For UV measurements an 8 W UV-lamp (Camag; Berlin, Germany) was used at 254 nm wavelength with a camera EOS D60 (Canon; Krefeld, Germany) for imaging in manual mode with the settings f-number F5.6, ISO1000, focal lengths 18 mm and shutter speed 1 s, carried out in a Camag UV-cabinet (Camag; Berlin, Germany). We increased the contrast between the PAN-support membrane and the PS-b-P4VP top layer by using a UV-lamp (254 nm). While the PAN backscatters parts of the UV-light, which then appears purple, PS-b-P4VP partially absorbs this wavelength and appears black. If the PS-b-P4VP layer thickness is low the UV-light will penetrate this layer, backscatter at the PAN-support membrane, and not absorb completely on its way back. In consequence, deviations of PS-b-P4VP layer thickness can be seen as purple light with changing intensity. To gain this effect, the shutter speed of the camera was increased to 1 s, which was enough for our purpose.
Eos d60
Manufactured by Canon
Sourced in Japan, Germany
The EOS D60 is a digital single-lens reflex (DSLR) camera produced by Canon. It features a 6.3-megapixel CMOS sensor and can capture images in JPEG and RAW file formats. The camera is equipped with a 3-point autofocus system and can record images at a maximum resolution of 3072 x 2048 pixels.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using eos d60
1
Visualizing Polymer Layer Thickness
2
Comprehensive Microscopic Imaging of Plant Tissues
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Whole plants were observed and photographed using an EOS D60 (Canon) or EOS Kiss X7 digital camera (Canon) and a stereoscopic microscope (SZ61; Olympus) equipped with a CCD camera (DP50, Olympus or DSX500, Olympus). Embryos were cleared using Hoyer’s solution (Feng and Ma, 2017 (link)) and observed with a BX51 upright microscope (Olympus) equipped with a CCD camera (DP72, Olympus).
For observation of leaf veins, 10-, 15-, and 20-day-old leaves were fixed in a solution (ethanol:acetate = 3:1) at room temperature. The fixed specimens were washed in an ethanol series (70%, 50%, 30%, and 15%) and rinsed with ClearSee (10% xylitol, 15% sodium deoxycholate, and 25% urea) (Kurihara et al., 2015 (link)).
To observe the cross sections of rosette leaves, leaf samples including the leaf margin were cut to a size of 2 × 2 mm with a razor. Both ends of the leaves were sliced to allow the fixative solution to permeate well into the sample, and then the samples were immersed in a fixative solution (3% glutaraldehyde, and 50 mM Na-Pi, pH 7.0) and degassed thoroughly. The samples were embedded in 5% agar and sliced to 40 μm thickness with a microtome (VT 1200 s, Leica).
For observation of leaf veins, 10-, 15-, and 20-day-old leaves were fixed in a solution (ethanol:acetate = 3:1) at room temperature. The fixed specimens were washed in an ethanol series (70%, 50%, 30%, and 15%) and rinsed with ClearSee (10% xylitol, 15% sodium deoxycholate, and 25% urea) (Kurihara et al., 2015 (link)).
To observe the cross sections of rosette leaves, leaf samples including the leaf margin were cut to a size of 2 × 2 mm with a razor. Both ends of the leaves were sliced to allow the fixative solution to permeate well into the sample, and then the samples were immersed in a fixative solution (3% glutaraldehyde, and 50 mM Na-Pi, pH 7.0) and degassed thoroughly. The samples were embedded in 5% agar and sliced to 40 μm thickness with a microtome (VT 1200 s, Leica).
3
Spectroscopy-Enabled Phase-Contrast Microscopy
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The setup for the CAAS is shown in Fig. 1a . The system is based on an inverted phase-contrast microscope (IX71, Olympus) with an integrated motorized stage, proportional integral derivative (PID; TTM-J40-R-AB, JETEC Electronics Co.)-controlled transparent heater, spectrometer (V2000, Ocean Optics) and digital camera (EOS-D60, Canon). The temperature of the biosensor chip was measured by a K-type thermocouple (TPK-02A, TECPEL) clipped between the chip and the indium tin oxide glass (ITO glass, Part No. 300739, Merck) transparent heater. Images of cells were obtained with a digital camera. The light path configuration shown in Fig. 1b allowed us to simultaneously collect spectrum signals and phase-contrast images.
