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Ccd camera

Manufactured by Kodak
Sourced in United States, Germany

The CCD (Charge-Coupled Device) camera is a type of image sensor used in various laboratory equipment. It functions by converting light into electrical signals that can be processed and captured as digital images. The CCD camera provides a reliable and accurate method of capturing visual data for scientific and analytical applications.

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2 protocols using ccd camera

1

Agarose Gel Electrophoresis of Quantum Dot Conjugates

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Gels 0.5 cm thick were prepared from 1% agarose v/v in 1× TAE buffer (40 mM Tris, 40 mM acetate, 1 mM ethylenedi-aminetetraacetic acid [EDTA]; pH 8.3). A 10 μL amount of the conjugates, 1 μL of 1 mg/mL QD solution, and 1 μL of primary Ab stock solution were mixed with a moderate sample buffer, and the mixtures were loaded into the sample wells on agarose gels in Tris–Tricine sample buffer (Bio-Rad Laboratories Inc, Hercules, CA, USA). The electrophoresis was run at 100 V for 20 minutes in 1× TAE buffer at room temperature and imaged by ultraviolet excitation with the gel-imaging system (Tanon Science and Technology Co, Shanghai, People’s Republic of China). Then the gel was stained with Coomassie Brilliant Blue and imaged with a charge-coupled device (CCD) camera (Kodak, Rochester, NY, USA).
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2

Western Blot Analysis of Antigen Presentation Proteins

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Proteins were extracted from 1 × 107 cells from the three HNSCC cell lines and protein concentration was determined with the Pierce BCA protein assay kit (Fisher Scientific, Schwerte, Germany). For Western blot analyses, 50 μg protein/lane was separated on 8%-12% SDS-PAGEs, transferred onto nitrocellulose membranes (Schleicher and Schüll, Dassel, Germany) and stained with (3%, w/v) Ponceau S. Immunodetection was performed with specific primary mAbs directed against TAP1, TAP2, LMP2, LMP10, HLA-I HC and β2-m (kindly provided by S. Ferrone, Harvard, Boston, MA, USA) as recently described [8 (link)]. Staining of blots with a GAPDH mAb (Cell Signaling, Frankfurt, Germany) served as loading control. As secondary antibodies the horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies (DAKO, Hamburg, Germany), respectively, were used, and the LumiLight WB substrate (Roche Diagnostics, Mannheim, Germany) was employed for detection and visualization with a CCD camera (Eastman Kodak Co., Berlin, Germany).
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