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Ccr7 fitc

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CCR7-FITC is a fluorescently-labeled antibody that binds to the CCR7 receptor, which is expressed on the surface of certain immune cells. This product can be used for the identification and analysis of CCR7-expressing cells in flow cytometry applications.

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Lab products found in correlation

Cd45ro apc, by Miltenyi Biotec (3 mentions) Cd3 apc vio770, by Miltenyi Biotec (2 mentions) Cd62l vioblue, by Miltenyi Biotec (2 mentions) Cd45ra pe, by Miltenyi Biotec (2 mentions) Cd4 vioblue, by Miltenyi Biotec (2 mentions) Cd56 pe vio770, by Miltenyi Biotec (2 mentions) Cd19 fitc, by Miltenyi Biotec (2 mentions) Cd16 pe, by Miltenyi Biotec (2 mentions) Cd8 viogreen, by Miltenyi Biotec (2 mentions) Cd105 pe, by Miltenyi Biotec (1 mentions) Cd80 apc, by Miltenyi Biotec (1 mentions) Cd107a apc, by Miltenyi Biotec (1 mentions) Ifnγ pe, by Miltenyi Biotec (1 mentions) Epcam vioblue, by Miltenyi Biotec (1 mentions) Hla dr percp, by Miltenyi Biotec (1 mentions) E cadherin apc, by Thermo Fisher Scientific (1 mentions) N cadherin pe, by Abcam (1 mentions) Dnam 1 pe, by BioLegend (1 mentions) Cd133 apc, by Miltenyi Biotec (1 mentions) Cd56 fitc, by Miltenyi Biotec (1 mentions)

4 protocols using ccr7 fitc

1

Multi-Marker Cytometry Profiling

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For cytofluorimetric analysis, cells were stained with surface antibodies in PBS 5% FCS for 20 min at 4 °C. The following antibodies were used: CD133-APC, EpCAM -VioBlue, CD105-PE, CD90-VioBlue, IFNγ-PE, CD107a-APC, CCR7-FITC, CD163-PerCP-Vio700, CD206-VioBlue, HLA-DR-PerCP, CD80-APC (MIltenyi Biotec); CD56-PC7, NKp30-PE (Beckman Coulter); CD29-PE (ImmunoTools); CD146-PC7, CD73-FITC, NKp46-V450 (BD Biosciences); E-cadherin-APC (Thermo Fisher Scientific); N-cadherin-PE (Abcam); DNAM1-PE (Biolegend).
Fiore P.F., Vacca P., Tumino N., Besi F., Pelosi A., Munari E., Marconi M., Caruana I., Pistoia V., Moretta L, & Azzarone B. (2021). Wilms’ Tumor Primary Cells Display Potent Immunoregulatory Properties on NK Cells and Macrophages. Cancers, 13(2), 224.
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2

Phenotyping of T and NK cell subsets

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T cells and NK cells were obtained as described above. Cells were incubated with fluorochrome-labeled antibodies at 4°C. Specific antibodies for the T cell subsets include CD3 V450, CCR7 FITC, CD45RO APC and CD45RA Viogreen (Miltenyi Biotech, Auburn, CA). Specific antibodies for the NK cell subsets include, CD56 FITC, CD11b V450, CD3 Viogreen, and CD27 APC (Miltenyi Biotech, Auburn, CA). Flow cytometry was performed as previously described (19 (link)).
del Campo S.E., Levine K.M., Mundy-Bosse B.L., Grignol V.P., Fairchild E.T., Campbell A.R., Trikha P., Mace T.A., Paul B.K., Jaime-Ramirez A.C., Markowitz J., Kondadasula S.V., Guenterberg K.D., McClory S., Karpa V.I., Pan X., Olencki T.E., Monk J.P., Mortazavi A., Tridandapani S., Lesinski G.B., Byrd J.C., Caligiuri M.A., Shah M.H, & Carson WE I.I.I. (2015). The Raf Kinase Inhibitor Sorafenib Inhibits JAK-STAT Signal Transduction in Human Immune Cells. Journal of immunology (Baltimore, Md. : 1950), 195(5), 1995-2005.
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3

Immunophenotyping of PBMC and T-cells

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Staining of cell surface markers on PBMC and T-cells was performed with CD3-APC/Vio770, CD4-Vioblue, CD8-Viogreen, CD19-FITC, CD56-PE/Vio770, CD16-PE, CD62L-Vioblue, CD45RA-PE, CD45RO-APC, and CCR7-FITC (Miltenyi, San Diego, CA; BD, Franklin Lakes, NJ). All samples were acquired on a MACSQuant Cytometry (Miltenyi) and the data analyzed with Flow Jo (Treestar, Ashland, OR).
McLaughlin L.P., Lang H., Williams E., Wright K.E., Powell A., Cruz C.R., Colberg-Poley A.M., Barese C., Hanley P.J., Bollard C.M, & Keller M.D. (2016). Human Parainfluenza Virus-3 can be Targeted by Rapidly ex vivo Expanded T-Lymphocytes. Cytotherapy, 18(12), 1515-1524.
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4

Comprehensive CBMC and T Cell Immunophenotyping

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Staining of cell surface markers on CBMCs and T cells was performed with CD3-APC/Vio770, CD4-Vioblue, CD8-Viogreen, CD19-FITC, CD56-PE/Vio770, CD16-PE, CD62L-Vioblue, CD45RA-PE, CD45RO-APC, and CCR7-FITC (Miltenyi Biotec and BD Biosciences). 50,000 events per sample were acquired on a MACSQuant cytometer (Miltenyi), and the data were analyzed with Flow Jo (Tree Star).
Dave H., Luo M., Blaney J.W., Patel S., Barese C., Cruz C.R., Shpall E.J., Bollard C.M, & Hanley P.J. (2017). Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood. Molecular Therapy. Methods & Clinical Development, 5, 13-21.
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