S mutans ua159 bacterial strain

Triethyleneglycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA), 2-methacryloyloxyethyl phosphorylcholine (MPC), methacrylic acid (MA), camphoroquinone (CQ), ethyl-4-(dimethylamino) benzoate (EDMAB), diphenyliodonium hexafluorophosphate (DPIHP), dodecyltrichlorosilane, chlorhexidine digluconate (CHX), N, N-Dimethylformamide (DMF), dichloromethane (DCM) and N-methyl morpholine (NMM) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and were used as received without further purification. γ‒methacryloxypropyl trimethoxysilane (MPS) was used as received from MP Biomedicals (Solon, OH, USA). Piperidine and AlamarBlue Reagent were obtained from Fisher (Thermo Fisher Scientific, Waltham, MA, USA). Rink amide resin, Fmoc-resin, Fmoc-amino acid building blocks, and 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluranium hexafluorophosphate (HBTU) were purchased from AAPPTec LLC (Louisville, KY, USA). BD™ (Becton, Dickinson, and Company; Franklin Lakes, NJ, USA) Bacto™ brain heart infusion, BD BBL dehydrated brain heart infusion agar, and Corning™ (Corning, NY, USA) Clear polystyrene 96-well microplates, Corning™ 3370 were obtained from Fisher. S. mutans UA159 bacterial strain was from American Type Culture Collection (ATCC 700610; Manassas, VA, USA).