The following antibodies were used: α-Tubulin (T9026); Acetyl-Histone H4 (06–866 lot#2554112, EMD Millipore (Billerica, MA)); CHD4 (A301-081A, Bethyl Laboratories (Montgomery, TX)); FITC anti-mouse (715-095-150 lot#115855, Jackson ImmunoResearch (West Grove, PA)); FLAG M2 (F1804 lot#SLBG5673V and lot#124K6106); FLAG M2 (F3165 lot#SLBL1237V); HDAC2 (ab7029, lot#GR88809-7, Abcam (Cambridge, UK)); HRP anti-mouse (115-035-146, Jackson ImmunoResearch); MBD2 (A301-632A, Bethyl); mouse IgG (315-005-003 lot#120058, Jackson ImmunoResearch); MTA2 (ab8106 lot#GR185489-3, Abcam); TRITC anti-rabbit (711-025-152 lot#114768, Jackson ImmunoResearch); rabbit monoclonal antibodies against DUX4 (E5-5 and E14-3) were produced in collaboration with Epitomics and are described elsewhere (Geng et al., 2012 (link)).
Chd4 a301 081a
Manufactured by Fortis Life Sciences
Sourced in United Kingdom
The CHD4 (A301-081A) is a lab equipment product offered by Fortis Life Sciences. It is designed to facilitate specific research applications. The core function of this product is to provide researchers with a tool to study the relevant biological processes. No further details can be provided while maintaining an unbiased and factual approach.
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Lab products found in correlation
Hrp anti mouse, by Jackson ImmunoResearch (1 mentions)
Flag m2, by Abcam (1 mentions)
Ab1765, by Abcam (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Transfer buffer, by Thermo Fisher Scientific (1 mentions)
Hdac2 sc 7899, by Santa Cruz Biotechnology (1 mentions)
Rbbp4, by Abcam (1 mentions)
Sall4, by Abcam (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
2 protocols using chd4 a301 081a
1
Antibody Validation for Protein Analysis
2
Protein Complex Analysis via Gel Electrophoresis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For denaturing PAGE, samples were separated in NuPAGE Bis-Tris 4–12% gels (Life Technologies) using MOPS running buffer according to manufacturer's instructions. Gels were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using XCell Transfer Kit and Transfer Buffer (Life Technologies) according to manufacturer's instructions. BN-PAGE gels were first incubated in 8% acetic acid prior to transfer to PVDF membranes (Life Technologies). Protein detection was performed using the following antibodies: FLAG (M2-peroxidase labeled, A8592, Sigma-Aldrich), Sall4 (ab29112, Abcam, Cambridge, UK), Chd4 (A301–081A, Bethyl Laboratories Inc., Montgomery, TX), Hdac2 (sc-7899, Santa Cruz, Biotechnology Inc., Dallas, TX), Wdr5 (A302–430A, Bethyl Laboratories Inc.), Wdr5 (A302–429A, Bethyl Laboratories Inc.), and Rbbp4 (ab1765, Abcam).
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