The interaction between α-LA and β-CN was observed by SEC [33 (link)]. Samples (100 µM) following aggregation were centrifuged at 14 000×g for 30 min at 4°C and subsequently loaded (500 µl) onto a Superdex 200 10/300 GL analytical-SEC (GE Healthcare, Illinois, U.S.A.) which was equilibrated with 100 mM ammonium acetate (pH 7.0) at a flow rate of 0.4 ml/min at room temperature. The SEC column was calibrated using standards (Sigma–Aldrich, Missouri, U.S.A.) containing bovine thyroglobulin (670 kDa), bovine γ-globulin (158 kDa), chicken ovalbumin (44 kDa) and horse myoglobin (17 kDa).
Superdex 200 10 300 gl analytical
Manufactured by GE Healthcare
Sourced in United States
Superdex 200 10/300 GL is a size exclusion chromatography column used for the separation and analysis of proteins, peptides, and other macromolecules. It is designed for analytical-scale applications and provides high-resolution separation of molecules with a molecular weight range of 10,000 to 600,000 Daltons.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using superdex 200 10 300 gl analytical
1
SEC Analysis of α-LA and β-CN Interaction
2
Size-Exclusion Chromatography Protein Analysis
3
Characterizing HIV-1 Env Proteins Using FPLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, a Superdex-200 10/300 GL analytical gel filtration column (GE healthcare) equilibrated in PBS (pH 7.4) buffer was utilized for characterizing BG505SOSIP, BG505SOSIP D1, D2, D3 and NFL Wt, D1, D2, and D3 using a SHIMADZU liquid chromatography FPLC system connected in-line with UV (SHIMADZU), refractive index (WATERS corp.) and MALS detectors (mini DAWN TREOS, Wyatt Technology corp.) for molecular weight characterization. UV, MALS and RI data were collected and analysed using ASTRA™ software (Wyatt Technology).
4
Size-Exclusion Chromatography Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Size-exclusion chromatography (SEC) was performed at 4°C on an AKTA FPLC (GE Healthcare). Approximately 6 mg of soluble protein was applied to a Superdex 200 10/300 GL analytical gel filtration column (GE Healthcare) equilibrated in PBS, pH 7.2 at a flow rate of 0.5 mL min−1. Fractions were collected every 0.5 mL. The elution volumes of molecular weight standards (Thyroglobulin, 670,000 Da; γ-globulin, 158,000 Da; Ovalbumin, 44,000 Da; Myoglobin, 17,000 Da; Vitamin B12, 1,350 Da; Biorad) were additionally measured to calibrate the column (Figure 2A ). Fraction 4 (concentration ~1 mg/mL) was deemed most likely to contain a high number of large complexes, as determined by A280, and was subjected to further proteomic and structural analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!