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Glass microfiber gf c filters

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Glass microfiber GF/C filters are laboratory filtration products designed for general-purpose filtration applications. They are made of high-quality glass microfibers and provide efficient retention of fine particulates. These filters are suitable for a variety of laboratory procedures that require reliable and consistent filtration performance.

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Lab products found in correlation

Whatman, by Cytiva (4 mentions) Ecolume liquid scintillation cocktail, by MP Biomedicals (3 mentions) Wallac 1450 microbeta counter, by PerkinElmer (1 mentions)

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GF/C filters (141 citations) Glass microfiber filters (64 citations) Glass microfiber (9 citations)

4 protocols using glass microfiber gf c filters

1

Preparation of Algae-Derived Media

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The CM, Exp-CM and Stat-CM, were obtained from exponential and stationary E. huxleyi CCMP379 mono-cultures (Table 1), respectively, by gentle gravity filtration on Whatman glass microfiber GF/C filters (pore size of 1.2 µm). This method was chosen to prevent lysis of algal cells during the procedure and thus ensuring that only extracellular algae-derived metabolites, infochemicals, and other components will reside in the media. CM were subsequently filtered through 0.22 µm using Stericup vacuum filters. Exp-CM and Stat-CM were harvested on the same day of the experiment. When indicated, 100 µM DMSP was added to Exp-CM, herein Exp-CM +DMSP. This concentration mimics that present in the phycosphere of E. huxleyi in stationary phase (Barak-Gavish et al., 2018 (link); Seymour et al., 2017 (link)). MM was based on artificial sea water (ASW) (Goyet and Poisson, 1989 (link)) supplemented with basal medium (-Tris) (containing essential nutrients) (Baumann and Baumann, 1981 ), vitamin mix (González et al., 1997 (link)), 0.5 mM NaNO3, and metal mix of k/2 medium (Keller et al., 1988 (link)). For the transcriptome experiment, MM were supplemented with 1 gr L–1 glycerol. When indicated, 100 µM DMSP was added to MM, herein MM +DMSP.
Barak-Gavish N., Dassa B., Kuhlisch C., Nussbaum I., Brandis A., Rosenberg G., Avraham R, & Vardi A. (2023). Bacterial lifestyle switch in response to algal metabolites. eLife, 12, e84400.
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2

GTPγS Binding Assay for GPCR Activity

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CHO-hDOPr cell membranes (as prepared above, 10 μg/well) were incubated for 1 h at 30 °C in buffer comprising 50 mM Tris-HCl (pH 7.4), 1 mM EDTA, 5 mM MgCl2, 100 mM NaCl, 0.1 nM [35S]GTPγS, and 30 μM GDP (guanosine 5-diphosphate) in a final volume of 200 μL. Orthosteric and allosteric ligands were also included, with SNC80 used as the maximal standard and assay buffer used to assess basal [35S]GTPγS binding. The reaction was terminated by filtration through glass microfiber GF/C filters (Whatman) using a Brandell harvester. The filters were rinsed, dried, and radioactivity was counted by liquid scintillation counting using EcoLume liquid scintillation cocktail (MP Biomedicals) in a Wallac 1450 McroBeta counter (PerkinElmer).
Burford N.T., Livingston K.E., Canals M., Ryan M.R., Budenholzer L.M., Han Y., Shang Y., Herbst J.J., O'Connell J., Banks M., Zhang L., Filizola M., Bassoni D.L., Wehrman T.S., Christopoulos A., Traynor J.R., Gerritz S.W, & Alt A. (2015). Discovery, Synthesis, and Molecular Pharmacology of Selective Positive Allosteric Modulators of the δ-Opioid Receptor. Journal of medicinal chemistry, 58(10), 4220-4229.
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3

Displacement of Opioid Receptor Ligand

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Displacement of 3H-diprenorphine (DPN) (Kd = 0.15 nM) by increasing concentrations of orthosteric ligand was measured in the presence of vehicle (1% DMSO) or allosteric ligand. Briefly, C6-μ cell membranes (10 μg/well, prepared as described above) were incubated in a buffer (50 mM Trizma base pH 7.4, 1 mM EDTA, 5 mM MgCl2, 100 mM NaCl) containing orthosteric ligand, allosteric ligand (or vehicle), 10 μM GTPγS, and 3H-DPN (0.2–0.3 nM) for 60–90 min shaking at room temperature. Nonspecific binding was assessed in the presence of 10 μM naloxone. Reactions were terminated by rapid filtration through glass microfiber GF/C filters (Whatman) using a Brandell harvester and washed three times using ice-cold 50 mM Trizma buffer pH 7.4. Filters were dried and radioactivity was measured using liquid scintillation counting with EcoLume liquid scintillation cocktail (MP Biomedicals) in a Wallac 1450 MicroBeta counter.
Bisignano P., Burford N.T., Shang Y., Marlow B., Livingston K.E., Fenton A.M., Rockwell K., Budenholzer L., Traynor J.R., Gerritz S.W., Alt A, & Filizola M. (2015). Ligand-Based Discovery of a New Scaffold for Allosteric Modulation of the μ-Opioid Receptor. Journal of chemical information and modeling, 55(9), 1836-1843.
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4

Cell Membrane Binding Assay

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Cell membranes (as prepared above, 10 μg/well) were incubated in the following mixture for 90 min at 25 °C: assay buffer (50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM EDTA, 5 mM MgCl2, 10 μM GTPγS), various concentrations of orthosteric and allosteric ligand, and 0.35-0.45 nM [3H]DPN. Nonspecific binding was determined in the presence of 10 μM naloxone. Reactions were terminated by rapid filtration through glass microfiber GF/C filters (Whatman) using a Brandell harvester and washed three times using cold 50 mM Tris-HCl buffer. Filters were dried in a 50 °C oven, and radioactivity was measured by liquid scintillation counting with EcoLume liquid scintillation cocktail (MP Biomedicals) in a Wallac 1450 MicroBeta counter (PerkinElmer).
Burford N.T., Livingston K.E., Canals M., Ryan M.R., Budenholzer L.M., Han Y., Shang Y., Herbst J.J., O'Connell J., Banks M., Zhang L., Filizola M., Bassoni D.L., Wehrman T.S., Christopoulos A., Traynor J.R., Gerritz S.W, & Alt A. (2015). Discovery, Synthesis, and Molecular Pharmacology of Selective Positive Allosteric Modulators of the δ-Opioid Receptor. Journal of medicinal chemistry, 58(10), 4220-4229.
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