Electrophysiological signals were acquired with 32-site polytrode acute probes (A1×32-Poly3–5mm-25s-177, Neuronexus) through an A32-OM32 adaptor (Neuronexus) connected to a Cereplex digital headstage (Blackrock Microsystems). A fiber-attached polytrode probe (A1×32-Poly3–5mm-25s-177-OA32LP, Neuronexus) was used for recordings from optogenetically identified GABAergic cells. Unflltered signals were digitized at 30 kHz at the headstage and recorded by a Cerebus multichannel data acquisition system (BlackRock Microsystems). Experimental events and respiration signals were acquired at 2 kHz by analog inputs of the Cerebus system. Respiration was monitored with a microbridge mass airflow sensor (Honeywell AWM3300V) positioned directly opposite the animal’s nose. Negative airflow corresponds to inhalation and negative changes in the voltage of the sensor output.
A1 32 poly3 5mm 25s 177
Manufactured by NeuroNexus
The A1 × 32-Poly3-5mm-25s-177 is a multi-channel probe for neural recording. It features 32 recording channels, each with a 5 mm long polytrode arrangement and a 25 μm spacing between channels. The total length of the probe is 177 mm.
Automatically generated - may contain errors
4 protocols using a1 32 poly3 5mm 25s 177
1
Acute Polytrode Electrophysiology with Optogenetics
2
Acute Polytrode Electrophysiology and Respiration
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Electrophysiological signals were acquired with 32-site polytrode acute probes (A1 × 32-Poly3-5mm-25s-177, Neuronexus) through an A32-OM32 adaptor (Neuronexus) connected to a Cereplex digital headstage (Blackrock Microsystems). Unfiltered signals were digitized at 30 kHz at the headstage and recorded by a Cerebus multichannel data acquisition system (BlackRock Microsystems). Experimental events and respiration signal were acquired at 2 kHz by analog inputs of the Cerebus system. Respiration was monitored with a microbridge mass airflow sensor (Honeywell AWM3300V) positioned directly opposite the animal’s nose. Negative airflow corresponds to inhalation and negative changes in the voltage of the sensor output.
3
Multisite Electrophysiological Recordings with Respiration Monitoring
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Electrophysiological signals were acquired with a 32-site polytrode acute probe (A1 × 32-Poly3-5mm-25s-177, Neuronexus) through an A32-OM32 adaptor (Neuronexus) connected to a Cereplex digital headstage (Blackrock Microsystems). Unfiltered signals were digitized at 30 kHz at the headstage and recorded by a Cerebus multichannel data acquisition system (BlackRock Microsystems). Experimental events and respiration signal were acquired at 2 kHz by analog inputs of the Cerebus system. Respiration was monitored with a microbridge mass airflow sensor (Honeywell AWM3300V) positioned directly opposite the animal’s nose. Negative airflow corresponds to inhalation and produces negative changes in the voltage of the sensor output.
4
Polytrode Neural Signal Acquisition
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Electrophysiological signals were acquired with a 32-site polytrode acute probe (A1 × 32-Poly3-5mm-25s-177, Neuronexus, Ann Arbor, MI) through an A32-OM32 adaptor (Neuronexus) connected to a Cereplex digital headstage (Blackrock Microsystems, Salt Lake City UT). Unfiltered signals were digitized at 30 kHz at the headstage and recorded by a Cerebus multichannel data acquisition system (BlackRock Microsystems). Experimental events and respiration signal were acquired at 2 kHz by analog inputs of the Cerebus system. Respiration was monitored with a microbridge mass airflow sensor (Honeywell AWM3300V, Morris Plains, NJ) positioned directly opposite the animal’s nose. Negative airflow corresponds to inhalation and negative changes in the voltage of the sensor output.
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