SARS-CoV-2 (previous nomenclature: Japan/AI/I-004/2020; present nomenclature: JPN/TY/WK-521) was generously provided from Japan National Institute of Infectious Diseases (Tokyo, Japan). VeroE6/TMPRSS2 cells [13 (link)] were obtained from Japanese Collection of Research Biosources Cell Bank, National Institute of Biomedical Innovation (Osaka, Japan) and maintained in Dulbecco’s modified Eagle’s minimum essential medium (DMEM) (Nissui Pharmaceutical Co. Ltd., Tokyo, Japan) supplemented with G418 disulfate (1 mg/mL), penicillin (100 units/mL), streptomycin (100 μg/mL), 5% fetal bovine serum at 37 °C in a 5% CO2/95% in a humidified atmosphere.
Dulbecco s modified eagle s minimum essential medium dmem
Manufactured by Nissui Pharmaceutical
Sourced in Japan
Dulbecco's modified Eagle's minimum essential medium (DMEM) is a cell culture medium used to support the growth of various cell types. It provides a balanced salt solution with essential nutrients, amino acids, and vitamins required for cell proliferation and maintenance.
Automatically generated - may contain errors
3 protocols using dulbecco s modified eagle s minimum essential medium dmem
1
SARS-CoV-2 Propagation in VeroE6/TMPRSS2 Cells
2
Comparative Analysis of Human Glioma Cell Lines
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Human malignant glioma cell lines A-172 (cat. no. JCRB0228; lot no. 021999), AM-38 (cat. no. IFO50492; lot no. 12082003), T98G (cat. no. IFO50303; lot no. 1007), U-251MG (cat. no. IFO50288; lot no. 12132002) and YH-13 (cat. no. IFO50493; lot no. 1164) were purchased from Health Science Research Resources Bank. The human glioblastoma U-138MG cell line (cat. no. HTB-16; lot no. 1104428) was purchased from the American Type Culture Collection.
Cells were cultured in Dulbecco's modified Eagle's minimum essential medium (DMEM) (Nissui Pharmaceutical, Co., Ltd.) supplemented with 5% fetal calf serum (Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C humidified incubator with an atmosphere containing 5% CO2.
Perampanel was gifted by Eisai Co., Ltd. and TMZ was purchased from Tokyo Chemical Industry Co., Ltd.
Cells were cultured in Dulbecco's modified Eagle's minimum essential medium (DMEM) (Nissui Pharmaceutical, Co., Ltd.) supplemented with 5% fetal calf serum (Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C humidified incubator with an atmosphere containing 5% CO2.
Perampanel was gifted by Eisai Co., Ltd. and TMZ was purchased from Tokyo Chemical Industry Co., Ltd.
3
SARS-CoV-2 Propagation in VeroE6/TMPRSS2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The SARS-CoV-2 (previous nomenclature: Japan/AI/I-004/2020; present nomenclature: JPN/TY/WK-521) were kindly provided from Japan National Institute of Infectious Diseases (Tokyo, Japan) and propagated using VeroE6/TMPRSS2 cells. VeroE6/TMPRSS2 cells [25 (link)] were obtained from Japanese Collection of Research Biosources Cell Bank, National Institute of Biomedical Innovation (Osaka, Japan) and cultured in Dulbecco’s modified Eagle’s minimum essential medium (DMEM) (Nissui Pharmaceutical Co. Ltd., Tokyo, Japan) supplemented with G418 disulfate (1 mg/mL), penicillin (100 units/mL), streptomycin (100 μg/mL), 5% fetal bovine serum at 37 °C in a 5% CO2/95% humidified atmosphere.
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