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Haecs

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Human Aortic Endothelial Cells (HAECs) are primary cells isolated from human aortic tissue. They are intended for use in cell culture research.

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Lab products found in correlation

Endothelial cell medium (ecm), by ScienCell (5 mentions) Haecs, by Merck Group (2 mentions) Snail sirna, by GenePharma (1 mentions) Fibronectin from bovine plasma, by Merck Group (1 mentions) Endothelial cell growth medium, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Endothelial cell growth supplement (ecgs), by BD (1 mentions) Endothelial cell growth factor, by ScienCell (1 mentions) Penicillin streptomycin, by ScienCell (1 mentions) Penicillin, by ScienCell (1 mentions) Streptomycin, by ScienCell (1 mentions) Haecs, by RiboBio (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions) Hasmc, by Thermo Fisher Scientific (1 mentions) Hasmc, by Lonza (1 mentions) Complete ec medium, by ScienCell (1 mentions) Epidermal growth factor (egf), by PromoCell (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by PromoCell (1 mentions)

21 protocols using haecs

1

Nicotine-induced Endothelial Dysfunction

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Human aortic endothelial cells (HAECs) were purchased from ScienCell Research Laboratories (Catalog #6100, Carlsbad, CA, USA) and were cultured in endothelial cell medium (pH 7.4) supplemented with 5% FBS, 1% endothelial cell growth factors, and 1% penicillin/streptomycin. The cells were maintained in 5% CO2 and 95% air at 37°C. Average serum nicotine level in moderate smokers is 220 nmol/L and the level can reach 440 nmol/L after consumption of a single cigarette 28 (link). Therefore, HAECs were incubated with 50 or 500 nM nicotine for 48 h in our experiments. α-BTX (1 μM) was used to block α7nAChR in cell experiments. PD98059 (20 μM) was added 1 h before nicotine treatment in the corresponding experiments. For transfection experiments, Snail siRNA obtained from GenePharma Co., Ltd (Suzhou, China) was used to knockdown Snail in HAECs. After 24 h of transfection, the medium was replaced by fresh medium with or without nicotine. After drug treatment, the cells were used for cell morphology observation, immunofluorescent staining and protein/RNA extraction.
Qin W., Zhang L., Li Z., Xiao D., Zhang Y., Zhang H., Mokembo J.N., Monayo S.M., Jha N.K., Kopylov P., Shchekochikhin D, & Zhang Y. (2020). Endothelial to mesenchymal transition contributes to nicotine-induced atherosclerosis. Theranostics, 10(12), 5276-5289.
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2

Investigating Endothelial Cell Responses to Shear Stress

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Human Aortic Endothelial cells (HAECs)(Sciencell, San Diego, CA) and Human Aortic Valve Endothelial cells (HAVECs) were placed on plates coated with 10 μg/mL fibronectin from bovine plasma (Sigma, St Louis, MO) and grown in endothelial cell media (ECM)(Sciencell) at 5% CO2. Media was changed every other day and cells were passaged with 0.05% Trypsin EDTA when confluent. All experiments were performed with cells with fewer than 8 passages. Cells were cultured in unidirectional pulsatile shear stress conditions using μSlide I 0.8 Luer channel slides and flow kit (Ibidi, Verona, WI) and a L/S® Modular brushless digital dispensing drive peristaltic pump with 6-roller cartridge pump head (Cole Parmer, Vernon Hills, IL). Cells in shear stress conditions modeling laminar flow were exposed to media flow at 15 dynes/cm2 and cells in the static condition were grown in well plates. While only one flow rate was used, multiple flow rate conditions (i.e., low vs. high) may uncover even more gene expression differences.
White M.P., Theodoris C.V., Liu L., Collins W.J., Blue K.W., Lee J.H., Meng X., Robbins R.C., Ivey K.N, & Srivastava D. (2015). NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothelium. Journal of molecular and cellular cardiology, 84, 13-23.
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3

Investigating miR-45-3p's Role in Atherosclerosis

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HAECs were obtained from ScienCell Research Laboratories, Inc. (cat. no. 6100) and cultured in Endothelial Cell Growth Medium (Sigma-Aldrich; Merck KGaA) supplemented with Endothelial Cell Growth Supplement (BD Biosciences), 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 100 U/ml penicillin-streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.). HAECs were cultured in a humidified atmosphere with 5% CO2 at 37˚C. To investigate the potential role of miR-45-3p in atherosclerotic progression, HAECs were exposed to ox-LDL at various concentrations (0, 25, 50 or 100 µg/l) for 24 h or to 50 µg/ml ox-LDL at various durations (0, 12, 24 or 48 h).
Liao L., Yang Q., Li H., Meng R, & Li Y. (2021). miR-454-3p prevents ox-LDL-induced apoptosis in HAECs by targeting TRPC3. Experimental and Therapeutic Medicine, 21(4), 323.
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4

Culturing Primary Aortic Endothelial Cells

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Primary human aortic endothelial cells (HAECs) were obtained from ScienCell™ Research Laboratories and were grown in complete Endothelial Cell Medium (ECM) supplemented with endothelial growth factors along with 5% FBS and 1% penicillin/streptomycin. All the cells were maintained at 37°C in a humidified atmosphere of 5% CO2.
Sonowal H., Pal P.B., Shukla K, & Ramana K.V. (2017). Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells. Oxidative Medicine and Cellular Longevity, 2017, 2769347.
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5

