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7 protocols using graphite powder

1

Synthesis of Graphene Oxide Nanocomposite

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Levofloxacin hydrochloride 98%, cellulose nanocrystals and graphite powder 99.95% (for the preparation of GO) were purchased from Shanghai Macklin Biochemical Co., Ltd. Deionized water was used for all experiments. Otherwise specified chemicals were of reagent grade and used without further purification. Deionized water was used throughout the experiments.
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2

Synthesis of Graphene-Based Electrode Materials

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Graphite powder was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. Sulfuric acid (H2SO4, 98%), potassium permanganate (KMnO4), and hydrogen peroxide (H2O2, 30 wt%) were sourced from Xilong Scientific Co., Ltd. Hydrogen acid (HCl) and acetone (C3H6O) were provided by Tianjin Fuyu Fine Chemical Co., Ltd. Ethanol (C2H5OH, 99.5%) and potassium hydroxide (KOH) were purchased from Anhui Senrise Technology Co., Ltd. Potassium nitrate (KNO3) was purchased from Beijing Beihua Fine Chemical Co., Ltd. Urea (CH4N2O), cobalt nitrate hexahydrate (Co(NO3)2·6H2O) and samarium nitrate hexahydrate (Sm(NO3)3·6H2O) were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. Nickel foam (NF) was made available by Kunshan Tonghui Electronic Technology Co., Ltd. All chemicals used were of analytical purity, and deionised water was used in all experiments.
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3

Graphite Electrode Synthesis Protocols

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Graphite powder (average particle diameter 150 μm, 99.95%, metals basis) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Copper mesh (500 mesh) was purchased from Huawei hardware store (Beijing, China). Ag/AgCl electrode and platinum mesh electrode were purchased from Ida Co., Ltd. (Tianjin, China). Tetraoctylammonium bromide (TOAB, purity > 98%) was purchased from J&K Scientific Co., Ltd. (Beijing, China). Dimethylsulfoxide (DMSO, AR grade, 99.9%, dehydrated over a 4 Å molecular sieve prior to use) and anhydrous methanol (AR grade, 99.8%) were purchased from Modern Oriental Technology Development Co., Ltd. (Beijing, China).
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4

Graphite-Cyclodextrin Composite Synthesis

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Graphite powder and β-cyclodextrin (β-CD) were purchased by Shanghai Macklin Biochemical Technology Co. Ltd. Ammonia solution were purchased from Tianjin Hengxing Chemical Reagent Factory. 4-Dimethylaminopyridine (DMAP), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and dopamine (DA) were produced by Aladdin Industrial Corporation of Shanghai. Except that the purity of ammonia water is 25%, other available reagents were of analytical grade without further purification. All solutions were prepared via ultra-pure water purified with the Millipore system from USA.
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5

Graphite-Cerium Nanocomposite Synthesis

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Graphite powder, cerium nitrate, KH570, N,N-dimethylformamide (DMF), glacial acetic acid, sodium hydroxide, anhydrous ethanol, epoxy (E-51), and polyamide curing agent (650) were purchased from Shanghai Macklin Biochemical Co., Ltd., Shanghai, China. Cetyl trimethyl ammonium chloride (CTAC) and TEOS were purchased from Aladdin Biochemical Polytron Technologies Inc. Dimethyl benzene and concentrated sulfuric acid were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Potassium permanganate and hydrogen peroxide were purchased from Xilong Scientific Co., Ltd., Guangzhou, China.
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6

Green Tea-Derived Carbon Electrode Fabrication

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Carbon cloth (CC, W0S1009) was bought from CeTech Co., Ltd (Shanghai, China). Carbon felt with thickness 3 mm was obtained from Hot-Material Co., Ltd, China. Green tea was purchased from Lipton Green Tea Co., Ltd, China. Graphite powder were purchased from Shanghai Macklin Biochemical Co., Ltd. Anaerobic sludge from Shihezi Second Wastewater Treatment Plant (Shihezi, China) was used as the inoculum strain.
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7

Magnetic Nanoparticles for Targeted Cancer Therapy

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Graphite powder, 1,2-ethanediamine (EDA), dimethylformamide (DMF), and hyaluronic acid (HA) were purchased from Macklin (Shanghai, China). Iron chloride tetrahydrate (FeCl2·4H2O) and ferric chloride hexahydrate (FeCl3·6H2O) were bought from the Guangfu Fine Chemical Industry Research Institute (Tianjin, China). β-CD, P-toluenesulfonyl chloride (p-TsCl), 3-aminopropyltriethoxysilane (APTES), N-hydroxysuccinimide (NHS), and 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC) were supplied by Aladdin (Shanghai, China). Doxorubicin hydrochloride (DOX) was purchased from Civi Chemical Technology Co. Ltd. (Shanghai, China). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The human hepatoma cell line BEL-7402 was purchased from the cell bank of the Shanghai Institute for Biological Sciences (Shanghai, China). All the other chemicals were obtained in analytical grades from local suppliers and used without further purification. Deionized water (DI water) was prepared by a Milli-Q Plus system (Millipore, Burlington, MA, USA) and used throughout this experiment.
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