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Abi 3130xl dna sequence detector

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The ABI 3130XL DNA sequence detector is a capillary electrophoresis-based genetic analysis instrument. It is designed to perform DNA sequencing and fragment analysis.

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Lab products found in correlation

Genemapper 4, by Thermo Fisher Scientific (4 mentions) Snapshot multiplex kit, by Thermo Fisher Scientific (4 mentions) Dna purification kit, by Promega (3 mentions)

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ABI 3130XL (189 citations)

4 protocols using abi 3130xl dna sequence detector

1

SNaPshot Genotyping Protocol

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Genomic DNA was extracted and the SNaPshot reaction was performed for genotyping as previously described (Wang et al., 2016, 2017) using the SNaPshot Multiplex Kit (Applied Biosystems Co., Ltd., Foster City, CA, USA). Table 1 shows the sequences of the PCR primers. Experimental results were analyzed using an ABI 3130XL DNA sequence detector and GeneMapper 4.0 (Applied Biosystems Co., Ltd.).
Wang Y.Z., Zhang H.Y., Liu F., Li L., Deng S.M, & He Z.Y. (2019). Association between PPARG genetic polymorphisms and ischemic stroke risk in a northern Chinese Han population: a case-control study. Neural Regeneration Research, 14(11), 1986-1993.
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2

Genotyping of let-7f-2 Regulatory SNP

Cited 2 times
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The University of California, Santa Cruz (UCSC) genome browser1 and the SNP database (dbSNP)2 were used to find the relevant SNPs. The SNPs were included if they were situated −1 kb upstream of the pri-let-7f-2 on the X chromosome, had a minor allele frequency above 0.1 in the Han Chinese population, and were identified as possible functional SNPs by the National Institute of Environmental Health Sciences’ SNP function prediction (FuncPred) program.3 One relevant SNP, rs17276588, was discovered at −183 bp upstream of the pri-let-7f-2 on the X chromosome (Table 1).
The DNA purification kit Promega (Madison, WI, USA), was used to extract genomic DNA from the peripheral blood, and the SNaPshot reaction was done as previously described (8 (link)–10 (link)). The genotyping analysis was done using the SNaPshot Multiplex kit (Applied Biosystems Co., Ltd., Foster City, CA, USA). The ABI 3130XL DNA sequence detector and GeneMapper 4.0 were used to analyze the data (Applied Biosystems Co., Ltd.).
Wang Y., Qiu L., Wang Y., He Z., Lan X., Cui L, & Wang Y. (2022). Genetic variation within the pri-let-7f-2 in the X chromosome predicting stroke risk in a Chinese Han population from Liaoning, China: From a case-control study to a new predictive nomogram. Frontiers in Medicine, 9, 936249.
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3

Genotyping Analysis of Blood Samples

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Genomic DNA was extracted from 200 µL of ethylenediaminetetraacetic acid-anticoagulated peripheral blood using a DNA Purification Kit (Promega, Madison, WI, USA), and a SNaPshot reaction was performed, as previously described (Wang et al., 2016a, 2017). A SNaPshot Multiplex Kit (Applied Biosystems Co., Ltd., Foster City, CA, USA) was used for the genotyping analysis. Table 1 shows the sequences of the polymerase chain reaction primers used in this study. The experimental results were analyzed on an ABI 3130XL DNA sequence detector and in GeneMapper 4.0 (Applied Biosystems Co., Ltd.).
Wang Y.Y., Zhang H.Y., Jiang W.J., Liu F., Li L., Deng S.M., He Z.Y, & Wang Y.Z. (2020). Genetic polymorphisms in pri-let-7a-2 are associated with ischemic stroke risk in a Chinese Han population from Liaoning, China: a case-control study. Neural Regeneration Research, 16(7), 1302-1307.
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4

Genomic DNA Extraction and Genotyping

Cited 2 times
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Genomic DNA was extracted from ethylenediaminetetraacetic acid-anticoagulated peripheral blood and stored at −80°C. The SNaPshot reaction was performed, as described previously.17 (link),18 (link) DNA was extracted using a DNA Purification Kit (Promega, Madison, WI, USA) and genotype analysis was carried out using a SNaPshot Multiplex Kit (Applied Biosystems Co., Ltd., Foster City, CA, USA). The primer sequences used for polymerase chain reaction are shown in Table 2. The results were analyzed using an ABI 3130XL DNA sequence detector and GeneMapper 4.0 (Applied Biosystems Co., Ltd.).
Qiu L., Wang Y., Liu F., Deng S., He Z., Zheng W, & Wang Y. (2023). Genetic polymorphisms of pri-let-7f, gene–environment and gene–gene interactions, and associations with ischemic stroke risk in Liaoning Province. The Journal of International Medical Research, 51(5), 03000605231173578.
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