Flag m2 3165

Manufactured by Merck Group

The FLAG (M2-3165) is a laboratory equipment product manufactured by Merck Group. It is designed for specific applications in research and analysis. The core function of this product is to provide a reliable and consistent tool for researchers and scientists. No further details or interpretation on its intended use can be provided in an unbiased and factual manner.

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Lab products found in correlation

Dld 1 cells, by Merck Group (2 mentions) Cenp a and anti ssdna, by Merck Group (2 mentions) Rat anti brdu bu1 75 and fancd2, by Novus Biologicals (2 mentions) Hela cell, by American Type Culture Collection (2 mentions) Bromodeoxyuridine (brdu), by Merck Group (2 mentions) Bromodeoxyuridine (brdu), by BD (2 mentions) β actin antibody, by Santa Cruz Biotechnology (2 mentions) Rpmi 1640, by Corning (1 mentions) Cd9 sc13118, by Santa Cruz Biotechnology (1 mentions) Matrigel 356234, by BD (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) C57bl 6n, by Charles River Laboratories (1 mentions) E cadherin 20874 1 ap, by Proteintech (1 mentions)

Spelling variants of this product

FLAG M2 (383 citations) Mouse monoclonal anti-FLAG (M2) (F-3165, (3 citations)

3 protocols using flag m2 3165

Cell Line Authentication and Characterization

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SW480, SW620, HCT116, DLD1, LOVO, HT29, HEK293, and B16 cell lines were obtained from the ATCC and recently been authenticated by STR profiling. The SW480 cell line was cultured in RPMI 1640 (Corning) supplemented with 10% FBS (PAN, P30-3302), and the SW620, HCT116, DLD1, LOVO, HT29, HEK293 as well as B16 cell lines were cultured in DMEM (Corning) supplemented with 10% FBS. These cell lines were cultured in a 37 °C incubator with 5% (v/v) CO₂.
Mouse monoclonal anti-SEC23B antibody was generated against the synthetic peptide LTKPAMPMQQARPAQPQEHP, and was validated in our laboratory (Supplementary Fig. 1a). The commercial antibodies used in this study included GFP (RM1008), GAPDH (RM2002), and α-Tubulin (RM2007) antibodies from Sungene Biotech; EPCAM (66316-1-AP), E-cadherin (20874-1-AP) and PDI (11245-1-AP) from Proteintech; FLAG (M2-3165) from Sigma-Aldrich; GM130 (A5344) from ABclonal; CD9 (sc13118) from Santa Cruz.
The reagents used in this paper included Fibronectin (354008) from Biocoat, Matrigel (356234) from BD, puromycin (sc-205821A) from Santa Cruz, MG132 (Carbobenzoxy-L-leucyl- L-leucyl-L-leucinal) (MB5137) from EPSILON, and chlorhexidine (CHX) (C7698) from SBJBIO, anti-fade mounting reagent (C1210) from Polygen.
C57BL/6N was purchased from Charles River Laboratories.

Yang C., Chen N., Li X., Lu D., Hou Z., Li Y., Jin Y., Gu J, & Yin Y. (2020). Mutations in the coat complex II component SEC23B promote colorectal cancer metastasis. Cell Death & Disease, 11(3), 157.

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PTEN Regulation of DNA Damage Response

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Pten^+/+ and Pten^−/− mouse embryo fibroblasts (MEFs, provided by P.P. Pandolfi, Harvard Medical School), PTEN^+/+ and PTEN^−/− HCT116 cells (a kind gift from T. Waldman, Georgetown University), PTEN^+/+ and PTEN^−/− DLD-1 cells (from Sigma-Aldrich) and HeLa cells (from ATCC) were cultured in MEM supplemented with 10% FBS. The pSUPER RNAi system (Oligoengine Inc.) was used to construct PTEN-specific shRNA expression plasmids and scrambled control shRNA. PTEN, Rad51 and Chk1 expression plasmids were constructed in a pCMV expression vector. BrdU, IdU (5-Iodo-2′-deoxyuridine), CldU (5-Chloro-2′-deoxyuridine), APH (aphidicolin) and HU (hydroxyurea) were from Sigma-Aldrich. The following antibodies were used: PTEN (A2B1), Rad51 (H92), PCNA (FL-261), Chk1 (G4), PARP1/2 (H250), β-Actin antibodies from Santa Cruz; BrdU from BD Pharmingen; CENP-A and anti-ssDNA from EMD Millipore; Rat anti-BrdU BU1/75 and FANCD2 from Novus; FLAG (M2-3165) from Sigma-Aldrich.

He J., Kang X., Yin Y., Chao K.S, & Shen W.H. (2015). PTEN Regulates DNA Replication Progression and Stalled Fork Recovery. Nature communications, 6, 7620.

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PTEN Regulation of DNA Damage Response

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He J., Kang X., Yin Y., Chao K.S, & Shen W.H. (2015). PTEN Regulates DNA Replication Progression and Stalled Fork Recovery. Nature communications, 6, 7620.

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