TRAP staining of tissue sections and cells were performed using the TRAP kit (Wako), and multinucleated TRAP-positive cells were quantified.
Trap kit
Manufactured by Fujifilm
The TRAP kit is a laboratory equipment used for the detection and analysis of targeted RNA-binding proteins (RBPs). It provides a standardized procedure for the isolation and purification of RBP-RNA complexes from cell or tissue samples. The kit includes reagents and protocols for cell lysis, RNA-protein complex capture, and downstream analysis.
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Lab products found in correlation
2 protocols using trap kit
1
Quantifying Multinucleated TRAP-Positive Cells
2
Cryosectioning and TRAP Staining of Murine Tibiae
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tibiae of 10% ethanol‐treated or water‐treated mice were also separated for TRAP staining. The samples were fixed in 4% paraformaldehyde at 4°C for 48 h, followed by immersion fixation in 30% sucrose at 4°C for 48 h. Next, the samples were placed in the Tissue‐Tek O.C.T. compound (SAKURA) at −80°C until staining. The cryostat section (8 μm) was obtained by Kawamoto's film method (SECTION‐LAB, Co., Ltd.)33 and stained using the TRAP Kit (FUJIFILM Wako Pure Chemical Corporation) according to the manufacturer's protocol.
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