the American Type Culture Collection (Manassas, VA) and cultured in In DMEM medium containing 10% fetal bovine serum (DMEM; Invitrogen). The cells were cultured in an incubator at 37 ° C, 5% CO 2 , and 90% relative humidity, and the cells were adherently grown. Fresh medium was replaced every 2-3 day(s) and passaged when the cell fusion reached 80%-90%.
8
Culturing Human Biliary Cell Lines
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Human CCA cell lines (RBE and HUCCT1) and normal biliary epithelial cells (HIBEpic) were obtained from the American Type Culture Collection (Manassas, VA) and cultured in In DMEM medium containing 10% fetal bovine serum (DMEM; Invitrogen). The cells were cultured in an incubator at 37° C, 5% CO2, and 90% relative humidity, and the cells were adherently grown. Fresh medium was replaced every 2–3 day(s) and passaged when the cell fusion reached 80–90%.
9
Intrahepatic Cholangiocarcinoma Cell Culture
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HCCC-9810 and RBE from intrahepatic cholangiocarcinoma and HIBEpiC from normal bile duct epithelium were obtained from American Type Culture Collection. RPMI-1640 containing 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.) was used as complete medium for the above cells, which were cultured at 37°C in humid air under 5% CO2 conditions.
10
Cultivation of Cholangiocarcinoma Cell Lines
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CCA cell lines, RBE and QBC939, were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). The human cholangiocarcinoma cell lines, HuCCT1 and TFK1, were gifted by Kanazawa University in Japan. Normal human bile duct epithelial cell HIBEpic was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Cell lines above were grown in RPMI-1640 medium (GIBCO, Gaithersburg, MD). Cells were supplemented 1% penicillin/streptomycin and 10% fetal bovine serum (GIBCO, Gaithersburg, MD). Cells were incubated at 37 °C with a 5% CO2 atmosphere.
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