All fresh tissues were fixed for 30 minutes at room temperature in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA), followed by gradient ethanol dehydration, paraffin embedding, sectioning (with a thickness of 6 μm), and deparaffinization in xylene. The tissue sections were stained with haematoxylin-eosin (H & E, Sigma-Aldrich, St. Louis, MO, USA), clarified in xylene (Sigma-Aldrich, St. Louis, USA), and mounted in neutral resin (Sigma-Aldrich, St. Louis, MO, USA).
Haematoxylin eosin h e
Manufactured by Merck Group
Sourced in United States
Haematoxylin-eosin (H&E) is a common staining technique used in histology and pathology to visually differentiate different cellular and tissue structures. The stain is composed of two dyes, haematoxylin and eosin, which selectively stain different components of cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Xylene, by Merck Group (2 mentions)
Neutral resin, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Analysis ls software, by Olympus (1 mentions)
Cx21 light microscope, by Olympus (1 mentions)
Ethyl alcohol, by Merck Group (1 mentions)
Bx51 light microscope, by Olympus (1 mentions)
3 protocols using haematoxylin eosin h e
1
Tissue Fixation and Histological Staining
2
Histological Analysis of Myocardial Tissue
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The myocardial tissue block was fixed in 4% paraformaldehyde and embedded in paraffin, then cut to a thickness of 4 μm. Sections were dehydrated with different concentrations of alcohol. The sections were stained with haematoxylin–eosin (HE) (Sigma‐Aldrich) and observed under a microscope (Olympus).
3
Histopathological Examination of Tadpoles
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At the end of the experiment (hour 120), all surviving tadpoles were evaluated for histopathological changes by fixing them in Bouin's solution for 8 h and then preserving them in 70 % ethyl alcohol (Sigma-Aldrich) until the preparation day. On the preparation day, the tadpoles were passed through 80 %, 90 %, 96 %, and absolute ethyl alcohol series and later through xylene (Sigma-Aldrich) and paraffin series to be embedded in paraffin blocks. We made 8-µmthick cross sections and stained them with haematoxylin & eosin (H&E; Sigma-Aldrich) for histopathological examination with an Olympus CX21 light microscope. We also took photographs with a camera adapted to the Olympus BX51 light microscope and analysed them with Olympus Analysis LS software.
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