The capacity of BgElo12 and BgElo24 to contribute to water retention was assessed by a desiccation bioassay. Drying bottles were prepared by putting ~120 g of packed fresh silica gel into a ~900 mL sealed plastic bottle. The RH inside the bottle dropped to 5% within 2 h which was monitored by HOBO Pro v2 (Onset, Bourne, USA). AD2 females were injected with dsBgElo12, dsBgELo24, and dsMuslta and separately caged in the desiccation bottles at 30℃, supplied with ~1 g of dry food, but no water. Survival was recorded every 8 h until all the cockroaches died. About 100 cockroaches were used for each treatment.
Pro v2
Manufactured by Hobo
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The HOBO Pro v2 is a compact, self-contained data logger designed to record environmental data. It features a range of sensors to measure temperature, relative humidity, and other parameters. The HOBO Pro v2 is a versatile data logging device suitable for a variety of monitoring applications.
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12 protocols using pro v2
1
Desiccation Resistance of BgElo Knockdown
2
Freeze-Thaw Cycle Effects on Plant Ecotypes
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The FTC manipulations were administered in a three-factorial design, manipulating: (i) FTC frequency (zero for control plants and one, three and seven cycles each for the respective treatment trays), (ii) different minimum temperatures (4.5 °C for the control and −4 and −8 °C for each freezing treatment) and (iii) plant ecotypes (12 ecotypes). Each factorial combination was replicated five times, adding up to 420 plants in total.
Freeze-thaw cycle manipulations lasted a total of 7 days (Fig. 2 ). In order to simulate natural conditions, plants experienced thawing at 4.5 °C during the day and night-time freezing down to −4 or −8 °C in separate climate chambers (Fig. 2 ). For treatment, plants were moved from the control chamber (4.5 °C) to one of two treatment chambers set at either −4 or −8 °C. The temperature inside the chambers was measured using temperature loggers (HOBO Pro v2, Onset Computer Corporation, MA, USA). The last freezing cycle overlapped with the last day of treatment to affect all plants equally. Following the freezing treatments, plants were transferred into the greenhouse for a growth period of 2 weeks at an average mean air temperature of 20 °C and 43 % mean relative humidity during the day in order to simulate natural spring conditions.
Freeze-thaw cycle manipulations lasted a total of 7 days (
3
Desiccation Tolerance Bioassay for Cockroaches
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The capacity of BgElo12 and BgElo24 to contribute to water retention was assessed by a desiccation bioassay. Drying bottles were prepared by putting approximately 120 g of packed fresh silica gel into an approximately 900-mL sealed plastic bottle. The RH inside the bottle dropped to 5% within 2 hours, which was monitored by HOBO Pro v2 (Onset, Bourne, Massachusetts, USA). AD2 females were injected with dsBgElo12, dsBgELo24, and dsMuslta and separately caged in the desiccation bottles at 30°C, supplied with approximately 1 g of dry food, but no water. Survival was recorded every 8 hours until all the cockroaches died. About 100 cockroaches were used for each treatment.
4
Equine Rectal Temperature Measurement
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A temperature data logger (HOBO Pro v2; U23-002; Onset Computer Corporation; Bourne, MA, USA) was used to obtain rectal temperature (TR) during exercise. A 184 cm long thermal sensor was fed through a universal insemination pipette for mares and introduced 40 to 50 cm into the rectum. Fecal content was removed prior to insertion of the thermal sensor. The data logger was secured to the tail using vet wrap.
5
Environmental Parameters at Ca. Nitrotoga sp. CP45 Site
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Environmental parameters were measured at the river site where the Ca. Nitrotoga sp. CP45 culture inoculum was collected (see above). Briefly, surface water parameters were measured approximately every 4–8 weeks from May 2015 to July 2016. Temperature and pH were measured with a YSI Professional Plus handheld multiparameter meter (YSI Incorporated, Yellow Springs, Ohio).
Water quality data were obtained from the Northern Water Conservancy District (Northern Water Conservancy District, 2016 ) at a site 2 miles downstream from the CP45 sampling site (latitude 40.4244, longitude −104.6000). Data included water temperature, pH, turbidity, and dissolved inorganic nitrogen concentrations. Samples were obtained monthly from April of 2015 through November of 2016 (with the exception of January and December), analyzed at a USGS certified laboratory, and approved in a QA/QC process. At a sampling site 9 miles upstream from CP45 (latitude 40.5013, longitude −104.9673), water temperature was obtained at 15-min intervals around-the-clock from April of 2015 through May of 2016 using a HOBO ProV2 temperature logger (Onset Computer Corporation, Bourne, MA).
Water quality data were obtained from the Northern Water Conservancy District (Northern Water Conservancy District, 2016 ) at a site 2 miles downstream from the CP45 sampling site (latitude 40.4244, longitude −104.6000). Data included water temperature, pH, turbidity, and dissolved inorganic nitrogen concentrations. Samples were obtained monthly from April of 2015 through November of 2016 (with the exception of January and December), analyzed at a USGS certified laboratory, and approved in a QA/QC process. At a sampling site 9 miles upstream from CP45 (latitude 40.5013, longitude −104.9673), water temperature was obtained at 15-min intervals around-the-clock from April of 2015 through May of 2016 using a HOBO ProV2 temperature logger (Onset Computer Corporation, Bourne, MA).
