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Humidified cell incubator

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The Humidified Cell Incubator is a laboratory equipment designed to provide a controlled environment for cell culture. Its core function is to maintain a stable temperature and humidity level within the incubator chamber to support the optimal growth and proliferation of cells.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Cell counter, by Countstar (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Human np cells, by ScienCell (1 mentions) Tnf α, by Novoprotein (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Sw480, by BNCC (1 mentions) Ncm460, by BNCC (1 mentions) Ges 1, by National Collection of Authenticated Cell Cultures (1 mentions) 125 ml shake flasks, by Corning (1 mentions) Cho k1, by Thermo Fisher Scientific (1 mentions) Mycoblue mycoplasma detector, by Vazyme (1 mentions) Wild type hek293 cells hek293t, by National Collection of Authenticated Cell Cultures (1 mentions)

9 protocols using humidified cell incubator

1

Rat and Human Nucleus Pulposus Cell Cultures

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The experiments were approved by the Ethics Committee of Sun Yat-sen Memorial Hospital, Sun Yat-sen University (No. [2020]-405), and the Animal Care and Use Committee of Sun Yat-sen University (No. SYSU-IACUC-2020-B0933). Rat NP tissue was collected, cut into pieces and digested into cells which were then cultured in DMEM (HyClone, Logan, USA) with 10% FBS (Gibco, New York, USA), penicillin G in a humidified cell incubator (Thermo Fisher Scientific, Waltham, USA). Human NP cells were obtained from ScienCell (ScienCell, San Diego, USA) with authentication. NF-κB activator 1 (MedChemExpress, Princeton, USA) was added after TNF-α (Novoprotein, Suzhou, China) stimulation of rat NP cells.
Zhang Y., Li S., Hong J., Yan J., Huang Z., Wu J., Deng Z., Qin T., Xu K, & Ye W. (2022). Chromobox homolog 4 overexpression inhibits TNF-α-induced matrix catabolism and senescence by suppressing activation of NF-κB signaling pathway in nucleus pulposus cells: CBX4 overexpression inhibits TNF-α-induced matrix catabolism and senescence. Acta Biochimica et Biophysica Sinica, 54(7), 1021-1029.
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2

Cell Line Culturing Protocols

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AGS cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, PR China). MKN-45 cells were obtained from the National Cell Line Resource Infrastructure (Beijing, PR China). AGS cells were cultured in F12 medium (HyClone, Logan, UT, USA) supplemented with 12% FBS (Gibco, Carlsbad, CA, USA). MKN-45 cells were cultured in RPMI-1640 (Gibco) containing 10% FBS. All cells were cultured in a humidified cell incubator (Thermo Fisher Scientific, Waltham, MA, USA) at 37°C, 95% air and 5% CO2, verified by short tandem repeat analysis and tested for absence of mycoplasma.
Liu F., Wang Y., Yang Z., Cui X., Zheng L., Fu Y., Shao W., Zhang L., Yang Q, & Jia J. (2022). KDM6B promotes gastric carcinogenesis and metastasis via upregulation of CXCR4 expression. Cell Death & Disease, 13(12), 1068.
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3

Culturing Mouse Embryonic Fibroblasts

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Mouse embryonic fibroblast NIH/3T3 cells, purchased from the National Infrastructure of Cell Line Resource, were cultured in complete medium, composed of Dulbecco’s Modified Eagle Medium (Gibco, Grand Island, NY, USA), 10% fetal bovine serum (Gibco), 100 U/mL penicillin, and 100 μg/mL Streptomycin, at 37 °C with 5% CO2 in the humidified cell incubator (Thermo Scientific, Waltham, MA, USA) and passaged every 3–4 days.
Li D.Y., Song J.D., Liang Z.Y., Oskouei K., Xiao X.Q., Hou W.Z., Li J.T., Yang Y.S., Wang M.L, & Murbach M. (2020). Apoptotic Effect of 1800 MHz Electromagnetic Radiation on NIH/3T3 Cells. International Journal of Environmental Research and Public Health, 17(3), 819.
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4

Cultivation of Human Colon Cell Lines

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The the human colon cancer cell lines LoVo (BNCC338601) and SW480 (BNCC288146) and human normal intestinal epithelial cell line NCM460 (BNCC353657) were obtained from the BeNa Culture Collection (www.bnbio.com, Beijing, China). These cells were derived from ATCC (Manassas, VA, USA) and have been authenticated using short tandem repeat (STR) markers. In addition, the cells have not been tested for mycoplasma contamination. Cells were cultured in RPMI-1640 (Gibco, Rockville, MD, USA) containing 10% foetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA). The cells were maintained in a humidified cell incubator (Thermo Fisher Scientific, Waltham, USA) atmosphere of 5% CO2 at 37 °C.
Cong J., Gong J., Yang C., Xia Z, & Zhang H. (2021). MiR-200c/FUT4 axis prevents the proliferation of colon cancer cells by downregulating the Wnt/β-catenin pathway. BMC Cancer, 21, 2.
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5

