Sprague dawley strain

Manufactured by Charles River Laboratories

Sourced in Japan, Italy, France

Sprague-Dawley strain is a widely used laboratory rat model. It is a outbred albino rat strain developed at the Sprague-Dawley Animal Company. The Sprague-Dawley strain is commonly used in a variety of research applications.

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15 protocols using sprague dawley strain

Dietary Effects on Sprague-Dawley Rats

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Male rats of the Sprague-Dawley strain were obtained from Charles River (Calco, Como, Italy). All rats were housed individually at a temperature of 23 °C ± 1 °C with a 12-h light/dark cycle (06.30–18.30). All experimental procedures involving animals met the guidelines set by the Italian Health Ministry and were approved by “Comitato Etico-Scientifico per la Sperimentazione Animale” of the University of Naples “Federico II” (260/2015-PR).
Middle-aged rats (11 months old) were divided in three groups, each composed of eight rats. One group of rats was euthanized at the beginning of the experiment for determination of initial values of body composition, while the other two groups were fed a high fat-high fructose (HFF) or low-fat diet (LF) for 4 weeks. The composition of the two diets is reported in Table 1. During the dietary treatment, the rats were housed one per cage, and body weight, food and water intake were monitored daily and feces were collected. At the end of the experimental period, the animals were anaesthetized with zolazepam plus tiletamine (1:1) (30 mg kg⁻¹ i.p.) and euthanized by decapitation, blood and liver were harvested, and the carcasses used for determination of body composition.

Mazzoli A., Crescenzo R., Cigliano L., Spagnuolo M.S., Cancelliere R., Gatto C, & Iossa S. (2019). Early Hepatic Oxidative Stress and Mitochondrial Changes Following Western Diet in Middle Aged Rats. Nutrients, 11(11), 2670.

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Sprague-Dawley Rat Newborn Model

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Male newborn rats were obtained in our laboratory by mating females with males of the same colony of specific pathogen-free rats of the Sprague-Dawley strain purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). One foster mother with 8 male newborns were housed together in plastic Econ cages (W 340 mm × D 450 mm × H 185 mm) with bedding (White flakes: Charles River Laboratories Japan, Inc.) in an environmentally controlled animal room (temperature, 23 ± 3ºC; relative humidity, 50 ± 20%; air ventilation rate, 10–15 times per hour; lighting, 12 h/12 h light/dark cycle) and fed an irradiation-sterilized pelleted diet (NMF, Oriental Yeast Co., Ltd., Tokyo, Japan) and tap water ad libitum. Finally, a total of fifty 6-day-old male rats were subjected to the experiment. The protocol of this study was reviewed and approved by the Animal Care and Use Committee of BoZo Research Center.

Ohira T., Saito T., Ando R., Tamura K, & Hoshiya T. (2014). Systemic Histopathology of Infant Rats Exposed to Busulfan. Journal of Toxicologic Pathology, 27(1), 25-29.

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Neuronal Culture and Transduction Protocol

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Pregnant rats (E17) of the Sprague-Dawley strain were purchased from Charles River. Embryos were surgically removed from the mothers and cortices dissected in Hanks Balanced salt solution (Gibco). The meninges were removed and cells dissociated using a papain dissociation system (Worthington) before being resuspended in Neurobasal medium A supplemented with antibiotic-antimycotic solution (Gibco), L-glutamine substitute (GlutaMAX™; Gibco) and factor B27 (Gibco). Cells were plated on poly-D-lysine coated glass coverslips at a density of 5 × 10⁵ cells/well and incubated at 37 °C in 5% CO₂ with half media changes every 3 days. Cells were transduced with AAV-A53T, AAV-Empty and AAV-Ub^G76V-GFP 2 days post-isolation at a multiplicity of infection (MOI) of 3000. Media containing AAV vectors were removed after 72 h and cells were fixed with 4% PFA for immunofluorescence staining at 8 days post-isolation.

McKinnon C., De Snoo M.L., Gondard E., Neudorfer C., Chau H., Ngana S.G., O’Hara D.M., Brotchie J.M., Koprich J.B., Lozano A.M., Kalia L.V, & Kalia S.K. (2020). Early-onset impairment of the ubiquitin-proteasome system in dopaminergic neurons caused by α-synuclein. Acta Neuropathologica Communications, 8, 17.

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Postnatal Hippocampal Protein Distribution

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For protein distribution measurements we dissected hippocampi from postnatal day 0 to 1 rat pups of either sex (Sprague-Dawley strain; Charles River Laboratories). All experiments complied with national animal care guidelines and the guidelines issued by the Max Planck Society, and were approved by local authorities.

Sartori F., Hafner A.S., Karimi A., Nold A., Fonkeu Y., Schuman E.M, & Tchumatchenko T. (2020). Statistical Laws of Protein Motion in Neuronal Dendritic Trees. Cell Reports, 33(7), 108391.

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Transgenic Astrocyte and Endothelial Cell Isolation

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A colony of transgenic 8.3 tdTomato /BAC eGFP GLT-1 (“dual reporter”) mice, which are homozygous for both traits, were maintained at the animal facility of the Children’s Hospital of Philadelphia (Regan et al. 2007 (link); Yang et al. 2011b (link)). For studies involving astrocytes or endothelial cells prepared from rats, the Sprague-Dawley strain (RRID:RGD_734476, Charles River Laboratories, Wilmington, MA) was used as described below. All studies were approved by the Institutional Animal Care and Use Committee of the Children’s Hospital of Philadelphia and followed the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. Animals were housed in standard controlled temperature, humidity, and light, and had ad libitum access to food and water.