4
Immunocytochemistry of Myosin Heavy Chain
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C2C12 cells were washed with phosphate buffered saline (PBS) (pH7.4) and fixed with 90% methanol at −20 °C for 10 min. After fixation, the cells were incubated in 5% milk in PBS for 30 min at 37 °C for blocking. Cells were incubated at room temperature with MF-20 (primary antibody) diluted in blocking buffer (5% milk PBS) for 1 h. After incubation, the cells were washed three times with PBS and incubated for 60 min at room temperature with a horseradish peroxidase (HRP)-conjugated α-mouse secondary antibody. The cells were washed again three times with PBS and incubated in developer (0.6 mg/mL DAB, 0.1 % H2O2 in PBS) to detect MyHC by immunocytochemistry. The nuclei were counter-stained with hematoxylin. Images were recorded with a microscope (Axiovert 35; Carl Zeiss MicroImaging) with either 4X NA 0.10 or 10X NA 0.25 Achrostigmat objective lenses with a digital camera (Canon, EOS D60; Japan).
5
Cerebral Infarct Quantification
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Brains were rapidly removed after reperfusion and mildly frozen to keep the morphology intact during slicing. Brains were sliced into 5 serial coronal sections with the aid of brain matrices. Sections were placed into 2% 2,3,5-triphenyltetra-zolium chloride (TTC) at 37°C for 10 min and then transferred into 4% paraformaldehyde for fixation at 4°C overnight. TTC-stained sections were collected by a Canon EOS D60 digital camera. The infarct area was measured using ImageJ software (NIH, Bethesda, MD, USA).
6
Glaucoma Screening Protocol: Intraocular Pressure and Fundus Analysis
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The glaucoma screening included measurements of intraocular pressure (IOP) and fundus photography. IOP was measured using a non-contact tonometer (model CT10; Topcon Inc., Tokyo, Japan). Fundus photos were taken using a 45° digital non-mydriatic fundus camera (model EOS D60; Canon Inc., Utsunomiya, Japan). Two experienced ophthalmologists (Y.K.K. and H.J.C.), masked to the subjects’ identity, independently evaluated the fundus photographs to check for suspicious findings such as glaucomatous optic neuropathy (GON) or retinal nerve fiber layer (RNFL) defects. The criteria for suspicious findings were as follows:
7
Retinal Nerve Fiber Layer and Peripapillary Atrophy Assessment
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Stereo disc photographs and red-free fundus photographs were obtained using a digital camera (EOS D60, Canon, Utsunomiyashi, Tochigiken, Japan) after dilating the pupil. A localized RNFL defect was defined on a red-free fundus photograph as a well-outlined, dark, wedge-shaped area, with the end of the dark area touching the optic disc border [11 (link)].
β-Zone PPA was defined as a region of chorioretinal atrophy adjacent to the optic disc, with visible sclera and choroidal vessels in stereo disc photographs [10 (link)]. At this step, PPA was not separated into β-zone and γ-zone PPA.
β-Zone PPA was defined as a region of chorioretinal atrophy adjacent to the optic disc, with visible sclera and choroidal vessels in stereo disc photographs [10 (link)]. At this step, PPA was not separated into β-zone and γ-zone PPA.
8
Photomacroscopic Wound Healing Analysis
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All procedures were performed in anesthetized mice. Analysis of wound healing was performed using the photomacroscopic setting of a camera (EOS D60, Canon; Krefeld, Germany) using planimetric techniques (Optimas 6.2; Meyer Instruments, Inc., Houston, TX, USA). Wound closure was considered complete when the entire surface area was epithelialized (Fig. 2). Values are given as relative decrease of wound area in relation to the initial value.
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