Primary Aortic Endothelial Cell Culture

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Primary human aortic endothelial cells (HAECs) were purchased from American Type Culture Collection (VA, USA). HAECs were cultured in endothelial cell media supplemented with 5% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 1% endothelial cell growth factor (ScienCell, CA, USA) and were grown in T-25 culture flasks at a starting density of 2.5 × 103 cells/cm2. The cells were maintained at 37°C in a 5% CO2 incubator. The media were changed the next day after thawing and subsequently every two days until the cells reached a confluency of about 70%. Then, the media were changed every day until the cells were 80–90% confluent. The cells were subcultured until the desired passage. Cells at passages 3 to 5 were used for assays.
Mohd Razali N.N., Teh S.S., Mah S.H., Yong Y.K., Ng C.T., Lim Y.M, & Fong L.Y. (2022). Protective Effects of Alternanthera sessilis Ethanolic Extract against TNF-α or H2O2-Induced Endothelial Activation in Human Aortic Endothelial Cells. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 8738435.
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6

Culturing Primary Human Aortic Endothelial Cells

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Primary human aortic endothelial cells (HAECs) were obtained from Science Cell (San Diego, USA) and stored at Beijing Yuhengfeng biotech, Inc. (Beijing, China). HAECs were cultured in accordance with the manufacturer's protocol and incubated in a humidifier at 37°C with 5% CO2. HAECs at passages 3–5 were collected for use in all experiments.
Chen Y., Li D., Xu Y., Zhang Y., Tao L., Li S., Jiang Y, & Shen X. (2014). Essential Oils from Fructus A. zerumbet Protect Human Aortic Endothelial Cells from Apoptosis Induced by Ox-LDL In Vitro. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 956824.
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7

Culturing Human Aortic Endothelial Cells

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HAECs was purchased from ScienCell (Carlsbad, CA, USA). The cells were cultured in endothelial cell medium supplemented with 10%FBS, 1% endothelial cell growth supplement (ECGS), 100 IU/ml penicillin, 0.1 mg/ml streptomycin (ScienCell) at 37 °C in a humidified atmosphere of 5% CO2. Confluent HAECs were maintained for 48 h with or without the presence of ox-LDL (50μg/mL; Beijing Solarbio Life Science Company) for further study.
Zhang S., Song G., Yuan J., Qiao S., Xu S., Si Z., Yang Y., Xu X, & Wang A. (2020). Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis. Journal of Biomedical Science, 27, 11.
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8

Regulating TRPC6 in Endothelial Cells

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HAECs were obtained from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured in Endothelial Cell Medium supplemented with endothelial cell growth factors, 5% FBS and 1% penicillin/streptomycin. The cells were maintained at 37°C with 5% CO2 and 95% air. HAECs were transiently transfected with miR-26a mimics, miR-26a inhibitors or negative controls (RiboBio Co., Ltd., Guangzhou, Guangdong, China), using Lipofectamine 2000 reagent (Invitrogen, CA, Carlsbad, USA) according to the manufacturer's instructions. TRPC6-expressing plasmid was transfected into HAECs. Briefly, cells were trypsinized and seeded for 24 h before transfection. The transfection mixture was dissolved in Opti-MEM serum-free medium and added to the cells. After 24 h of transfection, the medium was replaced by fresh medium with or without ox-LDL. After drug treatment, the cells were used for immunofluorescent staining or protein/RNA extraction.
Zhang Y., Qin W., Zhang L., Wu X., Du N., Hu Y., Li X., Shen N., Xiao D., Zhang H., Li Z., Zhang Y., Yang H., Gao F., Du Z., Xu C, & Yang B. (2015). MicroRNA-26a prevents endothelial cell apoptosis by directly targeting TRPC6 in the setting of atherosclerosis. Scientific Reports, 5, 9401.
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9

Silencing ELN and NOTCH3 in haSMCs

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haSMCs (Lonza) or haECs (ScienCell) were cultured up to passage 6 in M199 medium supplemented with 10% FBS, EGF, and FGF (PromoCell) or complete EC medium (ScienCell), respectively. For gene silencing, siRNA was transfected as described previously (68 (link)). Briefly, haSMCs were transfected with Lipofectamine 2000 (Life Technologies) containing siRNA targeted against ELN (Dharmacon, 50 nM) or NOTCH3 (Origene, 50 nM), or Scr RNA for 6 hours. Cells were then washed in M199 and cultured for 72 hours prior to collection for qRT-PCR or Western blot analysis.
Dave J.M., Chakraborty R., Ntokou A., Saito J., Saddouk F.Z., Feng Z., Misra A., Tellides G., Riemer R.K., Urban Z., Kinnear C., Ellis J., Mital S., Mecham R., Martin K.A, & Greif D.M. (2022). JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. The Journal of Clinical Investigation, 132(5), e142338.
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10

Culturing Human Aortic Endothelial Cells

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Human aortic endothelial cells (HAECs) were purchased from ScienCell Research Laboratories and cultured with the endothelial cell medium supplemented with fetal bovine serum (5%v/v), penicillin (10,000 U/mL), streptomycin (10,000 µg/mL), and endothelial cell growth supplement at 37 °C in a humidified incubator under 5% CO2 atmosphere. Only three to six passages of HAECs were used in these experiments.
Huang M., Wei R., Wang Y., Su T., Li P, & Chen X. (2018). The uremic toxin hippurate promotes endothelial dysfunction via the activation of Drp1-mediated mitochondrial fission. Redox Biology, 16, 303-313.
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