Cells were lysed with RIPA buffer containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (Roche). Protein concentration was quantified with BCA protein assay kit (Pierce) and samples were subjected to SDS-PAGE followed by standard Western blot procedure. Alternatively, sorted cells were directly lysed in SDS reducing sample buffer containing Triton-X100 and βME (beta (2)- Mercaptoethanol) to prepare whole cell lysates. The following antibodies were used for analysis of protein expression: anti-HSF1 (Enzo Life Sciences), p70 S6 Kinase (Cell Signaling), HSF1 phospho-Serine 303 (abcam), HSF1 phospho-Serine 326 (abcam), phospho-S6K Threonine 389 (cell signaling), β-catenin (abcam), β-actin (Sigma-Aldrich), HuR (Abcam), mTOR (Abcam), GAPDH (Santa Cruz). Secondary antibodies used were HRP-goat anti-rat IgG, HRP-goat anti-mouse IgG, HRP-goat anti-rabbit IgG (Santa Cruz).
Hsf1 phospho serine 326
Manufactured by Abcam
HSF1 phospho-Serine 326 is a lab equipment product that detects the phosphorylation of the heat shock factor 1 (HSF1) protein at the serine 326 residue. This modification is important for the regulation of HSF1 activity and the cellular stress response.
Automatically generated - may contain errors
Lab products found in correlation
P70 s6 kinase, by Cell Signaling Technology (2 mentions)
Goat anti mouse igg hrp, by Santa Cruz Biotechnology (2 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (2 mentions)
Anti hsf1, by Enzo Life Sciences (2 mentions)
Hsf1 phospho serine 303, by Abcam (2 mentions)
Phospho s6k threonine 389, by Cell Signaling Technology (2 mentions)
Goat anti rat igg hrp, by Santa Cruz Biotechnology (2 mentions)
β actin, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Roche (2 mentions)
Gapdh, by Santa Cruz Biotechnology (2 mentions)
Phosphatase inhibitor cocktail, by Roche (2 mentions)
β catenin, by Abcam (2 mentions)
2 protocols using hsf1 phospho serine 326
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed with RIPA buffer containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (Roche). Protein concentration was quantified with BCA protein assay kit (Pierce) and samples were subjected to SDS-PAGE followed by standard Western blot procedure. Alternatively, sorted cells were directly lysed in SDS reducing sample buffer containing Triton-X100 and βME (beta (2)- Mercaptoethanol) to prepare whole cell lysates. The following antibodies were used for analysis of protein expression: anti-HSF1 (Enzo Life Sciences), p70 S6 Kinase (Cell Signaling), HSF1 phospho-Serine 303 (abcam), HSF1 phospho-Serine 326 (abcam), phospho-S6K Threonine 389 (cell signaling), β-catenin (abcam), β-actin (Sigma-Aldrich), HuR (Abcam), mTOR (Abcam), GAPDH (Santa Cruz). Secondary antibodies used were HRP-goat anti-rat IgG, HRP-goat anti-mouse IgG, HRP-goat anti-rabbit IgG (Santa Cruz).
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