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Il 6 quantikine hs elisa kit

Manufactured by R&D Systems
Sourced in United States

The IL-6 Quantikine HS ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of interleukin 6 (IL-6) levels in cell culture supernates, serum, and plasma.

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3 protocols using il 6 quantikine hs elisa kit

1

Biochemical Markers of Exercise-Induced Muscle Damage and Oxidative Stress

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CK is a biomarker of muscle damage and usually increases after strenuous exercise 30 . In our study, CK levels were determined with an autoanalyzer (Hitachi 7060, Tokyo, Japan) from blood samples obtained before and 3, 24, and 48 hours after the exhaustive exercise regimen.
Exercise training would generate oxidative stress and regulate the endogenous antioxidant defense system (including GPx) 31 . The analysis of plasma collected pre-exercise and 3 and 48 h post exercise was performed with the GPx-Assay-Kit (Cayman Chemical Company, Ann Arbor, Michigan, USA).
IL-6 is a multifunctional cytokine involved in pro-inflammatory and anti-inflammatory processes 32 (link). The IL-6 response caused by exercise depends on the intensity and duration of the exercise. The analysis of serum collected pre-exercise and 3 and 48 h post exercise was performed with the IL-6 Quantikine HS ELISA Kit (R&D Systems, Inc. Minneapolis, MN, USA).
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2

Cardiometabolic Health Evaluation by Serum Studies

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Serum studies were used to evaluate cardiometabolic health indicators and inflammation. All participants underwent a 12-hour fasting blood draw at the clinical research unit where a trained phlebotomist drew approximately 20 mL of blood. Serum measures of HgA1c, glucose, insulin, and hsCRP were analyzed fresh samples using standard clinical procedures and commercially available assays at the hospitals lab. We used participants’ fasting glucose and insulin measures to calculate the homeostatic model assessment of insulin resistance (HOMA-IR) for each individual.32 (link) IL-6 levels were measured in batch from plasma stored at −80 degrees C using the IL-6 Quantikine HS ELISA Kit from R&D Systems, INC (Minneapolis, MN). All assays were conducted according to the manufactures’ instructions.
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3

Cardiometabolic and Inflammatory Markers

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Serum studies were used to evaluate cardiometabolic health indicators and inflammation. All participants underwent a 12-hour fasting blood draw at the clinical research unit where a trained phlebotomist drew approximately 20 mL of blood. Serum measures of glucose, insulin, high-sensitivity C-Reactive protein, and cholesterol were analyzed from fresh samples using standard clinical procedures and commercially available assays at the hospitals lab. We used the participants’ fasting glucose and insulin measures to calculate the Quantitative Insulin-Sensitivity Check Index (QUICKI) for each individual. Interleukin 6 levels were measured in batch from plasma stored at −80 degrees centigrade using the IL-6 Quantikine HS ELISA Kit (R&D Systems, INC, Minneapolis, MN). All assays were conducted according to the manufactures’ instructions.
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