Cytokine concentration was determined using ELISA kits for IL-1β, TNFα, and IL-6 (BioLegend). We purchased a nitric oxide Test Kit from Nanjing Jiancheng Bio to measure nitric oxide production in the supernatant. Briefly, the supernatant from cultured-cells with indicated treatment was collected and centrifuged at 4°C 2,000 g per 15 min, and then was analyzed by following the manufacturer’s instruction.
Nitric oxide test kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Nitric Oxide Test Kit is a laboratory equipment used to measure the levels of nitric oxide (NO) in various samples. It provides a quantitative analysis of NO concentrations through a colorimetric or fluorometric detection method. The kit includes all the necessary reagents and materials to perform the analysis.
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Lab products found in correlation
2 protocols using nitric oxide test kit
1
Cytokine and Nitric Oxide Measurement
2
Nitric Oxide and Oxidative Stress Assay
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Cells were inoculated into the 24-well plate at a density of approximately 1 x 10 5 / mL, and each group was set with 3 parallel wells. After being treated with drugs for 24 h, the supernatant was taken from each BV2 microglial cell group to measure NO levels with a Nitric Oxide Test Kit (Nanjing Jiancheng Bioengineering Institute, China). Oxidative damage was measured by a multifunctional microplate reader (PerkinElmer, USA), and the OD index was calculated according to the manufacturer's instructions.
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