All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) and cell culture media was purchased from Invitrogen (Carlsbad, CA) unless otherwise indicated. Arginase Assay Kit was purchased from BioAssay Systems (Hayward, CA), and hybridoma cell lines of F4/80 and Mac-2 from American Tissue Culture Collection (ATCC, Manassas, VA). Anti-IBA-1 (ionized calcium binding adapter molecule 1) antibody was purchased from Wako (Osaka, Japan) and anti-Arginase 1 antibody from BD bioscience. Antibodies of anti-Stat3, phospho-Stat3 (Tyr705), GAPDH, NF-κB p65, P-PI3K p85/p55, P-Akt and Stat6 were purchased from Cell Signaling Technology (Danvers, MA). Antibodies of anti-phospho-Stat6 (Tyr641) and PPARγ were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-YM 1 antibody was purchased from STEMCELL Technologies (Vancouver, BC, Canada). All secondary antibodies were from Invitrogen.
Anti arginase 1 antibody
Manufactured by BD
Sourced in Japan
The Anti-Arginase 1 antibody is a laboratory tool used to detect the Arginase 1 protein, which is involved in the urea cycle. This antibody can be used in various scientific research applications that require the identification and quantification of Arginase 1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Stat6, by Cell Signaling Technology (1 mentions)
Cell culture media, by Thermo Fisher Scientific (1 mentions)
Arginase assay kit, by BioAssay Systems (1 mentions)
Anti ym1 antibody, by STEMCELL (1 mentions)
Ppi3k p85 p55, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Anti iba1, by Fujifilm (1 mentions)
Alexa 488, by Abcam (1 mentions)
2 protocols using anti arginase 1 antibody
1
Immunofluorescence Analysis of Macrophage Markers
2
Immunostaining of Arginase-1 in BALF Cells
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Immunostaining of arginase-1 was performed on BALF cellular infiltrates mounted on cytospin slides. Briefly, BALF cells were first fixed in 4% paraformaldehyde solution, followed by permeabilization with 0.1% Triton X-100 PBS solution and subsequent blocking with 5% BSA solution. Cells were then incubated with anti-arginase-1 antibody (BD Biosciences, cat# 610708) for 1 hour before the application of a secondary antibody (Donkey anti-mouse Alexa 488 (Abcam, Cat# Ab150105) for 30 minutes. Slides were mounted in Prolong-gold/ DAPI before imaging.
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