Crl 1469tm

MDA-MB-231, Panc-1 and HeLa cells were purchased from the American Type Culture Collection (ATCC®) (VA) (HTB-26 TM, CRL-1469TM and CCL-2 TM respectively). Cells were cultured in low-glucose Dulbecco's Modified Medium (DMEM) (Merck, Germany, D6046) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, 10270). MDA-MB-231 cells were cultured at 5% CO₂ concentration and 37 °C and Panc-1 and HeLa were cultured at 10% CO₂ and 37 °C.

Cell lines were obtained from the American Type Culture Collection (ATTCC; Manassas, VA, USA) and comprised U-2 OS human osteosarcoma (ATCC^® HTB-96^TM), HT-29 human colorectal adenocarcinoma (ATCC^® HTB-38^TM), MCF7 human breast adenocarcinoma (ATCC^® HTB-22^TM), PANC-1 human pancreas epithelioid carcinoma (ATCC^® CRL-1469^TM), AsPC-1 human pancreas adenocarcinoma (ATCC^® CRL-1682^TM), IMR-32 human brain neuroblastoma (ATCC^® CCL-127^TM), Hep G2 human hepatocellular carcinoma (ATCC^® HB-8065^TM), A3 acute T cell leukemia (ATCC-CRL-2570), K-562 WT human chronic myelogenous leukemia (ATCC^® CCL-243^TM) and HEK-293 human embryonic kidney (ATCC^® CRL-1573^TM). U-2 OS human osteosarcoma cells stably expressing H2B-mRFP were obtained using the protocol of Fant et al., 2009 [31 (link)]. The cells were maintained as monolayers at 37 °C and 5% CO_2. The culture media, used interchangeably, were RPMI-1640 or DMEM Dulbecco’s modified eagle medium; the media were supplemented with 10 to 15% fetal bovine serum (Life Technologies^TM, Thermo Fisher Scientific, Waltham, MA, USA).