Dsc 822

Differential Scanning Calorimetry (DSC) analysis of 3D-printed collagen gels (Col and Col-Ag) were performed using a DSC Calorimeter (DSC 822, Mettler Toledo, Switzerland). For this purpose, a known amount (5–10 mg) of each sample was placed in an aluminum crucible and the DSC curves were obtained with a heat flow of 10 °C min⁻¹ from 25 to 80 °C, under a nitrogen atmosphere. The collagen denaturation temperature was determined at the maximum of the melting peak after baseline subtraction.

The calorimetric thermograms were obtained by scanning with a differential scanning calorimeter Mettler DSC-822 in flowing N₂ atmosphere and heating and cooling rates of 10 °C/min.
Wide angle X-ray scattering measurements (WAXS) were obtained with Ni-filtered Cu Kα radiation with a Philips automatic diffractometer. Both neat polymer samples and derived loaded NPs were dehydrated under N₂ flux before the analyses.

The thermal properties of native and hydrothermally modified starches were measured using differential scanning calorimetry (DSC822, Mettler Toledo, Switzerland) according to the method of Man et al. [8 (link)]. Starch sample was precisely weighed (2.5 mg, d.b.) and mixed with 3 times (by weight) deionized-distilled water (total weight = 10 mg). The mixture was hermetically sealed in an aluminum pan and kept overnight at 4 °C for moisture equilibration, then equilibrating for 1 h at room temperature prior to thermal analysis. Thermal properties of starch sample were measured using a differential scanning calorimetry by heating from 25 to 110 °C at a rate of 10 °C/min. The onset, peak and concluding temperature, as well as enthalpy during phase transition were then determined.

The physical form of the drug in the microspheres was first analyzed using differential scanning calorimetry (DSC; DSC822 instrument, Mettler Toledo, Columbus, OH, USA). Accurately weighed samples (ca. 3–5 mg) were placed in aluminum pans and sealed. The samples were then heated at a constant rate of 2 °C/min from 0 to 100 °C under a dry atmosphere of nitrogen.
X-ray powder diffraction (XRD) patterns of the samples were recorded on a D/MAX-2500PC instrument (Rigaku, Tokyo, Japan) supplied with Cu Kα radiation. Diffraction patterns were collected over the 2θ range 10–75° with a step size of 0.01° (2θ) and count time of 1 s per step.

DSC was performed using a Mettler Toledo DSC 822 on the solid, as-prepared peptides with 80 cm³/min N₂ and a heating rate of 5 °C/min from 20 °C to 70 °C while the power required to maintain this rate was monitored.