We prepared 100 μL hydrogels for each condition (n = 4) and placed them in 1.5 mL centrifuge tubes (sterilized by autoclave). To each tube, we added 500 μL sterile phosphate buffered saline (PBS, pH = 7.4). We placed the gels in an incubator set to 37 °C. At specified timepoints (1.5h, 3h, 6h, 12h, 24h. then daily for 2 weeks), we removed the entire volume of PBS and replaced it with fresh PBS.27 (link) We stored the collected release samples at −80 °C for the duration of the study. To determine the amount of released SDF-1α at each timepoint, we performed an enzyme-linked immunosorbent assay (ELISA, Peprotech) according to the manufacturer’s instructions. We calculated the cumulative amount of SDF-1α released at each timepoint, and propagated the error through each successive calculation.
Sdf 1α
SDF-1α is a recombinant protein produced in E. coli. It is a chemokine that belongs to the CXC chemokine family. SDF-1α is involved in the regulation of various cellular processes.
Lab products found in correlation
85 protocols using sdf 1α
Heparin-Modulated SDF-1α Release from Hydrogels
SDF1α Stimulation Kinetics
SDF1α Stimulation Kinetics
Hydrogel Crosslinking with BMP-7 and SDF-1α
SDF-1α Loading on OPF/BP Hydrogels
Transwell Assay for Fused RPMI8226 Cells
We performed a transwell migration assay (Costar, Cambridge, MA, USA) using the fusion cell in the presence of BM-MSCs, which were cultured in the lower chambers. Three groups were included in the assay: (1) control, upper chamber: fused RPMI8226 cells, and lower chamber: DMEM-LG supplemented with 0.5% FBS; (2) MM cell, upper chamber: RPMI8226 cells, and lower chamber: DMEM-LG supplemented with 0.5% FBS and 50 ng/ml SDF-1α (Peprotech, Rocky Hill, NJ, USA); (3) fusion cell, upper chamber: fused RPMI8226, and lower chamber: DMEM-LG supplemented with 0.5% FBS and 50 ng/ml SDF-1α. In brief, the fused cells and RPMI8226 cells were suspended in 0.5% FBS medium, and 5 × 105 cells were placed in the upper chambers of the transwell plates with or without SDF-1
Fabrication of Biodegradable Vascular Grafts
Dual-layer Vascular Graft Fabrication
Hydrogel-Based Cell Migration Promotion
Trans-well migration of megakaryocytes
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