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Cd11b percp

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CD11b-PerCP is a fluorescently-labeled antibody that binds to the CD11b cell surface antigen. It is commonly used in flow cytometry applications to identify and quantify cells expressing the CD11b marker.

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Lab products found in correlation

F4 80 apc, by Thermo Fisher Scientific (2 mentions) Ly6g pe, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Prism 5, by GraphPad (1 mentions) Cd4 fitc, by Thermo Fisher Scientific (1 mentions) Cellquest pro software, by BD (1 mentions) Ly6g gr1 pe, by Thermo Fisher Scientific (1 mentions) Cellquest pro, by BD (1 mentions) M1 70, by BD (1 mentions) Facs fortessa, by BD (1 mentions) Cd11c apc, by BD (1 mentions) Ly6g fitc, by BD (1 mentions) Nos2 pe, by Thermo Fisher Scientific (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Dnase 1, by Merck Group (1 mentions) Cd45 apc, by Thermo Fisher Scientific (1 mentions) Cd206 apc, by Thermo Fisher Scientific (1 mentions) Cd206 pe, by Thermo Fisher Scientific (1 mentions) F4 80 fitc, by Thermo Fisher Scientific (1 mentions) Cd45 pe, by BioLegend (1 mentions)

Spelling variants of this product

CD11b (360 citations) CD11b-PerCP-Cy5.5 (29 citations) Anti-CD11b PerCP-Cy5.5 (6 citations) Anti-CD11b PerCP cy5.5 (M1/70) (3 citations) Anti-mouse-CD11b/PerCP-Cy5.5 (3 citations) PerCP‐Cy5.5‐anti‐CD11b (3 citations) PERCP-Cy5.5-conjugated anti-CD11b (2 citations) CD11b (M1/70)-PerCP-Cy5.5 (2 citations) CD11b-PerCP-Cy5.5 (M1/70; (2 citations) CD11b PerCP-Cyanine5.5 (2 citations)

4 protocols using cd11b percp

1

Immune Cell Profiling by Flow Cytometry

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Nasal lavage samples (100 µl per mouse) were stained with the following fluorescent antibodies: CD4-FITC, Ly6G-PE, CD11b-perCP and F4/80-APC (eBioscience) after blocking with FC Block at 4°C. Cells were then fixed with 1% paraformaldehyde and assayed using a BD FACSCalibur the following day. Data were gathered using CellQuest Pro software (BD), analyzed using FlowJo software (TreeStar), and graphed with Prism 5 (GraphPad).
Richard A.L., Siegel S.J., Erikson J, & Weiser J.N. (2014). TLR2 Signaling Decreases Transmission of Streptococcus pneumoniae by Limiting Bacterial Shedding in an Infant Mouse Influenza A Co-infection Model. PLoS Pathogens, 10(8), e1004339.
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2

Immune Cell Profiling in Tumor and Lung

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To study variation in immune cellular populations in the tumor and lung microenvironments and systemic immune changes related to performance of a CNB, a single cohort of 60 mice was used. Twenty mice from the biopsy (n = 10) and non-biopsy (n = 10) groups were sacrificed on each assigned post-biopsy time point (days 3, 7, and 10). Sections of tissue from tumor, lung, spleen, and lymph node were harvested and immediately processed and labeled with CD4 − fluorescein isothiocyanate, CD3 − Phycoerythrin (PE), CD8 − PerCP, Dx5 (Pan-Natural Killer [NK])− APC, CD45 − fluorescein isothiocyanate, Ly6G (Gr-1)–PE, CD11b-PerCP, and F4/80-APC antibodies (eBioscience Inc., San Diego, CA) allowing quantification of CD4 + T cells, CD8 + T cells, NK cells, macrophages, and myeloid-derived suppressor cells (MDSCs). Using a FACScalibur flow cytometer (BD Biosciences, CA), data were collected and analyzed using CellQuest Pro (BD Biosciences), FCS express ver. 4 (DeNovo Software, CA), and Flowing Software ver. 2.5 (University of Turku, Turku, Finland).
Mathenge E.G., Dean C.A., Clements D., Vaghar-Kashani A., Photopoulos S., Coyle K.M., Giacomantonio M., Malueth B., Nunokawa A., Jordan J., Lewis J.D., Gujar S.A., Marcato P., Lee P.W, & Giacomantonio C.A. (2014). Core Needle Biopsy of Breast Cancer Tumors Increases Distant Metastases in a Mouse Model. Neoplasia (New York, N.Y.), 16(11), 950-960.
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3

Neutrophil Immunophenotyping by Flow Cytometry

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Single-cell suspensions were stained for surface markers in PBS containing 0.1% sodium azide and 1% BSA for 30 min at 4 °C and fixed with 2% paraformaldehyde. Data was acquired on a BD FACS Fortessa machine (BD Biosystems, UK). Forward scatter and side scatter gates were used to exclude debris and dead cells were excluded using a fixable near IR dead cell stain kit for 633 or 635 nm excitation. Neutrophils were defined as Ly-6Ghigh (Ly6G-FITC; BD Biosciences; clone 1A8), CD11bhigh (CD11b-PerCP; eBioscience; clone M1/70), CD11clow (CD11c-APC; BD Biosciences; clone HL3), F4/80low (F4/80-PE/Dazzle; BD Biosciences; clone BM8).
Low C.M., Akthar S., Patel D.F., Löser S., Wong C.T., Jackson P.L., Blalock J.E., Hare S.A., Lloyd C.M, & Snelgrove R.J. (2017). The development of novel LTA4H modulators to selectively target LTB4 generation. Scientific Reports, 7, 44449.
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4

Tumor and Immune Cell Isolation

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Scissors and tweezers were used to carefully peel off the tumor tissues. After weighing, tumor tissues were harvested and digested with RPMI 1640 medium containing 5% FBS, 2 mg/mL collagenase IV (Sigma, USA) and 5 U/mL DNase I (Sigma, USA) for 20 minutes by a 37 °C shaker. Then the cell suspension was neutralized with RPMI 1640 medium containing 5% FBS and filtered through 70-m nylon mesh into a centrifuge tube to obtain single cell suspension. Bone marrow cells were obtained as described above. Spleens were milled and filtered with PBS into centrifuge tubes through a 200-mesh sieve. Then, the cells were lysed with red blood cell lysate, filtered through gauze, and resuspended in PBS. For immunocyte detection, the following anti-mouse antibodies were used: CD45-PE (103106, BioLegend), CD45-APC (17-0451-83, eBioscience), CD11b-APC (101212, BioLegend), CD11b-PerCP (45-0112-82, eBioscience), Gr-1-FITC (108406, BioLegend), F4/80-FITC (11-4801-82, eBioscience), NOS2-PE (12-5920-82, eBioscience), CD206-PE (12-2061-82, eBioscience), and CD206-APC (17-2061-82, eBioscience). A FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ) was applied to detect the stained cells and the FlowJo software (TreeStar, Ashland, OR) was utilized to analyze the data.
Liu X., Xu Y., Li Y., Pan Y., Zhao S, & Hou Y. (2021). Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype. International Journal of Medical Sciences, 18(14), 3125-3139.
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