Lee M.L., Martinez-Lozada Z., Krizman E.N, & Robinson M.B. (2017). Brain endothelial cells induce astrocytic expression of the glutamate transporter GLT-1 by a Notch-dependent mechanism. Journal of neurochemistry, 143(5), 489-506.

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Muscle Lim Protein Deficient Mice in Research

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Animal experiments were performed in accordance with European ethical regulation (Directive 2010/63/EU) and approved by the local animal ethics committee of Linköping (permit numbers S43-15, 1369, and 2713) and Göteborg (permit 1852-2018) in Sweden, and the responsible government agency of Unterfranken (RUF-55.2.2-2532-2-659) in Germany. Animals were housed on a 12 h light/12 h dark cycle with free access to chow and water, in 45–65% humidity and ambient temperature at 20–24 °C.
The background of the muscle lim protein-deficient mice (Mlp^−/−), also known as cysteine and glycine-rich protein 3 (Csrp3^−/−), is a hybrid cross of original 129/Sv background^{29 (link)} and C57BL/6 N strain. The mice were bred in-house for multiple generations. Heterozygous males and females were bred to obtain both WT and Mlp^−/− animals. Both male and female mice were utilized for experiments at the age of 10-14 weeks. If animals of other ages were used, a description was mentioned in the figure legend or in the Source Data file.
Rats from Sprague Dawley strain were purchased from Charles River Laboratories and bred in-house to obtain neonatal pups, which were dissected at the age of 3 days to harvest their hearts.

ElBeck Z., Hossain M.B., Siga H., Oskolkov N., Karlsson F., Lindgren J., Walentinsson A., Koppenhöfer D., Jarvis R., Bürli R., Jamier T., Franssen E., Firth M., Degasperi A., Bendtsen C., Menzies R.I., Streckfuss-Bömeke K., Kohlhaas M., Nickel A.G., Lund L.H., Maack C., Végvári Á, & Betsholtz C. (2024). Epigenetic modulators link mitochondrial redox homeostasis to cardiac function in a sex-dependent manner. Nature Communications, 15, 2358.

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IG Conditioning Using Sprague Dawley Rats

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Adult female rats (n=20, Sprague Dawley strain, Charles River) weighing 295–345 g were used as in our prior IG conditioning studies using SOA solutions (Pérez et al., 1998 (link); Myers & Sclafani, 2003 (link); Yiin et al., 2005 (link)). The rats were individually housed in standard wire-mesh cages in a test room maintained at 21°C under a 12:12 h light:dark cycle (lights on at 0800 hours). Experimental protocols were approved by the Institutional Animal Care and Use Committee at Brooklyn College and were performed in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals.

Sclafani A, & Ackroff K. (2020). Nutrient-conditioned intake stimulation does not require a distinctive flavor cue in rats. Appetite, 154, 104793.

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Rat Hippocampal Neuron Culture Protocol

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Dissociated rat hippocampal neuron cultures were prepared and maintained essentially as described previously (Aakalu et al., 2001 (link); Banker and Goslin, 1990 ). Briefly, we dissected hippocampi from postnatal day 0 to 1 rat pups of either sex (Sprague-Dawley strain; Charles River Laboratories), dissociated them with papain (Sigma) and plated them at a density of 40–30

\times

10³ cells/cm² onto poly(d-lysine)-coated (BD Biosciences) glass-bottom Petri dishes (MatTek). Hippocampal neurons were maintained, fed weekly with a neuronal growth medium and allowed to mature in a humidified atmosphere at 37° C and 5% CO₂ in a growth medium (Neurobasal-A supplemented with B27 and GlutaMAX-I, Life Technologies) for 8 days in vitro.

Sartori F., Hafner A.S., Karimi A., Nold A., Fonkeu Y., Schuman E.M, & Tchumatchenko T. (2020). Statistical Laws of Protein Motion in Neuronal Dendritic Trees. Cell Reports, 33(7), 108391.

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Preparation of Cortical Neuron Cultures

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Mouse cortical neurons were prepared from newborn pups (Consortium for Functional Glycomics). Rat cortical neurons were prepared from E18–19 embryos dissected from pregnant rats (Sprague Dawley strain, purchased from Charles River). Dissected cortices were dissociated with papain according to the manufacturer’s instructions (Worthington Biochemical). Cells were plated on poly-D-lysine-coated coverslips in 24-well plates. Experiments were carried out using days in vitro (DIV) 11–13 for mouse neurons and DIV 13–15 for rat neurons.

Yin L., Masuyer G., Zhang S., Zhang J., Miyashita S.I., Burgin D., Lovelock L., Coker S.F., Fu T.M., Stenmark P, & Dong M. (2020). Characterization of a membrane binding loop leads to engineering botulinum neurotoxin B with improved therapeutic efficacy. PLoS Biology, 18(3), e3000618.

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Sprague-Dawley Rat Husbandry for Experiments

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Standard housed (light–dark cycle (12/12); temperature: 21 ± 1 °C; humidity: 50–60%; animals per cage: 3–4) male and female rats of the Sprague–Dawley strain (Charles River, Italy), with body weights approximately 220–250 g, supplied with food and water ad libitum, were used for the experimental design. The treatment of animals was carried out according to the Declaration of Helsinki Guiding Principles on Care and Use of Animals (DHEW Publication, NHI 80-23) and the European Communities Council Directives of 24 November 1986 (86/609/EEC). The project was approved by the Bulgarian Food Safety Agency.

Ivanova N., Atanasova M., Nenchovska Z, & Tchekalarova J. (2023). Sex-Dependent Effect of Chronic Piromelatine Treatment on Prenatal Stress-Induced Memory Deficits in Rats. International Journal of Molecular Sciences, 24(2), 1271.

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