Gels 0.5 cm thick were prepared from 1% agarose v/v in 1× TAE buffer (40 mM Tris, 40 mM acetate, 1 mM ethylenedi-aminetetraacetic acid [EDTA]; pH 8.3). A 10 μL amount of the conjugates, 1 μL of 1 mg/mL QD solution, and 1 μL of primary Ab stock solution were mixed with a moderate sample buffer, and the mixtures were loaded into the sample wells on agarose gels in Tris–Tricine sample buffer (Bio-Rad Laboratories Inc, Hercules, CA, USA). The electrophoresis was run at 100 V for 20 minutes in 1× TAE buffer at room temperature and imaged by ultraviolet excitation with the gel-imaging system (Tanon Science and Technology Co, Shanghai, People’s Republic of China). Then the gel was stained with Coomassie Brilliant Blue and imaged with a charge-coupled device (CCD) camera (Kodak, Rochester, NY, USA).
Ccd camera
Manufactured by Kodak
Sourced in United States, Germany
The CCD (Charge-Coupled Device) camera is a type of image sensor used in various laboratory equipment. It functions by converting light into electrical signals that can be processed and captured as digital images. The CCD camera provides a reliable and accurate method of capturing visual data for scientific and analytical applications.
Automatically generated - may contain errors
2 protocols using ccd camera
1
Agarose Gel Electrophoresis of Quantum Dot Conjugates
2
Western Blot Analysis of Antigen Presentation Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proteins were extracted from 1 × 107 cells from the three HNSCC cell lines and protein concentration was determined with the Pierce BCA protein assay kit (Fisher Scientific, Schwerte, Germany). For Western blot analyses, 50 μg protein/lane was separated on 8%-12% SDS-PAGEs, transferred onto nitrocellulose membranes (Schleicher and Schüll, Dassel, Germany) and stained with (3%, w/v) Ponceau S. Immunodetection was performed with specific primary mAbs directed against TAP1, TAP2, LMP2, LMP10, HLA-I HC and β2-m (kindly provided by S. Ferrone, Harvard, Boston, MA, USA) as recently described [8 (link)]. Staining of blots with a GAPDH mAb (Cell Signaling, Frankfurt, Germany) served as loading control. As secondary antibodies the horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies (DAKO, Hamburg, Germany), respectively, were used, and the LumiLight WB substrate (Roche Diagnostics, Mannheim, Germany) was employed for detection and visualization with a CCD camera (Eastman Kodak Co., Berlin, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!