Waters empower 2 chromatography manager

The size-exclusion chromatography system was a Waters Alliance 2695 Separations Module fitted with a Waters 2996 Photodiode Array Detector (Waters Corporation, Milford, MA, USA). Instrument operation and data acquisition and manipulation were carried out with Waters Empower 2 Chromatography Manager (Waters Corporation). A YMC-Pack Diol-200 column (Product# DL20S05-5008WT, YMC America, Inc., Allentown, PA, USA) with internal dimensions of 500×8.0 mm was used at a flow rate of 0.8 ml/min. The mobile phase consisted of 0.1 M sodium phosphate, 0.15 M sodium chloride (pH 7.0) and peaks were detected at a wavelength of 214 nm.

The size-exclusion chromatography system was a Waters Alliance 2695 Separations Module fitted with a Waters 2996 Photodiode Array Detector (Waters Corporation, Milford, MA, USA). An YMC-Pack Diol-200 column (Product# DL20S05-5008WT, YMC America, Inc., Allentown, PA, USA) with internal dimensions of 500×8.0 mm was used at a flow rate of 0.8 ml/min. The mobile phase consisted of 0.1 M sodium phosphate and 0.15 M sodium chloride (pH 7.0), and peaks were detected at a wavelength of 214 nm. Instrument operation and data acquisition and manipulation were carried out with Waters Empower 2 Chromatography Manager (Waters Corporation) with tabulated results presented as mean +/− standard deviation of 3 separate experiments.