6
Soil Sampling and Environmental Monitoring Protocol
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After cutting the stems to ground level, we collected soil core samples from three random locations in each subplot with a cylindrical soil auger (5 cm inner diameter, 15 cm length). We combined the three replicates from the same depth for each subplot as a single composite sample, dried it in the shade, and filtered it using a 2‐mm sieve for stoichiometric analysis. We measured soil pH, concentration of nitrogen (N), phosphorus (P), and carbon (C) following the standard protocols (Sparks et al., 1996 ). Besides soil resources, we also collected climatic data for rainfall, air temperature, and air moisture using HOBO RG3‐M, HOBO Pro v2, respectively (Onset Computer Corporation, Bourne, MA, USA) from July to October in 2016. For each elevation, we placed one HOBO RG3‐M and two HOBO Pro v2, of which one was positioned inside an open top chamber and the other one was positioned in a control plot. We showed the detailed distributions of temperature and moisture during the experimental interval in Figure A1 .
7
Underwater Temperature Monitoring
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A temperature logger (HOBO Pro v2, Onset Computer Corporation, USA) was attached to a rope and set to measure at every 5 m depth from surface to 40 m depth on each sampling day from January 2013 to July 2014. In addition, temperature loggers were set to measure hourly and deployed at the sampling site (40 m depth) from April 2014 to August 2016 and at 1–2 m depth on the reef flat in front of the station from January 2013 to August 2016.
8
Tracking Stream Temperature and Discharge
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The authors collected water temperature and discharge data for each stream throughout the study period to determine their influence on movement and habitat-use patterns. They placed temperature loggers (HOBO Pro v2, Onset Corp., Bourne, MA, USA) in two or three equally spaced, well-mixed locations (c. 0.75 m deep) along the thalweg in each stream and recorded temperature every 30 min. The authors averaged temperature data across loggers within streams to calculate mean daily temperatures for comparison with fish movements. Mean daily discharge data were compiled from U.S. Geological Survey (USGS) stream gages on Buffalo Creek (gage 07189100) and Elk River (gage 07189000) near Tiff City, Missouri. The authors characterized Sycamore Creek discharge patterns by developing a rating curve. Briefly, the authors deployed a water level logger (HOBO U20, Onset Corp.) to continuously record stream stage, and compared stage values to measured cross-sectional discharge (Gordon et al., 2004) :
where Q is the discharge (m 3 s À1 ), S is the stage height (m) and a and b are system-specific coefficients. The rating curve coefficients for Sycamore Creek were a = 5.11 and b = 6.66, and the relationship had an R 2 = 0.63. Gaps in discharge data were estimated using leastsquares regression with discharge values from the USGS gage on nearby Honey Creek (gage 07189542; R 2 = 0.94).
where Q is the discharge (m 3 s À1 ), S is the stage height (m) and a and b are system-specific coefficients. The rating curve coefficients for Sycamore Creek were a = 5.11 and b = 6.66, and the relationship had an R 2 = 0.63. Gaps in discharge data were estimated using leastsquares regression with discharge values from the USGS gage on nearby Honey Creek (gage 07189542; R 2 = 0.94).
9
Seawater Temperature Monitoring in Mo'orea
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In situ seawater temperature was recorded with loggers (Hobo Pro v2, ± 0.2°C, Onset Computer Corp., Bourne, MA) at ≈ 1.5-m depth, ≈ 100 m behind the reef crest (Site B*, Fig 1 ). Loggers recorded at 0.002 Hz and were installed in September 2005, and exchanged in approximately January and August of each year. Temperature records were averaged by day, and daily values were used to characterize each year, although not all months were sampled due to equipment losses. The thermal environment of seawater on the north shore of Mo’orea was described by the mean and 95% confidence interval (CI) by day between 2005 and 2015. In situ daily seawater temperature in 2016 was interpreted relative to this record. A broader perspective of seawater temperature was provided through NOAA CRW for the “Society Archipelago”, which is a Regional Virtual Station represented by a 5 × 5 km cell centered on 151.375°E 16.950S (https://coralreefwatch.noaa.gov/ accessed 29 August 2017). These data describe nighttime ocean temperature at the surface, and here the 90th percentile hotspot values are reported for the station, together with the degree heating weeks (DHW) calculated from them.
10
Mosquito Surveillance in Long Island, NY
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Mosquitoes were collected in Long Island, New York, USA, at four farms and four residential areas with 9–17 houses in each, totaling 50 properties. Sites were chosen based on prior knowledge of Ae. albopictus distribution in Suffolk County from larval surveys and vector control surveillance [40 ] (S. Campbell, pers comm). The eight sites were located in separate towns spanning a substantial section of Long Island (40km East to West and 15km North to South) (Fig 1 ). All four farms were surrounded to some degree by both forested and residential land. The four residential areas had variable levels of vegetation, both within and between sites. Residential property sizes ranged from approximately 200 – 1200m2. Terrain was flat across all 8 sites (elevation range approximately 3–79m above sea level). Collections were conducted between June and August 2018. Two HOBO Pro v2 data loggers (model U23-001, Onset Computer Corp., Bourne MA, USA) per site recorded the temperature and humidity every four hours from mid-July through August.
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