Synapsin 1 antibody

Brain cortical tissue of mice on omega-3 adequate and deficient diet groups was excised at 24 h and 7 days after TBI in two separate experiments. The α-spectrin cleavage was assessed at 24 h while synapsin 1 expression was assessed at 7 days after TBI. The tissue was homogenized manually in 2X lysis buffer (Cell Signaling Technology, Danvers, MA, USA) using a Potter-Elvehjem homogenizer, briefly sonicated and centrifuged to obtain the supernatant. Protein content was estimated and cortical samples were run in gradient polyacrylamide gels (Novex, Invitrogen, Carlsbad, CA, USA) and subsequently transferred onto a PVDF membrane (Millipore, Billerica, MA, USA). After blocking with 5% milk, mouse α-spectrin antibody (Santa Cruz Biotechnology, 1∶800) or synapsin-1 antibody (Sigma, 1∶1000) was used to probe for the respective protein, which were detected using Pierce supersignal west pico chemiluminescent detection substrate (Thermo Fisher Scientific, Rockford, IL, USA).