1h mrs

Manufactured by Echo Medical Systems

Sourced in United States

1H-MRS is a non-invasive magnetic resonance spectroscopy technique that measures the concentration of specific metabolites in the human body. It provides quantitative information about the chemical composition of tissues, with a focus on proton (1H) signals. This equipment allows for the analysis of various metabolites, enabling researchers and clinicians to gain insights into biochemical processes and tissue function.

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Lab products found in correlation

Metabolic cages, by TSE Systems (2 mentions) C57bl 6j mice, by Jackson ImmunoResearch (2 mentions) Cryoprep impactor, by Covaris (1 mentions) S3282, by Bio-Serv (1 mentions) B6 cg speer6 ps1tg alb cre 21mgn j mice, by Jackson ImmunoResearch (1 mentions) B6 fvb tg adipoq cre 1evdr mice, by Jackson ImmunoResearch (1 mentions) C56bl 6j mice, by Jackson ImmunoResearch (1 mentions) B6 cg tg alb cre 21mgn j mice, by Jackson ImmunoResearch (1 mentions)

9 protocols using 1h mrs

Whole-body Composition Assessment by 1H-MRS

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Whole-body composition was assessed by ¹H-MRS (Echo Medical Systems).

Al-Share Q.Y., DeAngelis A.M., Lester S.G., Bowman T.A., Ramakrishnan S.K., Abdallah S.L., Russo L., Patel P.R., Kaw M.K., Raphael C.K., Kim A.J., Heinrich G., Lee A.D., Kim J.K., Kulkarni R.N., Philbrick W.M, & Najjar S.M. (2015). Forced Hepatic Overexpression of CEACAM1 Curtails Diet-Induced Insulin Resistance. Diabetes, 64(8), 2780-2790.

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Noninvasive Metabolic Profiling of Genetically Modified Mice

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Whole-body fat and lean mass were noninvasively measured in 16 weeks old control flox, AdFASNKO and AdACC1KO female mice using ¹H-MRS (Echo Medical Systems, Houston, TX). Energy balance was noninvasively measured using metabolic cages (TSE Systems, Chesterfield, MO) and indirect calorimetry to assess food and water intake, energy expenditure, respiratory exchange ratio, and physical activity for 3 days.^{81 (link)} The CalR app^{67 (link),82 (link)} was used for the statistical analysis and data presentation of the metabolic cage studies.

Leach T.J., Chotkowski H.L., Wotring M.G., Dilwith R.L, & Glaser R.L. (2000). Replication of Heterochromatin and Structure of Polytene Chromosomes. Molecular and Cellular Biology, 20(17), 6308-6316.

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Whole Body Composition Analysis in Mice

Cited 1 time

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Whole body composition was assessed in awake mice by NMR technology (Bruker Minispec, Billerica, MA, USA). Magnetic resonance spectroscopy (¹H-MRS; Echo Medical Systems, Houston, TX, USA) was used to determine fat and lean mass as percentage of total body weight, as previously described [15 (link)].

Russo L., Muturi H.T., Ghadieh H.E., Ghanem S.S., Bowman T.A., Noh H.L., Dagdeviren S., Dogbey G.Y., Kim J.K., Heinrich G, & Najjar S.M. (2017). Liver-specific reconstitution of CEACAM1 reverses the metabolic abnormalities caused by its global deletion in male mice. Diabetologia, 60(12), 2463-2474.

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High-Fat Diet Induces Liver Metabolic Changes

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We obtained male C57BL/6J mice (stock number 000664) from the Jackson Laboratory. All mice were housed in a specific pathogen-free facility accredited by the American Association for Laboratory Animal Care. We fed the mice either (1) a standard CD (Prolab Isopro RMH 3000, Purina) for 24 weeks or (2) 8 weeks of standard CD followed by 16 weeks of HFD (S3282, Bio-serve). We measured fat and lean mass noninvasively using ¹H-MRS (Echo Medical Systems). We euthanized all mice at 24 weeks after an overnight fast and froze the livers prior to removal using clamps cooled in liquid nitrogen. The frozen livers were then pulverized into a powder using a CryoPREP impactor (Covaris). We prepared aliquots of pulverized liver for all samples for subsequent analyses. All experiments were carried out in accordance with guidelines for the use of laboratory animals and were approved by the Institutional Animal Care and Use Committees (IACUCs) of the University of Massachusetts Medical School.

Soltis A.R., Kennedy N.J., Xin X., Zhou F., Ficarro S.B., Yap Y.S., Matthews B.J., Lauffenburger D.A., White F.M., Marto J.A., Davis R.J, & Fraenkel E. (2017). Hepatic Dysfunction Caused by Consumption of a High-Fat Diet. Cell reports, 21(11), 3317-3328.

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In vivo Hyperglycemic Clamp for Metabolic Phenotyping

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In vivo hyperglycemic clamp experiments were performed at the National Mouse Metabolic Phenotyping Center (MMPC) at UMass Medical School. Body composition analysis was conducted by noninvasively measuring whole body fat mass and lean mass using ¹H-MRS (Echo Medical Systems, Houston, TX). A survival surgery was performed at 5–6 days before hyperglycemic clamp experiments to establish an indwelling catheter in the jugular vein. On the day of experiment, mice were fasted overnight (~15 h), and a 2 h hyperglycemic clamp was conducted in awake mice by intravenously infusing 20% dextrose to rapidly raise and maintain plasma glucose levels at ~19 mM^{95 (link)}. Blood samples were taken at 10–20 min intervals to measure plasma insulin levels during hyperglycemic clamps.

