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2 protocols using α tublin

1

Comprehensive Protein Expression Analysis

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AR(rabbit monoclonal, 1:1000, #5153), PSA/KLK3(rabbit monoclonal, 1:1000, #5365), TOMM20(rabbit monoclonal, 1:1000, #42,406), ATG5(rabbit monoclonal, 1:1000, #12994S), LC3I/II(rabbit monoclonal, 1:1000, #12,741), Akt (rabbit monoclonal, 1:1000, #4691), Phospho-Akt (Ser473)(rabbit monoclonal, 1:1000, #4060), Phospho-Akt (Thr308)(rabbit monoclonal, 1:1000, #13,038), Nanog(rabbit monoclonal, 1:1000, #4903), Sox2(rabbit monoclonal, 1:1000, #3579), ALDH1A1(rabbit monoclonal, 1:1000, #36671S) and VDAC(rabbit monoclonal, 1:1000, #4661) antibodies were purchased from Cell Signaling Technology (CST). NCAM1(rabbit polyclonal antibody, 1:1000, A7913), β-Actin(mouse polyclonal, 1:5000, AC004) and α-Tublin(mouse polyclonal, 1:5000, AC012) antibodies were purchased from ABclonal Technology (Upper Heyford, UK). NSE (rabbit monoclonal, 1:1000, ab180943) and Syp (Rabbit monoclonal, 1:1000, ab184176) were purchased from Abcam (Shanghai, China). ALDH1A1(mouse monoclonal antibody, 1:500,sc-166362), TOMM70(mouse monoclonal antibody, 1:500, sc-390545),AR(rabbit polyclonal, 1:1000, Sc-815X) antibodies were purchased from Santa cruz. E-cadherin (rabbit polyclonal, 1:1000, GTX100443),BRN2(rabbit polyclonal, 1:1000, GTX114650) and N-cadherin (rabbit polyclonal, 1:1000, GTX127345) were purchased from Genetex.
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2

Antibody Profiling for Cellular Processes

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The primary antibodies used in this study are listed as follows: GAPDH (Abclonal, AC002), CapZβ (Proteintech, 25,043–1-AP), His6-tag (ProteinTech, 66,005–1-Ig), α-tublin (Abclonal, AC012), RAB5A (CST, 3547), LAMP1 (CST, 9091), Rab7 (Abcam, ab137029), EEA1 (CST, 3288), Myc-tag (ProteinTech, 67,447–1-Ig), Flag-tag (Abclonal, AE005), HA-tag (Proteintech, 66,006–2-Ig), EGFR (Novusbio, AF231), NSP9 (GeneTex, GTX636839), Zikv-E (GeneTex, GTX133314), c-PARP (CST, 5625), DsRNA (Abcam, ab288755), goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, 31,430), goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, 31,460).
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