Culturing Gastric Cancer Cell Lines

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Human gastric cancer cell lines SGC7901 and BGC823 were purchased from Cell-Land Biotech Co., Ltd. (Hangzhou, China; www.cell-land.com). Non-malignant gastric epithelium cell line GES1 was obtained from Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). All cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and maintained at 37°C in an atmosphere containing 5% CO2 with saturated humidity in a humidified cell incubator (Thermo Fisher Scientific, Inc.). The cells were collected during their logarithmic phase and stored at −80°C for further study.
Yang Z., Xie Q., Chen Z., Ni H., Xia L., Zhao Q., Chen Z, & Chen P. (2018). Resveratrol suppresses the invasion and migration of human gastric cancer cells via inhibition of MALAT1-mediated epithelial-to-mesenchymal transition. Experimental and Therapeutic Medicine, 17(3), 1569-1578.
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6

Culturing Osteosarcoma Cell Lines

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Cell culture. The OS cell lines MG63 and U2OS and the human osteoblast hFOB1.19 cell line were obtained from the Shanghai Institute of Life Sciences, Chinese Academy of Sciences. OS cells were cultured in high glucose Dulbecco's modified Eagle medium (DMEM; HyClone; pH 7.0-7.4) with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 IU/ml penicillin, and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.). All cells were incubated in a humidified cell incubator (Thermo Fisher Scientific, Inc.) at 37˚C with 5% CO 2 . The adherent OS cells were trypsinized, collected by centrifugation, resuspended in fresh medium and passaged at a ratio of 1:4.
Xu S., Wang Y., Que Y., Ma C., Cai S., Wang H., Yang X., Yang C., Cheng C., Zhao G, & Hu Y. (2020). Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway. Oncology reports, 43(5).
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7

Cultivation and Infection of Cell Lines

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The human HSC-3 and mouse SCC-7 cell lines were kindly provided by Prof. Qianming Chen (State Key Laboratory of Oral Diseases, Sichuan University). Both cell lines were cultured in DMEM (HyClone, USA) with 10% FBS (Gibco, Australia) at 37°C in a humidified cell incubator (Thermo Fisher Scientific, USA) with 5% CO2. Cells at 75% confluence were used for these studies. According a previous study [23 (link)], a MOI of 200 was selected for use in subsequent experiments to detect the viability and apoptosis of HSC-3 cells.
Shen X., Zhang B., Hu X., Li J., Wu M., Yan C., Yang Y, & Li Y. (2022). Neisseria sicca and Corynebacterium matruchotii inhibited oral squamous cell carcinomas by regulating genome stability. Bioengineered, 13(6), 14094-14106.
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8

Adherent and Suspension CHO Cell Culture Protocols

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The adherent CHO-K1 (Invitrogen, Carlsbad, CA, USA) cells were maintained in high glucose DMEM supplemented with 10% FBS and incubated at 37 °C with 5% CO 2 in a humidified cell incubator (Thermo Fisher Scientific, Waltham, MA, USA) (Sun et al. 2015) (link). The suspension CHO-S (Invitrogen, Carlsbad, CA, USA) cells were cultured with CD CHO medium supplemented with 8 mM L-GlutaMax in 125-mL shake flasks (Corning, Corning, NY, USA) at 37 °C with 125 rpm and 8% CO 2 (Zong et al. 2017) (link). All media and supplements were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Cell growth and viability were monitored with a cell counter (Countstar, Shanghai, China).
Zhao M., Wang J., Luo M., Luo H., Zhao M., Han L., Zhang M., Yang H., Xie Y., Jiang H., Feng L., Lu H., & Zhu J. (2018). Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35. Applied Microbiology and Biotechnology, 102(14), 6105-6117.
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9

CRISPR/Cas9 Editing in HEK293T Cells

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Wild type HEK293 cells (HEK293T) were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and detected to be negative for mycoplasma contamination using the Myco-Blue mycoplasma detector (Vazyme; Nanjing, Jiangsu, China). Cells were cultured in high glucose DMEM supplemented with 10% FBS, incubated at 37ºC with 5% CO 2 in a humidified cell incubator (Thermo Fisher Scientific; OH, USA). The plasmid pX330 carrying CRISPR/Cas9 system was kindly provided by Dr. Feng Zhang (MIT) (L. Cong et al., 2013) .
Competent cells of the E. coli strains DH5α were purchased from Microgene (Shanghai, China). All media and supplements were purchased from Gibco (Thermo Fisher Scientific; Waltham, MA, USA). Cell growth and viability were monitored with a cell counter (Countstar; Shanghai, China).
Zhang M., Wang J., Tian Q., Feng L., Yang H., Zhu N., Pan X., Zhu J., Chen P., & Lu H. (2020). Analysis of genomic DNA methylation and gene transcription modulation induced by DNMT3A deficiency in HEK293 cells.
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