Skovsø S., Panzhinskiy E., Kolic J., Cen H.H., Dionne D.A., Dai X.Q., Sharma R.B., Elghazi L., Ellis C.E., Faulkner K., Marcil S.A., Overby P., Noursadeghi N., Hutchinson D., Hu X., Li H., Modi H., Wildi J.S., Botezelli J.D., Noh H.L., Suk S., Gablaski B., Bautista A., Kim R., Cras-Méneur C., Flibotte S., Sinha S., Luciani D.S., Nislow C., Rideout E.J., Cytrynbaum E.N., Kim J.K., Bernal-Mizrachi E., Alonso L.C., MacDonald P.E, & Johnson J.D. (2022). Beta-cell specific Insr deletion promotes insulin hypersecretion and improves glucose tolerance prior to global insulin resistance. Nature Communications, 13, 735.

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Noninvasive Metabolic Profiling of Genetically Modified Mice

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Guilherme A., Rowland L.A., Wetoska N., Tsagkaraki E., Santos K.B., Bedard A.H., Henriques F., Kelly M., Munroe S., Pedersen D.J., Ilkayeva O.R., Koves T.R., Tauer L., Pan M., Han X., Kim J.K., Newgard C.B., Muoio D.M, & Czech M.P. (2023). Acetyl-CoA carboxylase 1 is a suppressor of the adipocyte thermogenic program. Cell reports, 42(5), 112488.

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Metabolic Analysis of Genetically Modified Mice

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C57BL/6J mice (RRID: IMSR_JAX:000664), B6;FVB-Tg(Adipoq-Cre)1Evdr mice (RRID: IMSR_JAX:028020) (Eguchi et al. 2011 (link)), and B6.Cg-Speer6-ps1^{Tg(alb-cre)21Mgn}/J mice (RRID: IMSR_ JAX:003574) (Postic et al. 1999 (link)) were obtained from The Jackson Laboratory. We have previously described Adipoq^−/− mice (Nawrocki et al. 2006 (link)), Fgf21^LoxP/LoxP mice (Vernia et al. 2016a (link)), Mapk8^LoxP/LoxP mice (Das et al. 2007 (link)), and Mapk9^LoxP/LoxP mice (Han et al. 2013 (link)). The mice were backcrossed to the C57BL/6J strain (10 generations) and housed in a specific pathogen-free facility accredited by the American Association for Laboratory Animal Care using laminar flow cages at 21°C. Mice were housed at thermoneutrality (30°C) in the vivarium using rodent incubators (Powers Scientific, Inc. model RIT33SD). Cold exposure was performed by reducing the temperature 2°C/day from ambient to the maintenance temperature (6°C) using rodent incubators in the vivarium. Male mice (age 8 wk) were fed a chow diet (Purina IsoPro 3000) or a high-fat diet (Bio-Serv S3282) (Han et al. 2013 (link)). Whole-body fat and lean mass were noninvasively measured by ¹H-MRS (Echo Medical Systems). The studies were approved by the Animal Care and Use Committees of the University of Massachusetts Medical School, Yale Medical School, and the University of Cincinnati.

Han M.S., Perry R.J., Camporez J.P., Scherer P.E., Shulman G.I., Gao G, & Davis R.J. (2021). A feed-forward regulatory loop in adipose tissue promotes signaling by the hepatokine FGF21. Genes & Development, 35(1-2), 133-146.

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Conditional Knockout Mice for Jnk1 and Jnk2

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We have previously described Jnk1^LoxP/LoxP mice (Das et al., 2007 (link)) and Jnk2^LoxP/LoxP mice (Han et al., 2013 (link)). C56BL/6J mice (stock number 000664), and B6.Cg-Tg(Alb-cre)21Mgn/J mice (stock number 003574) (Postic et al., 1999 (link)) were obtained from The Jackson Laboratory. The mice were backcrossed to the C57BL/ 6J strain (ten generations) and housed in a facility accredited by the American Association for Laboratory Animal Care (AALAC). The mice employed in this study were L^WT (Cre⁺), L^Δ1 (Cre⁺ Jnk1^LoxP/LoxP), L^Δ2 (Cre⁺ Jnk2^LoxP/LoxP), and L^Δ1,2 (Cre⁺ Jnk1^LoxP/LoxP Jnk2^LoxP/LoxP). All studies were performed using male mice (8–24 wk old). Male mice (8 weeks old) were fed a standard Control (chow) diet or a high fat diet (HFD) (Iso Pro 3000, Purina and F3282, Bioserve, Inc.). Whole body fat and lean mass were non-invasively measured using ¹H-MRS (Echo Medical Systems). The animal studies were approved by the Institutional Animal Care and Use Committee of the University of Massachusetts Medical School.

Vernia S., Cavanagh-Kyros J., Garcia-Haro L., Sabio G., Barrett T., Jung D.Y., Kim J.K., Xu J., Shulha H.P., Garber M., Gao G, & Davis R.J. (2014). The PPARα - FGF21 hormone axis contributes to metabolic regulation by the hepatic JNK signaling pathway. Cell metabolism, 20(3), 512-525.

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Non-invasive Mouse Body Composition

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Fat mass and lean mass were measured by the University of Massachusetts Mouse Metabolic Phenotyping Center. Conscious mice were non-invasively assessed for fat and lean mass using 1H-MRS (Echo Medical Systems, Houston, TX).

Huynh F.K., Hu X., Lin Z., Johnson J.D, & Hirschey M.D. (2017). Loss of sirtuin 4 leads to elevated glucose- and leucine-stimulated insulin levels and accelerated age-induced insulin resistance in multiple murine genetic backgrounds. Journal of inherited metabolic disease, 41(1), 59-72.

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