The following antibodies were used: anti-FLAG (Sigma, F7425), anti-IKZF3 (Cell Signaling, #15103), anti-IKZF1 (Cell Signaling, #9034), anti-RUNX1 (Abcam, ab92336), anti-RUNX3 (Cell Signaling, #9647), anti-CBFβ (Santa Cruz, sc-20693), anti-CRBN (Sigma, HPA045910), anti-ubiquitin (K48) (EMD Millipore, #05–1307), anti-TUBULIN (Santa Cruz, sc-8035), anti-VINCULIN (Santa Cruz, sc-73614), anti-Rabbit IgG-HRP (GE Healthcare, NA934V), and anti-mouse IgG-HRP (GE Healthcare, NA931V). The following agarose beads were used: anti-FLAG M2 Affinity Gel (Sigma, A2220) and Glutathione Sepharose 4B (GE Healthcare, #17075601). The following in vitro translation kit was used: TNT T7 Coupled Reticulocyte Lysate Systems (Promega, L4610). Benzonase was used according to manufacturer (Sigma, E1014). The following compounds were used: Lenalidomide (Sigma, CDS022536), Pomalidomide (Sigma, P0018), Bortezomib (Millennium Pharmaceuticals), and AI-10–104 was kindly provided by Dr. John H. Bushweller.
Anti ikzf3
Manufactured by Cell Signaling Technology
Anti-IKZF3 is a laboratory reagent used to detect and study the IKZF3 protein. It is a specific antibody that binds to the IKZF3 protein, allowing researchers to identify and quantify its presence in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Lenalidomide, by Merck Group (2 mentions)
Anti ikzf1, by Cell Signaling Technology (2 mentions)
Anti runx1, by Abcam (2 mentions)
Anti cbfβ, by Santa Cruz Biotechnology (2 mentions)
Anti crbn, by Merck Group (2 mentions)
Anti ubiquitin k48, by Merck Group (2 mentions)
Anti vinculin, by Santa Cruz Biotechnology (2 mentions)
Anti rabbit igg hrp, by GE Healthcare (2 mentions)
Anti mouse igg hrp, by GE Healthcare (2 mentions)
Tnt t7 coupled reticulocyte lysate system, by Promega (2 mentions)
Pomalidomide, by Merck Group (2 mentions)
Anti tubulin, by Santa Cruz Biotechnology (2 mentions)
Anti runx3, by Cell Signaling Technology (2 mentions)
Anti flag, by Merck Group (2 mentions)
Anti flag m2 affinity gel, by Merck Group (2 mentions)
Glutathione sepharose 4b, by GE Healthcare (2 mentions)
Benzonase, by Merck Group (2 mentions)
2 protocols using anti ikzf3
1
Characterizing CRBN-mediated Protein Degradation
2
Immunoprecipitation Assay for E3 Ligase Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were used: anti-FLAG (Sigma, F7425), anti-IKZF3 (Cell Signaling, #15103), anti-IKZF1 (Cell Signaling, #9034), anti-RUNX1 (Abcam, ab92336), anti-RUNX3 (Cell Signaling, #9647), anti-CBFβ (Santa Cruz, sc-20693), anti-CRBN (Sigma, HPA045910), anti-ubiquitin (K48) (EMD Millipore, #05-1307), anti-TUBULIN (Santa Cruz, sc-8035), anti-VINCULIN (Santa Cruz, sc-73614), anti-Rabbit IgG-HRP (GE Healthcare, NA934V), and anti-mouse IgG-HRP (GE Healthcare, NA931V). The following agarose beads were used: anti-FLAG M2 Affinity Gel (Sigma, A2220) and Glutathione Sepharose 4B (GE Healthcare, #17075601). The following in vitro translation kit was used: TNT T7 Coupled Reticulocyte Lysate Systems (Promega, L4610). Benzonase was used according to manufacturer (Sigma, E1014). The following compounds were used: Lenalidomide (Sigma, CDS022536), Pomalidomide (Sigma, P0018), Bortezomib (Millennium Pharmaceuticals), and AI-10-104 was kindly provided by Dr. John H. Bushweller.
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